LOCUS Exported 9787 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of WDR12. ACCESSION . VERSION . KEYWORDS pHAGE-WDR12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9787) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9787) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9787 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4724..4748) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4801..4842 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4863..5430 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4880..5432 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5024..5041) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5251..5270 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5444..6163 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5444..6160 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5489..5510) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5750..5769) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6097..6118 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6177..6765 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6230..6250) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6648..6659) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6840..7073 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7192..7210) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7278..7382 /gene="bla" /label=AmpR promoter CDS 7383..8243 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7601..7620) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8414..9002 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8903..8922 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9156..9173 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9265..9284 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9413..9434 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9449..9479 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9487..9503 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9492..9514 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9511..9527 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9511..9527 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9577..9596 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggctccacc atggctcagc tccaaacacg cttctacact 3481 gataacaaga aatatgccgt agatgatgtt cccttctcaa tccctgctgc ctctgaaatt 3541 gccgacctta gtaacatcat caataaacta ctaaaggaca aaaatgagtt ccacaaacat 3601 gtggagtttg atttccttat taagggccag tttctgcgaa tgcccttgga caaacacatg 3661 gaaatggaga acatctcatc agaagaagtt gtggaaatag aatacgtgga gaagtatact 3721 gcaccccagc cagagcaatg catgttccat gatgactgga tcagttcaat taaaggggca 3781 gaggaatgga tcttgactgg ttcttatgat aagacttctc ggatctggtc cttggaagga 3841 aagtcaataa tgacaattgt gggacatacg gatgttgtaa aagatgtggc ctgggtgaaa 3901 aaagatagtt tgtcctgctt attattgagt gcttctatgg atcagactat tctcttatgg 3961 gagtggaatg tagagagaaa caaagtgaaa gccctacact gctgtagagg tcatgctgga 4021 agtgtagatt ctatagctgt tgatggctca ggaactaaat tttgcagtgg ctcctgggat 4081 aagatgctaa agatctggtc tacagtccct acagatgaag aagatgaaat ggaggagtcc 4141 acaaatcgac caagaaagaa acagaagaca gaacagttgg gactaacaag gactcccata 4201 gtgaccctct ctggccacat ggaggcagtt tcctcagttc tgtggtcaga tgctgaagaa 4261 atctgcagtg catcttggga ccatacaatt agagtgtggg atgttgagtc tggcagtctt 4321 aagtcaactt tgacaggaaa taaagtgttt aattgtattt cctattctcc actttgtaaa 4381 cgtttagcat ctggaagcac agataggcat atcagactgt gggatccccg aactaaagat 4441 ggttctttgg tgtcgctgtc cctaacgtca catactggtt gggtgacatc agtaaaatgg 4501 tctcctaccc atgaacagca gctgatttca ggatctttag ataacattgt taagctgtgg 4561 gatacaagaa gttgtaaggc tcctctctat gatctggctg ctcatgaaga caaagttctg 4621 agtgtagact ggacagacac agggctactt ctgagtggag gagcagacaa taaattgtat 4681 tcctacagat attcacctac cacttcccat gttggggcat aggacccagc tttcttgtac 4741 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa 4801 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga 4861 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg 4921 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa 4981 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg 5041 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca 5101 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc 5161 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt 5221 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg 5281 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca 5341 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac 5401 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatggtga gcaagggcga 5461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 5521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 5581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 5641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 5701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 5761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 5821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 5881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 5941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 6001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 6061 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 6121 cgccgccggg atcactctcg gcatggacga gctgtacaag taaatcgata gatcctaatc 6181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 6241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 6301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 6361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 6421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 6481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 6541 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 6601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 6721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag 6781 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6841 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 6901 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6961 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7021 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7081 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7141 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga 7201 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 7261 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 7321 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 7381 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 7441 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 7501 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 7561 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 7621 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 7681 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 7741 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 7801 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 7861 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 7921 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 7981 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 8041 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 8101 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 8161 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 8221 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 8281 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 8341 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 8401 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 8461 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 8521 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 8581 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 8641 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 8701 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 8761 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 8821 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 8881 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 8941 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 9001 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 9061 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 9121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 9181 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca 9241 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 9301 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 9361 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 9421 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 9481 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt 9541 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 9601 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 9661 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 9721 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 9781 tagtagt //