LOCUS Exported 11560 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of TRIM33__RET fusion. ACCESSION . VERSION . KEYWORDS pHAGE-TRIM33__RET SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11560) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11560) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11560 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(5261..5272) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind complement(5506..5530) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6620..6640) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6694..6735 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6756..7323 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6773..7325 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6917..6934) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7144..7163 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7337..7936 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7337..7356) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7833..7853 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 7950..8538 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8003..8023) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8421..8432) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8613..8846 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8965..8983) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9051..9155 /gene="bla" /label=AmpR promoter CDS 9156..10016 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9374..9393) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10187..10775 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10676..10695 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10929..10946 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11038..11057 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11186..11207 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11222..11252 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11260..11276 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11265..11287 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11284..11300 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11284..11300 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11350..11369 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatggcg gaaaacaaag gcggcggcga ggctgagagc 3481 ggcggcgggg gcagcggcag cgcgccggta actgccgggg ccgccgggcc cgccgcgcag 3541 gaggcggagc cgcctctcac cgcggtgctg gtggaggagg aggaggagga aggcggcagg 3601 gccggcgctg agggcggcgc ggccgggccc gacgacgggg gggtggccgc ggcctcctcg 3661 ggctcggccc aggctgcttc atctcctgcg gcctcagtgg gcactggagt tgccgggggc 3721 gcagtatcga cgccggctcc agctccagcc tcggctcccg ctccgggtcc ctcggcaggg 3781 ccgcctcctg gaccgccagc ctcgctcctg gacacctgcg ccgtgtgtca gcagagcttg 3841 cagagccggc gtgaggcgga gcccaagctg ctgccctgtc ttcactcctt ctgcctgcgc 3901 tgcctgcccg agccggagcg ccagctcagc gtgcccatcc cggggggcag caacggcgac 3961 atccagcaag ttggtgtaat acggtgccca gtatgccgcc aagaatgcag acagatagac 4021 cttgtggata attattttgt gaaagacaca tctgaagctc ctagcagttc tgatgaaaaa 4081 tcagaacagg tatgtactag ttgtgaagac aatgcaagtg cagttggctt ttgtgtagaa 4141 tgtggagagt ggctatgtaa gacatgtatc gaagcacatc aaagagtaaa atttactaaa 4201 gatcacttga tcaggaagaa agaagatgtc tcagagtctg ttggagcatc tggtcaacgc 4261 cctgttttct gccctgtaca caaacaagaa cagttgaaac ttttctgtga aacatgtgat 4321 agattgacat gtagagactg tcagctattg gaacacaaag aacataggta tcagtttttg 4381 gaagaagctt ttcaaaatca gaagggtgca attgagaatc tactggcgaa acttcttgag 4441 aagaagaatt atgttcattt tgcagctact caggtgcaga ataggataaa agaagtaaat 4501 gagactaaca aacgagtaga acaggaaatt aaagtggcca ttttcaccct tatcaatgaa 4561 attaataaga aaggaaaatc tctcttacaa cagctagaga atgttacaaa ggaaagacag 4621 atgaagttac tacagcagca gaatgacatc acaggccttt cccggcaggt gaagcatgtt 4681 atgaacttca caaattgggc aattgcaagt ggcagcagca cagcactact atacagcaag 4741 cgactgatta ctttccagtt gcgtcatatt ttgaaagcac ggtgtgatcc tgtccctgct 4801 gctaatggag caatacgttt ccattgtgat cccaccttct gggcaaagaa tgtagtcaat 4861 ttaggtaatc tagtaataga gagtaaacca gctcctggtt atactcctaa tgttgtagtt 4921 gggcaagttc ctccagggac aaaccacatt agtaaaaccc ctggacagat taacttagca 4981 cagcttcgac tccagcacat gcaacaacaa gtatatgcac agaaacatca gcagttgcaa 5041 cagatgagga tgcagcaacc accagcacct gtaccaacta caacaacaac aacacaacag 5101 catcctagac aagcagcccc tcagatgtta caacaacagc ctcctcgatt gatcagtgtg 5161 caaacaatgc aaagaggcaa catgaactgt ggagcttttc aagcccatca gatgagactg 5221 gctcagaatg ctgccagaat accagggata cccaggcaca gcggccctca atattccatg 5281 atgcagccac acctccaaag acaacactca aacccagggc atgctggacc ctttcccgta 5341 gtatcggtac acaacaccac aatcaaccca acgagcccta ctacagcaac tatggcaaat 5401 gcaaaccgag gtcccaccag cccatctgtt acagcaatag agctaatccc ctcagttacc 5461 aatccagaaa accttccatc gctgccagat attccaccca tacagtccca gctttcttgt 5521 acaaagtggt tcgaggatcc aaagtgggaa ttccctcgga agaacttggt tcttggaaaa 5581 actctaggag aaggcgaatt tggaaaagtg gtcaaggcaa cggccttcca tctgaaaggc 5641 agagcagggt acaccacggt ggccgtgaag atgctgaaag agaacgcctc cccgagtgag 5701 cttcgagacc tgctgtcaga gttcaacgtc ctgaagcagg tcaaccaccc acatgtcatc 5761 aaattgtatg gggcctgcag ccaggatggc ccgctcctcc tcatcgtgga gtacgccaaa 5821 tacggctccc tgcggggctt cctccgcgag agccgcaaag tggggcctgg ctacctgggc 5881 agtggaggca gccgcaactc cagctccctg gaccacccgg atgagcgggc cctcaccatg 5941 ggcgacctca tctcatttgc ctggcagatc tcacagggga tgcagtatct ggccgagatg 6001 aagctcgttc atcgggactt ggcagccaga aacatcctgg tagctgaggg gcggaagatg 6061 aagatttcgg atttcggctt gtcccgagat gtttatgaag aggattcgta cgtgaagagg 6121 agccagggtc ggattccagt taaatggatg gcaattgaat ccctttttga tcatatctac 6181 accacgcaaa gtgatgtatg gtcttttggt gtcctgctgt gggagatcgt gaccctaggg 6241 ggaaacccct atcctgggat tcctcctgag cggctcttca accttctgaa gaccggccac 6301 cggatggaga ggccagacaa ctgcagcgag gagatgtact gcctgatgct gcaatgctgg 6361 aagcaggagc cggacaaaag gccggtgttt gcggacatca gcaaagacct ggagaagatg 6421 atggttaaga ggagagacta cttggacctt gcggcgtcca ctccatctga ctccctgatt 6481 tatgacgacg gcctctcaga ggaggagaca ccgctggtgg actgtaataa tgcccccctc 6541 cctcgagccc tcccttccac atggattgaa aacaaactct atggtagaat ttcccatgca 6601 tttactagat tctagcaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc 6661 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc 6721 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact 6781 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 6841 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 6901 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 6961 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 7021 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 7081 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 7141 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 7201 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 7261 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 7321 taatatggcc acacatatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 7381 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 7441 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 7501 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 7561 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 7621 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 7681 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 7741 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 7801 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 7861 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 7921 caagcccggt gcctaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa 7981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 8041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 8101 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 8161 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 8221 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 8281 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 8341 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 8401 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 8461 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 8521 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga 8581 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 8641 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 8701 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 8761 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 8821 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 8881 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc 8941 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 9001 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 9061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 9121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 9181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 9241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 9301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 9361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 9421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 9481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 9541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 9601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 9661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 9721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 9781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 9841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 9901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 9961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 10021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 10081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 10141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 10201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 10261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 10321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 10381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 10441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 10501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 10561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 10621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 10681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 10741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 10801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 10861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 10921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 10981 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat 11041 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 11101 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga 11161 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 11221 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 11281 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc 11341 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 11401 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 11461 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 11521 gaaattgtat ttgttaaata tgtactacaa acttagtagt //