LOCUS       Exported               10259 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of PPP2R1A.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-PPP2R1A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3446..3455
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(5219..5243)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5251..5269
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5294..5313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5295..5313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5319..5339)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5393..5434
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5455..6022
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5472..6024
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5616..5633)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5843..5862
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6036..6635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6036..6055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6532..6552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6649..7237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6702..6722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7120..7131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7312..7545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7664..7682)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7750..7854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7855..8715
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8073..8092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8886..9474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9375..9394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9628..9645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9737..9756
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    9885..9906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9921..9951
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9959..9975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9964..9986
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9983..9999
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9983..9999
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10049..10068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcggcggccg acggcgacga ctcgctgtac
     3481 cccatcgcgg tgctcataga cgaactccgc aatgaggacg ttcagcttcg cctcaacagc
     3541 atcaagaagc tgtccaccat cgccttggcc cttggggttg aaaggacccg aagtgagctt
     3601 ctgcctttcc ttacagatac catctatgat gaagatgagg tcctcctggc cctggcagaa
     3661 cagctgggaa ccttcactac cctggtggga ggcccagagt acgtgcactg cctgctgcca
     3721 ccgctggagt cgctggccac agtggaggag acagtggtgc gggacaaggc agtggagtcc
     3781 ttacgggcca tctcacacga gcactcgccc tctgacctgg aggcgcactt tgtgccgcta
     3841 gtgaagcggc tggcgggcgg cgactggttc acctcccgca cctcggcctg cggcctcttc
     3901 tccgtctgct acccccgagt gtccagtgct gtgaaggcgg aacttcgaca gtacttccgg
     3961 aacctgtgct cagatgacac ccccatggtg cggcgggccg cagcctccaa gctgggggag
     4021 tttgccaagg tgctggagct ggacaacgtc aagagtgaga tcatccccat gttctccaac
     4081 ctggcctctg acgagcagga ctcggtgcgg ctgctggcgg tggaggcgtg cgtgaacatc
     4141 gcccagcttc tgccccagga ggatctggag gccctggtga tgcccactct gcgccaggcc
     4201 gctgaagaca agtcctggcg cgtccgctac atggtggctg acaagttcac agagctccag
     4261 aaagcagtgg ggcctgagat caccaagaca gacctggtcc ctgccttcca gaacctgatg
     4321 aaagactgtg aggccgaggt gagggccgca gcctcccaca aggtcaaaga gttctgtgaa
     4381 aacctctcag ctgactgtcg ggagaatgtg atcatgtccc agatcttgcc ctgcatcaag
     4441 gagctggtgt ccgatgccaa ccaacatgtc aagtctgccc tggcctcagt catcatgggt
     4501 ctctctccca tcttgggcaa agacaacacc atcgagcacc tcttgcccct cttcctggct
     4561 cagctgaagg atgagtgccc tgaggtacgg ctgaacatca tctctaacct ggactgtgtg
     4621 aacgaggtga ttggcatccg gcagctgtcc cagtccctgc tccctgccat tgtggagctg
     4681 gctgaggacg ccaagtggcg ggtgcggctg gccatcattg agtacatgcc cctcctggct
     4741 ggacagctgg gagtggagtt ctttgatgag aaacttaact ccttgtgcat ggcctggctt
     4801 gtggatcatg tatatgccat ccgcgaggca gccaccagca acctgaagaa gctagtggaa
     4861 aagtttggga aggagtgggc ccatgccaca atcatcccca aggtcttggc catgtccgga
     4921 gaccccaact acctgcaccg catgactacg ctcttctgca tcaatgtgct gtctgaggtc
     4981 tgtgggcagg acatcaccac caagcacatg ctacccacgg ttctgcgcat ggctggggac
     5041 ccggttgcca atgtccgctt caatgtggcc aagtctctgc agaagatagg gcccatcctg
     5101 gacaacagca ccttgcagag tgaagtcaag cccatcctag agaagctgac ccaggaccag
     5161 gatgtggacg tcaaatactt tgcccaggag gctctgactg ttctgtctct cgcctagaac
     5221 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaagga tagatttaag
     5281 tgaagagagt tatccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt
     5341 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     5401 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     5461 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     5521 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     5581 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     5641 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     5701 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5761 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5821 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5881 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5941 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6001 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc
     6061 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6121 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6181 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6241 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6301 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6361 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6421 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6481 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6601 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg
     6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7201 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     7261 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7321 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7561 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     7621 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     7681 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     8101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
     9721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9781 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
     9841 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9901 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9961 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    10021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10081 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    10141 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    10201 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
