LOCUS       Exported               11381 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of FGFR3__TACC3 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-FGFR3__TACC3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6292..6315
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(6318..6342)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6395..6436
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6457..7024
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6474..7026
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6618..6635)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6845..6864
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7038..7757
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7038..7754
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7083..7104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7344..7363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7691..7712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7771..8359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7824..7844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8242..8253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8434..8667
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8786..8804)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8872..8976
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8977..9837
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9195..9214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10008..10596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10497..10516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10750..10767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10859..10878
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    11007..11028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11043..11073
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11081..11097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11086..11108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11105..11121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11105..11121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11171..11190
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggga gcaccagctt gtgccctggc tctgtgtgtg
     3481 gccgtggcta ttgtggctgg cgcctctagc gagtctctgg gcacagagca gagagtcgtg
     3541 ggcagagccg ctgaagtgcc tggacctgag cctggccagc aagagcagct ggtgtttggc
     3601 tctggcgacg ccgtggaact gagctgtcct cctcctggtg gcggccctat gggccctaca
     3661 gtgtgggtta aggacggcac cggactggtg cctagcgaga gagtgctcgt tggccctcag
     3721 agactgcagg tcctgaacgc ctctcacgag gactccggcg cctacagctg cagacagagg
     3781 ctgacccagc gggtgctgtg ccacttctcc gtcagagtga ccgatgcccc tagcagcggc
     3841 gacgacgagg atggcgagga tgaggccgaa gataccggcg tggacacagg cgccccttac
     3901 tggaccagac ccgagcggat ggacaagaaa ctgctggccg tgcctgccgc caacaccgtg
     3961 cggtttagat gcccagccgc cggaaacccc accccttcca tcagctggct gaagaacgga
     4021 agagagttcc ggggcgagca ccggatcggc ggcattaagc tgagacacca gcagtggtcc
     4081 ctggtcatgg aaagcgtggt gccctccgac cggggcaact acacctgtgt ggtggaaaac
     4141 aagttcggca gcatccggca gacctacacc ctggacgtgc tggaaagaag ccctcacaga
     4201 cccatcctgc aggccggact gcctgccaat cagacagccg tgctgggcag cgacgtggaa
     4261 tttcactgca aggtgtacag cgacgcccag ccccacatcc agtggctgaa acacgtggaa
     4321 gtgaacggca gcaaagtggg ccccgacggc acaccttacg tgaccgtgct gaaaacagcc
     4381 ggcgctaaca ccaccgacaa agaactggaa gtgctgtccc tgcacaacgt gaccttcgag
     4441 gatgccggcg agtacacatg tctggccggc aacagcatcg gcttctccca ccattctgcc
     4501 tggctggtgg tgctgcccgc cgaagaggaa ctggtggaag ccgacgaggc cggaagcgtg
     4561 tacgccggaa tcctgagcta cggcgtgggc tttttcctgt tcatcctggt tgtggccgcc
     4621 gtgaccctgt gcagactgag aagcccacct aagaagggcc tgggctcccc taccgtgcac
     4681 aagatcagcc ggttccctct gaagagacag gtgtccctgg aaagcaacgc ctccatgagc
     4741 agcaacaccc ctctcgtgcg gatcgccaga ctgtctagcg gcgaaggccc aaccctggcc
     4801 aacgtgtcag agctggaact gccagccgac cccaagtggg agctgagcag agctagactg
     4861 accctgggca agcctctggg cgagggctgc tttggacagg ttgtgatggc cgaggccatc
     4921 ggcatcgata aggatagagc cgccaagccc gtgacagtgg cagtgaagat gctgaaggac
     4981 gacgccacag acaaggacct gtccgacctg gtgtccgaga tggaaatgat gaagatgatc
     5041 ggcaagcaca agaacatcat caatctgctg ggagcctgca cccagggcgg acccctgtat
     5101 gtgctggtcg aatacgccgc caaaggcaac ctgcgcgagt tcctgagagc cagaaggcct
     5161 cccggcctgg actacagctt cgatacctgc aagccacccg aggaacagct gacctttaag
     5221 gacctcgtgt cctgcgccta tcaggtggcc agaggcatgg aatacctggc ctcccagaag
     5281 tgcatccacc gggacctggc cgccagaaac gtgctcgtga ccgaggacaa cgtcatgaag
     5341 atcgccgatt tcggcctggc ccgggatgtg cacaatctgg attactacaa gaaaaccacc
     5401 aacggccggc tgccagtgaa gtggatggcc cctgaggccc tgttcgacag agtgtacacc
     5461 caccagtctg acgtgtggtc cttcggcgtg ctgctgtggg agatcttcac actgggcggc
     5521 agcccctacc ctggcatccc cgtggaagaa ctgttcaagc tgctgaaaga gggccaccgg
     5581 atggataagc ccgccaattg cacccacgac ctgtatatga tcatgcggga atgctggcac
     5641 gccgctccaa gccagcggcc taccttcaaa cagctggttg aggacctgga cagggtgctg
     5701 accgtgacct ccaccgacgt gaaggccacc caagaggaaa accgcgagct gcggagcaga
     5761 tgcgaggaac tccacggcaa gaacctggaa ctcggcaaga tcatggacag attcgaagag
     5821 gtggtgtacc aggccatgga agaggtccag aagcagaaag agctgtctaa ggccgagatc
     5881 cagaaagtcc tgaaagaaaa ggaccagctc accacggacc tgaacagcat ggaaaagagc
     5941 ttcagcgacc tgtttaagcg cttcgaaaaa cagaaagaag tgatcgaggg ctatcggaag
     6001 aacgaggaat ccctgaagaa gtgcgtcgag gactacctgg ctcggatcac acaagagggg
     6061 cagcggtatc aggccctgaa ggctcacgcc gaggaaaaac tccagctggc caatgaagag
     6121 attgcccaag tgcggtccaa ggcccaggct gaagcactgg cactgcaggc ttccctgcgg
     6181 aaagaacaga tgcggatcca gagcctggaa aaaacagtcg agcaaaagac caaagagaac
     6241 gaagaactca cccggatctg cgacgacctg atcagcaaga tggaaaaaat cgactacaag
     6301 gacgatgacg acaagtgacc caactttctt gtacaaagtg gttgatatcc agcacagtgg
     6361 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     6421 cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac
     6481 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6541 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6601 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6661 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6721 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6781 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6841 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6901 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6961 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     7021 ataatatggc cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     7081 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     7141 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     7201 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     7261 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     7321 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     7381 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     7441 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     7501 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7561 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7621 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7681 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7741 acgagctgta caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa
     7801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8161 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8281 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8341 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
     8401 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8461 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8521 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8581 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8641 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8701 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
     8761 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8821 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8881 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8941 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9001 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9061 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9121 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9181 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     9241 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9301 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9361 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9421 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9481 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9541 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9601 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9661 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9721 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9781 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9841 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9901 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10801 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    10861 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10921 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    10981 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    11041 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    11101 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    11161 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    11221 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11281 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    11341 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
