LOCUS Exported 10546 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of GSK3A__ZNF564 fusion. ACCESSION . VERSION . KEYWORDS pHAGE-GSK3A__ZNF564 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10546) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10546) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10546 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3916..3940) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5606..5626) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5680..5721 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5742..6309 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5759..6311 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5903..5920) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6130..6149 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6323..6922 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6323..6342) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6819..6839 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6936..7524 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6989..7009) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7407..7418) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7599..7832 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7951..7969) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8037..8141 /gene="bla" /label=AmpR promoter CDS 8142..9002 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8360..8379) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9173..9761 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9662..9681 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9915..9932 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10024..10043 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10172..10193 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10208..10238 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10246..10262 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10251..10273 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10270..10286 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10270..10286 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10336..10355 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatgagc ggcggcgggc cttcgggagg cggccctggg 3481 ggctcgggca gggcgcggac tagctcgttc gcggagcccg gcggcggagg cggaggaggc 3541 ggcggcggcc ccggaggctc ggcctccggc ccaggcggca ccggcggcgg aaaggcatct 3601 gtcggggcca tgggtggggg cgtcggggcc tcgagctccg ggggtggacc cggcggcagc 3661 ggcggaggag gcagcggagg ccccggcgca ggcactagct tcccgccgcc cggggtgaag 3721 ctgggccgtg acagcgggaa ggtgaccaca gtcgtagcca ctctaggcca aggcccagag 3781 cgctcccaag aagtggctta cacggacatc aaagtgattg gcaatggctc atttggggtc 3841 gtgtaccagg cacggctggc agagaccagg gaactagtcg ccatcaagaa ggttctccag 3901 gacaagaggt tcaagtccca gctttcttgt acaaagtggt tcgactcagt ggcctctgag 3961 gatgtggctg tgaacttcac acttgaggag tgggctttgc tggatccttc ccagaagaaa 4021 ctctacagag atgtgatgcg ggaaaccttt agaaacctgg cctgtgtagg aaaaaaatgg 4081 gaagaccaga gcattgaaga ttggtacaaa aatcagggga gaattttaag aaatcatatg 4141 gaagagggac tcagtgaaag taaagaatat gatcaatgtg gagaagcctt cagtcagatt 4201 ctcaatctta atctgaacaa gaaaattcct actatagtaa gaccatgtga atgtagtttg 4261 tgtgggaaag tcttcatgca tcattcatcc cttagtaggc acatcagatc tcaccttgga 4321 cacaaaccat atgactatca ggaatatgga gagaaaccat ataaatgtaa gcagtgtggg 4381 aaagccttca gttcttgtca atcctttcga agacatgaaa gaactcacac tggtgagaaa 4441 ccctatgcat gtccggaatg tgggaaagcc ttcatttctc tcccaagtgt tcgaagacac 4501 atgattaagc acactggaga tggaccatat aaatgtcagg aatgtgggaa agcctttgat 4561 cgcccaagtt tatttcagat acatgaaaga actcacactg gagagaaacc ctatgaatgt 4621 caggaatgtg caaaagcttt catttctctt ccaagttttc aaagacacat gattaggcac 4681 actggagatg gaccttataa atgtcaggaa tgtggaaaag cctttgaccg ccccagttta 4741 tttcgaatac atgaaagaac tcatactgga gagaaacccc atgaatgtaa acagtgtggg 4801 aaggccttca tttctttcac aaattttcaa agtcatatga ttaggcacac tggggatgga 4861 ccttataaat gtaaagtatg tgggagagcc tttatttttc ccagttatgt tcgaaagcat 4921 gaaagaactc atactgggga gaaaccctat gaatgtaata aatgtggtaa aaccttcagt 4981 tcttccagta atgttcgaac acatgaaagg actcacactg gagagaagcc ctatgaatgt 5041 aaggaatgcg ggaaagcctt catttctctc ccaagtgtcc gaagacacat gataaagcac 5101 actggagatg gaccttataa atgtcaggta tgtggtagag cctttgactg tcccagttca 5161 tttcaaatac acgaaagaac tcacactgga gagaaaccct atgaatgtca ggtatgtggg 5221 aaagccttca tttctcttaa aaggattaga aaacatatga tactgcacac tggagatgga 5281 ccttataaat gtcaggtatg tggtaaagcc tttgactgtc ctagttctgt ccgaacacat 5341 gaaagaactc atactggaga aaaaccctat gaatgtaaag aatgtgggaa agcattcaat 5401 tatgccagtt ccattagaat acatgaaaga actcataccg gagaaaaacc ctatgaatgt 5461 aagcaatgtg gaaaaacgtt cagttattcc agttcctttc aaagacatga aagagctcat 5521 aatggagata aaccttacgt aaagaatgtg ggaaagcttt catttatcac acaaccttcg 5581 aatacctgtg aaaatgaata gcacccaact tttctataca aagtggttga tatccagcac 5641 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct 5701 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc cccccctaac 5761 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc 5821 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg 5881 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg 5941 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc 6001 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa 6061 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa 6121 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta 6181 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg 6241 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 6301 cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc tcgccacccg 6361 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac 6421 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt 6481 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt 6541 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc 6601 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct 6661 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga 6721 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg 6781 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg 6841 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat 6901 gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat tacaaaattt 6961 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 7021 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 7081 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 7141 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 7201 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 7261 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 7321 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 7381 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 7441 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 7501 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc 7561 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 7621 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 7681 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 7741 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 7801 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 7861 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga 7921 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta 7981 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 8041 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 8101 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 8161 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 8221 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 8281 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 8341 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag 8401 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 8461 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 8521 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 8581 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 8641 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 8701 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 8761 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 8821 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 8881 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 8941 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 9001 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 9061 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 9121 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 9181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 9241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 9301 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 9361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 9421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 9481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 9541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 9601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 9661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 9721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 9781 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 9841 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 9901 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 9961 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt 10021 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 10081 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg 10141 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 10201 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 10261 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt 10321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca 10381 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca 10441 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat 10501 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //