LOCUS       Exported                7074 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plant Gene Expression Vector.
ACCESSION   .
VERSION     .
KEYWORDS    pSIM24 KanR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    66..90
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      911..1552
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(2632..2811)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    3046..3070
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3185..3773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3265..3284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3944..4804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFCVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4567..4586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4805..4909)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5037..5568
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gcgcgcggta cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
       61 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
      121 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccactagac cagtaccttg
      181 gaggcctgtg cgcatcccag ggtgggtccc gtataccccg gggaattcgt caacttcgtc
      241 cacagacatc aacatcttat cgtcctttga agataagata ataatgttga agataagagt
      301 gggagccccc actaaaacat tgctttgtca aaagctaaaa aagatgatgc ccgacagcca
      361 cttgtgtgaa gcatgagaag ccggtccctc cactaagaaa attagtgaag catcttccag
      421 tggtccctcc actcacagct caatcagtga gcaacaggac gaaggaaatg acgtaagcca
      481 tgacgtctaa tcccaacttc gtccacagac atcaacatct tatcgtcctt tgaagataag
      541 ataataatgt tgaagataag agtgggagcc accactaaaa cattgctttg tcaaaagcta
      601 aaaaagatga tgcccgacag ccacttgtgt gaagcatgag aagccggtcc ctccactaag
      661 aaaattagtg aagcatcttc cagtggtccc tccactcaca gctcaatcag tgagcaacag
      721 gacgaaggaa atgacgtaag ccatgacgtc taatcccaca agaatttcct tatataagga
      781 acacaaatca gaaggaagag atcaatcgaa atcaaaatcg gaatcgaaat caaaatcgga
      841 atcgaaatct ctcatctaag cttggatccc tcgaggttaa cacgcgtgtc gacgagctcc
      901 tgcagtctag agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
      961 tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg cattgggaaa
     1021 actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc
     1081 gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt
     1141 gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa
     1201 attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta
     1261 atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact
     1321 aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt
     1381 cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca
     1441 gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa
     1501 atatttttta atgcatttta tgacttgcca attgattgac aacatgcatc aatcgatact
     1561 agtctagtaa catagatgac accgcgagcg ataatttatc ctagtttgcg agctatattt
     1621 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     1681 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     1741 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     1801 tgtttgaacg attcagaaga actcatcgag gagcctgtag aaagcaatcc tttgggaatc
     1861 tggagcagca attccgtaaa gcacaaggaa tctatcagcc cactctccac caagttcttc
     1921 agcaatatcc ctagtagcga gagcaatatc ctgataccta tcagcaactc caagccttcc
     1981 gcaatcaatg aatccagaaa accttccgtt ctccaccata atgtttggaa ggcaagcatc
     2041 accatgagta acaacaagat cctcaccatc tggcattcta gccttgagcc tagcaaaaag
     2101 ttcagctgga gccaatcctt gatgttcctc atcaagatca tcctgatcca caagtccagc
     2161 ttccatccta gttctagccc tttcaattct gtgcttagct tggtgatcaa atgggcaagt
     2221 agcaggatca agagtgtgaa gccttctcat agcatcagcc ataatggaaa ccttctcagc
     2281 tggagcaagg tgtgaagaaa gcaaatcttg tcctggaacc tctcccaaaa gaagccaatc
     2341 tcttccagcc tcagtaacaa catcaagaac agcagcgcat ggaacaccag tagtagcaag
     2401 ccaagaaagt ctagcagcct catcttgaag ctcattaaga gcaccagaaa gatcagtctt
     2461 aacgaaaaga actggccttc cctgagcaga aagcctaaaa acagcagcat cagagcatcc
     2521 aatagtctgt tgagcccaat cgtatccaaa aagcctctca acccaagcag caggacttcc
     2581 agcatgaagt ccatcttgct caattcccat ttgagagtga atatgagact ctaattggat
     2641 accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg
     2701 atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct
     2761 cattaaactc cagaaacccg cggctcagtg gctccttcaa cgttgcggtt ctgtcagttc
     2821 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
     2881 ctccgctcat gatcagatct ggtgacccct atgtaagggg ccggccattt aaattcgcga
     2941 ttaattaacc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
     3001 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
     3061 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatatcgcgc
     3121 gcggtacctg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4081 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacagaaa tgttgaatac
     4801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4921 gaaaagtgcc acgggccccg ctcaccgggc tggttgccct cgccgctggg ctggcggccg
     4981 tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc
     5041 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
     5101 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
     5161 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
     5221 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
     5281 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
     5341 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
     5401 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
     5461 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
     5521 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctgt cgactaccta
     5581 gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg
     5641 ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc tggtattcgt
     5701 gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga
     5761 cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga
     5821 ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg
     5881 caccttcgat cgcaaggcgt atcgccagga actgatcgac gccggcttct cggccgaaga
     5941 tgccgaaacg atcgcctcgc gcaccgtcat gcgtgccccg cgcgaaacct tccagtcggt
     6001 cggctcgatg gtccagcagg ccaccgccaa gatcgaacgc gattcggtcc agctggcccc
     6061 gccggccctt ccggccccgt cggccgccgt cgaacgctcg cgccggctgg aacaggaagc
     6121 cgccggcctg gccaagtcga tgaccatcga cacccgcggc accatgacca ccaagaagcg
     6181 caagaccgcc ggcgaggatc tggccaagca ggtcagcgaa gccaagcagg ccgccctgct
     6241 gaagcatacc aagcagcaga tcaaggaaat gcagctgtcg ctgttcgaca tcgccccgtg
     6301 gccggatacc atgcgggcca tgccgaatga taccgcccgc tcggccctgt tcaccacccg
     6361 caataagaag atcccgcgcg aagccctcca gaataaggtc atcttccacg tcaacaagga
     6421 tgtcaagatc acctataccg gcgtcgaact gcgggccgat gatgatgaac tggtctggca
     6481 gcaggtcctg gaatatgcca agcgcacccc gatcggcgaa ccgatcacct tcaccttcta
     6541 tgaactgtgc caggatctgg gctggtcgat caatggccgc tattatacca aggccgaaga
     6601 atgcctgtcg cgcctccagg cgaccgcgat gggcttcacc tcggatcgcg tcggccatct
     6661 ggaatcggtc agcctgctgc accgcttccg cgtcctggat cgcggcaaga agacctcgcg
     6721 ctgccaggtc ctgatcgacg aagaaatcgt cgtcctgttc gccggcgatc attataccaa
     6781 gttcatctgg gagaagtatc gcaagctgtc gccgaccgcc cgccgcatgt tcgattattt
     6841 ctcgtcgcac cgcgaaccgt atccgctgaa gctggaaacc ttccgcctga tgtgcggctc
     6901 ggattcgacc cgcgtcaaga agtggcgcga acaggtcggc gaagcctgcg aagaactgcg
     6961 cggctcgggc ctggtcgaac atgcctgggt caatgatgat ctggtccatt gcaagcgcta
     7021 gggccttgtg gggtcagttc cggctggggg ttcagccagc gctttacgat atcg
//
