LOCUS Exported 10057 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of STK33. ACCESSION . VERSION . KEYWORDS pHAGE-STK33 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10057) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10057) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10057 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4994..5018) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5071..5112 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5133..5700 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5150..5702 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5294..5311) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5521..5540 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5714..6433 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5714..6430 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5759..5780) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6020..6039) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6367..6388 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6447..7035 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6500..6520) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6918..6929) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7110..7343 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7462..7480) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7548..7652 /gene="bla" /label=AmpR promoter CDS 7653..8513 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7871..7890) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8684..9272 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9173..9192 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9426..9443 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9535..9554 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9683..9704 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9719..9749 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9757..9773 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9762..9784 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9781..9797 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9781..9797 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9847..9866 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gctgatagtg gcttagataa aaaatccaca 3481 aaatgccccg actgttcatc tgcttctcag aaagatgtac tttgtgtatg ttccagcaaa 3541 acaagggttc ctccagtttt ggtggtggaa atgtcacaga catcaagcat tggtagtgca 3601 gaatctttaa tttcactgga gagaaaaaaa gaaaaaaata tcaacagaga tataacctcc 3661 aggaaagatt tgccctcaag aacctcaaat gtagagagaa aagcatctca gcaacaatgg 3721 ggtcggggca actttacaga aggaaaagtt cctcacataa ggattgagaa tggagctgct 3781 attgaggaaa tctatacctt tggaagaata ttgggaaaag ggagctttgg aatagtcatt 3841 gaagctacag acaaggaaac agaaacgaag tgggcaatta aaaaagtgaa caaagaaaag 3901 gctggaagct ccgctgtgaa gttacttgaa cgagaggtga acattctgaa aagtgtaaaa 3961 catgaacaca tcatacatct ggaacaagta tttgaaacgc caaagaaaat gtaccttgtg 4021 atggagcttt gtgaggatgg agaactcaaa gaaattctgg ataggaaagg gcatttctca 4081 gagaatgaga caaggtggat cattcaaagt ctcgcatcag ctatagcata tcttcacaat 4141 aatgatattg tacataggga tctgaaactg gaaaatataa tggttaaaag cagtcttatt 4201 gatgataaca atgaaataaa cttaaacata aaggtgactg attttggctt agcggtgaag 4261 aagcaaagta ggagtgaagc catgctgcag gccacatgtg ggactcctat ctatatggcc 4321 cctgaagtta tcagtgccca cgactatagc cagcagtgtg acatttggag cataggcgtc 4381 gtaatgtaca tgttattacg tggagaacca ccctttttgg caagctcaga agagaagctt 4441 tttgagttaa taagaaaagg agaactacat tttgaaaatg cagtctggaa ttccataagt 4501 gactgtgcta aaagtgtttt gaaacaactt atgaaagtag atcctgctca cagaatcaca 4561 gctaaggaac tactagataa ccagtggtta acaggcaata aactttcttc ggtgagacca 4621 accaatgtat tagagatgat gaaggaatgg aaaaataacc cagaaagtgt tgaggaaaac 4681 acaacagaag agaagaataa gccgtccact gaagaaaagt tgaaaagtta ccaaccctgg 4741 ggaaatgtcc ctgagaccaa ttacacttca gatgaagagg aggaaaaaca gtctactact 4801 tatgaaaagc aatttcctgc aaccagtaag gacaactttg atatgtgcag ttcaagtttc 4861 acatctagca aactccttcc agctgaaatc aagggagaaa tggagaaaac ccctgtgact 4921 ccaagccaag gaacagcaac caagtaccct gctaaatccg gcgccctgtc cagaaccaaa 4981 aagaaacttt agaacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc 5041 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt 5101 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc 5161 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 5221 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 5281 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 5341 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 5401 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 5461 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 5521 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 5581 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 5641 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 5701 tatggccaca catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 5761 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 5821 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 5881 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 5941 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 6001 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 6061 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 6121 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 6181 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 6241 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 6301 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 6361 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 6421 gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat 6481 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 6541 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 6601 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 6661 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 6721 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 6781 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 6841 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 6901 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6961 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 7021 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct 7081 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 7141 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 7201 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7261 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7321 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7381 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag 7441 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 7501 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 7561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 7621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 7681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 7741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 7801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 7861 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg 7921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 7981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 8041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 8101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 8161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 8221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 8281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 8341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 8401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 8461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 8521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 8581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 8641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 8701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 8761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 8821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 8881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 8941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 9001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 9061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 9121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 9181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 9241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 9301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 9361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 9421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 9481 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta 9541 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 9601 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag 9661 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 9721 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 9781 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt 9841 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa 9901 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt 9961 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa 10021 attgtatttg ttaaatatgt actacaaact tagtagt //