LOCUS Exported 6363 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV-mediated expression of ChromeQ-GFP under the Syn promoter.. ACCESSION . VERSION . KEYWORDS pAAV-Syn-ChromeQ-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6363) AUTHORS Cho YK, Park D, Yang A, Chen F, Chuong AS, Klapoetke NC, Boyden ES TITLE Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents. JOURNAL J Biol Chem. 2019 Jan 4. pii: RA118.006996. doi: 10.1074/jbc.RA118.006996. PUBMED 30610117 REFERENCE 2 (bases 1 to 6363) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6363 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 341..788 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 801..810 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1755..2468 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(1797..1818) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2058..2077) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2405..2426 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2496..3084 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2549..2569) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2967..2978) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3104..3580 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3303..3322) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3626..3766 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3626..3755 /label=AAV2 ITR rep_origin 3841..4296 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3928..3947) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4138..4159 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4313..4332) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4432..4454 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4492..4510) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4578..4682 /gene="bla" /label=AmpR promoter CDS 4683..5543 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4901..4920) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5714..6302 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6203..6222 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc 181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc 241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca 301 aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga 361 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc 421 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga 481 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc 541 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc 601 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg 661 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg 721 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga 781 gagcgcagtc gagaggatcc gccaccatgg actatggcgg cgctttgtct gccgtcggac 841 gcgaactttt gttcgttact aatcctgtgg tggtgaacgg gtccgtcctg gtccctgagg 901 atcaatgtta ctgtgccgga tggattgaat ctcgcggcac gaacggcgct cagaccgcgt 961 caaatgtcct gcagtggctt gcagcaggat tcagcatttt gctgctgatg ttctattctt 1021 accaaacctg gaaatctaca tgcggctggg aggagatcta tgtgtgcgcc attgcaatgg 1081 ttaaggtgat tctcgagttc ttttttgagt ttaagaatcc ctctatgctc taccttgcca 1141 caggaggttc ggtgcagtgg ctgcgctatg cagagtggct gctcacttgt cctgtcatcc 1201 ttatccacct gagcaacctc accggcctga gcaacgacta cagcaggaga accatgggac 1261 tccttgtctc agacatcggg actatcgtgt ggggggctac cagcgccatg gcaaccggct 1321 atgttaaagt catcttcttt tgtcttggat tgtgctatgg cgcgaacaca ttttttcacg 1381 ccgccaaagc atatatcgag ggttatcata ctgtgccaaa gggtcggtgc cgccaggtcg 1441 tgaccggcat ggcatggctg tttttcgtga gctggggtat gttcccaatt ctcttcattt 1501 tggggcccga aggttttggc gtcctgagcg tctatggctc caccgtaggt cacacgatta 1561 ttgatctgat gagtaaaaat tgttgggggt tgttgggaca ctacctgcgc gtcctgatcc 1621 acgagcacat attgattcac ggagatatcc gcaaaaccac caaactgaac atcggcggaa 1681 cggagatcga ggtcgagact ctcgtcgaag acgaagccga ggccggagcc gtgccagcgg 1741 caccggtagt agcagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 1801 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 1861 atgccaccta cggcaagctg accctgaagt tcatttgcac caccggcaag ctgcccgtgc 1921 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 1981 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 2041 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 2101 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 2161 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 2221 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 2281 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 2341 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 2401 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 2461 tgtacaagta agaattccgt acaaagctta tcgataatca acctctggat tacaaaattt 2521 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 2581 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 2641 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 2701 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 2761 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 2821 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 2881 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc 2941 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 3001 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 3061 tctccctttg ggccgcctcc ccgcatcgat accggctcga gacgggtggc atccctgtga 3121 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt 3181 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg 3241 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg 3301 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc 3361 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg 3421 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc 3481 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct 3541 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctcgagctga ttttgtaggt 3601 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct 3661 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 3721 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg 3781 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt 3841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 3901 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 3961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4021 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc 4081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4141 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat 4201 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta 4261 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct 4321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 4381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 4441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 4501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 4561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 4621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 4681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 4921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 4981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 5041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 5101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 5161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 5221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 5281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 5341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 5401 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 5461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 5521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 5581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 5641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 5701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 5881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 5941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 6001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 6061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 6121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 6181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 6241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 6301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 6361 tgt //