LOCUS       Exported               13927 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with
            increased virulence in Agrobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pSB90 - pAGM4723_VirGN54D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13927)
  AUTHORS   Mortensen S., Bernal-Franco D., Cole LF., Sathitloetsakun S., Cram 
            EJ., Lee-Parsons CW.
  TITLE     EASI transformation: An efficient transient expression method for 
            analyzing gene function in Catharanthus roseus seedlings
  JOURNAL   frontiers in Plant Science, 2019
REFERENCE   2  (bases 1 to 13927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13927
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             102..1175
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1241..1435
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1497..1601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1602..2396
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3721..4309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4210..4229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4445..4469
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      10653..10662
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    12667..12691
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12971..13600
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
       61 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
      121 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
      181 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
      241 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgtcgtcga
      301 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
      361 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
      421 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
      481 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
      541 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
      601 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
      661 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
      721 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
      781 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
      841 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
      901 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
      961 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     1021 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     1081 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     1141 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     1201 ttgccctagc aggggaaaaa ggtcgaaaaa gcttctttcc tgtggatagc acgtacattg
     1261 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     1321 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     1381 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     1441 ctggccagcg cacagccgaa cagctgcaaa aagcgcccac atcaaggctc cgagtgcgcg
     1501 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1561 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatggctaaa atgagaatat
     1621 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
     1681 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
     1741 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
     1801 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
     1861 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
     1921 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
     1981 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
     2041 tgaataacga tctggccgat gtggattgcg aaaactggga agaggacact ccatttaaag
     2101 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
     2161 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
     2221 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
     2281 cgctcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
     2341 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagctgt
     2401 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2461 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2521 cgttctagct gtaacctcga agcgtttcac ttgtaacaac gattgagaat ttttgtcata
     2581 aaattgaaat acttggttcg catttttgtc atccgcggtc agccgcaatt ctgacgaact
     2641 gcccatttag ctggagatga ttgtacatcc ttcacgtgaa aatttctcaa gcgctgtgaa
     2701 caagggttca gattttagat tgaaaggtga gccgttgaaa cacgttcttc ttgtcgatga
     2761 cgacgtcgct atgcggcatc ttattattga ataccttacg atccacgcct tcaaagtgac
     2821 cgcggtagcc gacagcaccc agttcacaag agtactctct tccgcgacgg tcgatgtcgt
     2881 ggttgttgat ctagatttag gtcgtgaaga tgggctcgag atcgttcgta atctggcggc
     2941 aaagtctgat attccaatca taattatcag tggcgaccgc cttgaggaga cggataaagt
     3001 tgttgcactc gagctaggag caagtgattt tatcgctaag ccgttcagta tcagagagtt
     3061 tctagcacgc attcgggttg ccttgcgcgt gcgccccaac gttgtccgct ccaaagaccg
     3121 acggtctttt tgttttactg actggacact taatctcagg caacgtcgct tgatgtccga
     3181 agctggcggt gaggtgaaac ttacggcagg tgagttcaat cttctcctcg cgtttttaga
     3241 gaaaccccgc gacgttctat cgcgcgagca acttctcatt gccagtcgag tacgcgacga
     3301 ggaggtttat gacaggagta tagatgttct cattttgagg ctgcgccgca aacttgaggc
     3361 ggatccgtca agccctcaac tgataaaaac agcaagaggt gccggttatt tctttgacgc
     3421 ggacgtgcag gtttcgcacg gggggacgat ggcagcctga gccaattgca tttgcctctt
     3481 aattatctgg ctcaaagggt gactgaggag taagcgatgt gcccatcaca ctgaccacca
     3541 agacgggggt tcgttttgaa gatcaccctt cagacccaag gccagtgtgc gttgatcgcg
     3601 gccacactgc aaattcacat actgtcgata aaggggcgtt ggacgagaac ccattcgtca
     3661 ggatactggc tcgtgatctc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4321 cgcggccttt ttacggttcc tgctcggatc tgttggaccg gacagtagtc atggttgatg
     4381 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     4441 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     4501 tgcggacgtt tttaatgtac tggggttgaa cactctgtgc cttgtcttcc agtggtatgg
     4561 ggtaccgcac ggtctgccaa tccgacggag gtttatgaat tttccacctt ttccacaagc
     4621 tcaactagta ttaacgatgt ggatttagca aaaaaaacct gtaaccctaa atgtaaaata
     4681 acgggtaagc ctgccaacca tgttatggca gattaagcgt ctttttgaag ggcaccgcat
     4741 ctttcgcgtt gccgtaaatg tatccgttta gcaataagga aattcttaat gaataatccg
     4801 tcgttactca atcatgcggt cgaaacgatg gcagttggct cgaaaagttt tgcgacagcc
     4861 tcaaagttat ttgatgcaaa aacccggcgc agcgtactga tgctctacgc ctggtgccgc
     4921 cattgtgacg atgttattga cgatcagacg ctgggctttc aggcccggca gcctgcctta
     4981 caaacgcccg aacaacgtct gatgcaactt gagatgaaaa cgcgccaggc ctatgcagga
     5041 tcgcagatgc acgaaccggc gtttgcggct tttcgggaag tggctatggc tcatgatatc
     5101 gccccggctt acgcgtttga tcatctggaa ggcttcgcca tggatgtacg cgaagcgcaa
     5161 tacagccaac tggatgatac gctgcgctat tgctatcacg ttgcaggcgt tgtcggcttg
     5221 atgatggcgc aaatcatggg cgtgcgggat aacgccacgc tggaccgcgc ctgtgacctt
     5281 gggctggcat ttcagttgac caatattgct cgcgatattg tggacgatgc gcatgcgggc
     5341 cgctgttatc tgccggcaag ctggctggag catgaaggtc tgaacaaaga gaattatgcg
     5401 gcacctgaaa accgtcaggc gctgagccgt atcgcccgtc gtttggtgca ggaagcagaa
     5461 ccttactatt tgtctgccac agccggcctg gcagggttgc ccctgcgttc cgcctgggca
     5521 atcgctacgg cgaagcaggt ttaccggaaa ataggtgtca aagttgaaca ggccggtcag
     5581 caagcctggg atcagcggca gtcaacgacc acgcccgaaa aattaacgct gctgctggcc
     5641 gcctctggtc aggcccttac ttcccggatg cgggctcatc ctccccgccc tgcgcatctc
     5701 tggcagcgcc cgctctaggc ttactataag gatgtttgca atgaaaccaa ctacggtaat
     5761 tggtgcaggc ttcggtggcc tggcactggc aattcgtcta caagctgcgg ggatccccgt
     5821 cttactgctt gaacaacgtg ataaacccgg cggtcgggct tatgtctacg aggatcaggg
     5881 gtttaccttt gatgcaggcc cgacggttat caccgatccc agtgccattg aagaactgtt
     5941 tgcactggca ggaaaacagt taaaagagta tgtcgaactg ctgccggtta cgccgtttta
     6001 ccgcctgtgt tgggagtcag ggaaggtctt taattacgat aacgatcaaa cccggctcga
     6061 agcgcagatt cagcagttta atccccgcga tgtcgaaggt tatcgtcagt ttctggacta
     6121 ttcacgcgcg gtgtttaaag aaggctatct aaagctcggt actgtccctt ttttatcgtt
     6181 cagagacatg cttcgcgccg cacctcaact ggcgaaactg caggcatgga gaagcgttta
     6241 cagtaaggtt gccagttaca tcgaagatga acatctgcgc caggcgtttt ctttccactc
     6301 gctgttggtg ggcggcaatc ccttcgccac ctcatccatt tatacgttga tacacgcgct
     6361 ggagcgtgag tggggcgtct ggtttccgcg tggcggcacc ggcgcattag ttcaggggat
     6421 gataaagctg tttcaggatc tgggtggcga agtcgtgtta aacgccagag tcagccatat
     6481 ggaaacgaca ggaaacaaga ttgaagccgt gcatttagag gacggtcgca ggttcctgac
     6541 gcaagccgtc gcgtcaaatg cagatgtggt tcatacctat cgcgacctgt taagccagca
     6601 ccctgccgcg gttaagcagt ccaacaaact gcagactaag cgcatgagta actctctgtt
     6661 tgtgctctat tttggtttga atcaccatca tgatcagctc gcgcatcaca cggtttgttt
     6721 cggcccgcgt taccgcgagc tgattgacga aatttttaat catgatggcc tcgcagagga
     6781 cttctcactt tatctgcacg cgccctgtgt cacggattcg tcactggcgc ctgaaggttg
     6841 cggcagttac tatgtgttgg cgccggtgcc gcatttaggc accgcgaacc tcgactggac
     6901 ggttgagggg ccaaaactac gcgaccgtat ttttgcgtac cttgagcagc attacatgcc
     6961 tggcttacgg agtcagctgg tcacgcaccg gatgtttacg ccgtttgatt ttcgcgacca
     7021 gcttaatgcc tatcatggct cagccttttc tgtggagccc gttcttaccc agagcgcctg
     7081 gtttcggccg cataaccgcg ataaaaccat tactaatctc tacctggtcg gcgcaggcac
     7141 gcatcccggc gcaggcattc ctggcgtcat cggctcggca aaagcgacag caggtttgat
     7201 gctggaggat ctgatatgag cttttaccca ggagacagag aatgcaaccg cattatgatc
     7261 tgattctcgt gggggctgga ctcgcgaatg gccttatcgc cctgcgactt cagcagcagc
     7321 aacctgatat gcgtattttg cttatcgacg ccgcacccca ggcgggcggg aatcatacgt
     7381 ggtcatttca ccacgatgat ttgactgaga gccaacatcg ttggatagct ccgctggtgg
     7441 ttcatcactg gcccgactat caggtacgct ttcccacacg ccgtcgtaag ctgaacagcg
     7501 gctacttttg tattacttct cagcgtttcg ctgaggtttt acagcgacag tttggcccgc
     7561 acttgtggat ggataccgcg gtcgcagagg ttaatgcgga atctgttcgg ttgaaaaagg
     7621 gtcaggttat cggtgcccgc gcggtgattg acgggcgggg ttatgcggca aattcagcac
     7681 tgagcgtggg cttccaggcg tttattggcc aggaatggcg attgagtcac ccgcatggtt
     7741 tatcatctcc gattatcatg gatgccacgg tcgatcagca aaatggttat cgcttcgtgt
     7801 acagcctgcc gctctcgccg accagattgt taattgaaga tacgcactat attgataatg
     7861 cgacattaga tcctgaatgc gcgcggcaaa atatttgcga ctatgccgcg caacagggtt
     7921 ggcagcttca gacactgctg cgagaagaac agggcgcctt acccattact ctgtcgggca
     7981 atgccgacgc attctggcag cagcgccccc tggcctgtag tggattacgt gccggtctgt
     8041 tccatcctac caccggctat tcactgccgc tggcggttgc cgtggccgac cgcctgagtg
     8101 cacttgatgt ctttacgtcg gcctcaattc accatgccat tacgcatttt gcccgcgagc
     8161 gctggcagca gcagggcttt ttccgcatgc tgaatcgcat gctgttttta gccggacccg
     8221 ccgattcacg ctggcgggtt atgcagcgtt tttatggttt acctgaagat ttaattgccc
     8281 gtttttatgc gggaaaactc acgctgaccg atcggctacg tattctgagc ggcaagccgc
     8341 ctgttccggt attagcagca ttgcaagcca ttatgacgac tcatcgttaa gcttcagagg
     8401 aggaattaag taatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc
     8461 acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc
     8521 cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg
     8581 gtcgaactga ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg
     8641 gatgtggggc atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc
     8701 accatccgtc agaaaggcgg tctgcacccg ttcccgtggc gcggcgaaag cgaatatgac
     8761 gtattaagcg tcgggcattc atcaacctcc attagtgccg gaattggtat tgcggttgct
     8821 gccgaaaaag aaggcaaaaa tcgccgcacc gtctgtgtca ttggcgatgg cgcgattacc
     8881 gcaggcatgg cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg
     8941 attctcaacg acaatgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa caaccatctg
     9001 gcacagctgc tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc
     9061 tctggcgtgc cgccaattaa agagctgctc aaacgcaccg aagaacatat taaaggcatg
     9121 gtagtgcctg gcacgttgtt tgaggagctg ggctttaact acatcggccc ggtggacggt
     9181 catgatgtgc tggggcttat caccacgcta aagaacatgc gcgacctgaa aggcccgcag
     9241 ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agacccgatc
     9301 actttccatg ccgtgctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg
     9361 gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca
     9421 acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac
     9481 gtaaattccc ggatcgctac ttcgacgtgg caattgccga gcaacatgcg gtgacctttg
     9541 ctgcgggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc
     9601 aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagcttccg gtcctgttcg
     9661 ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggt gcttttgatc
     9721 tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat
     9781 gtcgccagat gctctatacc ggctatcact ataacgatgg cccgtcagcg gtgcgctacc
     9841 cgcgtggcaa cgcggtcggc gtggaactga cgccgctgga aaaactacca attggcaaag
     9901 gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag
     9961 aagcggcgaa agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt tttgtgaaac
    10021 cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag
    10081 aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgctg atggcccatc
    10141 gtaaaccagt acccgtgctg aacattggcc tgccggactt ctttattccg caaggaactc
    10201 aggaagaaat gcgcgccgaa ctcggcctcg atgccgctgg tatggaagcc aaaatcaagg
    10261 cctggctggc ataagctttg tgtgaggaga tgtcgaatga gcgcacatgc cctgcccaag
    10321 gcagatctga ccgccaccag cctgatcgta tcgggcggca tcatcgccgc ttggctggcc
    10381 ctgcatgtgc atgcgctgtg gtttctggac gcagcggcgc atcccatcct ggcgatcgca
    10441 aatttcctgg ggctgacctg gctgtcggtc ggattgttca tcatcgcgca tgacgcgatg
    10501 cacgggtcgg tggtgccggg gcgtccgcgc gccaatgcgg cgatgggcca gcttgtcctg
    10561 tggctgtatg ccggattttc gtggcgcaag atgatcgtca agcacatggc ccatcaccgc
    10621 catgccggaa ccgacgacga ccccgatttc gaccatggcg gcccggtccg ctggtacgcc
    10681 cgcttcatcg gcacctattt cggctggcgc gaggggctgc ttctgcccgt catcgtgacg
    10741 gtctatgcgc tgatccttgg ggatcgctgg atgtacgtgg tattctggcc gctgccgtcg
    10801 atcctggcgt cgatccagct gttcgtgttc ggcacctggc tgccgcaccg ccccggccac
    10861 gacgcgttcc cggaccgcca caatgcgcgg tcgtcgcgga tcagcgaccc cgtgtcgctg
    10921 ctgacctgtt ttcactttgg cggttatcat cacgaacacc acctgcaccc gacggtgccg
    10981 tggtggcgcc tgcccagcac ccgcaccaag ggcgacaccg cataagcttg agctgaggag
    11041 attttgaatg caaacggaac acgtcatttt attgaatgca cagggagttc ccacgggtac
    11101 gctggaaaag tatgccgcac acacggcaga cacccgctta catctcgcgt tctccagttg
    11161 gctgtttaat gccaaaggac aattattagt tacccgccgc gcactgagca aaaaagcatg
    11221 gcctggcgtg tggactaact cggtttgtgg gcacccacaa ctgggagaaa gcaacgagga
    11281 cgcagtgatc cgccgttgcc gttatgagct tggcgtggaa attacgcctc ctgaatctat
    11341 ctatcctgac tttcgctacc gcgccaccga tccgagtggc attgtggaaa atgaagtgtg
    11401 tccggtattt gccgcacgca ccactagtgc gttacagatc aatgatgatg aagtgatgga
    11461 ttatcaatgg tgtgatttag cagatgtatt acacggtatt gatgcaacgc cgtgggcgtt
    11521 cagtccgtgg atggtgatgc aggcgacaaa tcgcgaagcc agaaaacgat tatctgcatt
    11581 tacccagctt aaataagctt tgcctcagga tttttgaaat gacggtctgc gcaaaaaaac
    11641 acgttcatct cactcgcgat gctgcggagc agttactggc tgatattgat cgacgccttg
    11701 atcagttatt gcccgtggag ggagaacggg atgttgtggg tgccgcgatg cgtgaaggtg
    11761 cgctggcacc gggaaaacgt attcgcccca tgttgctgtt gctgaccgcc cgcgatctgg
    11821 gttgcgctgt cagccatgac ggattactgg atttggcctg tgcggtggaa atggtccacg
    11881 cggcttcgct gatccttgac gatatgccct gcatggacga tgcgaagctg cggcgcggac
    11941 gccctaccat tcattctcat tacggagagc atgtggcaat actggcggcg gttgccttgc
    12001 tgagtaaagc ctttggcgta attgccgatg cagatggcct cacgccgctg gcaaaaaatc
    12061 gggcggtttc tgaactgtca aacgccatcg gcatgcaagg attggttcag ggtcagttca
    12121 aggatctgtc tgaaggggat aagccgcgca gcgctgaagc tattttgatg acgaatcact
    12181 ttaaaaccag cacgctgttt tgtgcctcga tgcagatggc ctcgattgtt gcgaatgcct
    12241 ccagcgaagc gcgtgattgc ctgcatcgtt tttcacttga tcttggtcag gcatttcaac
    12301 tgctggacga tttgaccgat ggcatgaccg acaccggtaa ggatagcaat caggacgccg
    12361 gtaaatcgac gctggtcaat ctgttaggcc cgagggcggt tgaagaacgt ctgagacaac
    12421 atcttcagct tgccagtgag catctctctg cggcctgcca acacgggcac gccactcaac
    12481 attttattca ggcctggttt gacaaaaaac tcgctgccgt cagttaagct tgcaagaatt
    12541 caagcttgga gtacgttaat tagttgtctg ttttaagccc cgtgctctcc gccggggctt
    12601 ttttattaaa aaccactcaa gtggaagaca agggacacga agtgatccgt ttaaactatc
    12661 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
    12721 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccagccg
    12781 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
    12841 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
    12901 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
    12961 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
    13021 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
    13081 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
    13141 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
    13201 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
    13261 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
    13321 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
    13381 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
    13441 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
    13501 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
    13561 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
    13621 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
    13681 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
    13741 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
    13801 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
    13861 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
    13921 cgtggaa
//
