LOCUS       Exported                8301 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral vector (pRRL) containing Her2-ITD specific T cell 
            receptor.
ACCESSION   .
VERSION     .
KEYWORDS    pJV137
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Veatch JR, Jesernig BL, Kargl J, Fitzgibbon M, Lee SM, Baik C, 
            Martins R, Houghton AM, Riddell SR
  TITLE     Endogenous CD4(+) T Cells Recognize Neoantigens in Lung Cancer 
            Patients, Including Recurrent Oncogenic KRAS and ERBB2 (Her2) Driver
            Mutations.
  JOURNAL   Cancer Immunol Res. 2019 Jun;7(6):910-922. doi: 
            10.1158/2326-6066.CIR-18-0402. Epub 2019 May 1.
  PUBMED    31043415
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1325..1381
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2207..2795
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2260..2280)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2678..2689)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2882..3115
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3187..3308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3224..3243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3278..3297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3348..3483
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3410..3429
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3503..3522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3504..3522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3532..3549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3532..3548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3541..3563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3690..4145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3777..3796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3987..4008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4171..4275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4276..5136
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4494..4513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5307..5895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5796..5815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6049..6066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6183..6204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6219..6249
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6257..6273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6262..6284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6281..6297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6281..6297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6316..6336
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6318..6336
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6364..6590
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6591..6771
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6818..6943
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7436..7669
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7854..7898
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8165..8282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgagggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
       61 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
      121 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      181 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
      241 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      301 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      361 ccacaacccc tcactcggcg cgccggccac catgttgctt ttgttgctgc tcctcggacc
      421 tggctctgga ctgggagctg tggtttctca gcacccctct tgggtcatct gcaagagcgg
      481 caccagcgtg aagatcgagt gcagaagcct ggacttccag gccaccacaa tgttctggta
      541 cagacagttc cccaagcaga gcctgatgct gatggccacc tctaacgagg gcagcaaggc
      601 cacatatgag cagggcgtcg agaaggacaa gttcctgatc aaccacgcca gcctgacact
      661 gagcaccctg acagtgacaa gcgcccatcc tgaggacagc agcttctaca tctgtagcgc
      721 ccctccactg gccggcgacg agacacaata ttttggccct ggcaccagac tgctggtgct
      781 ggacctgaag aacgtgttcc cacctgaggt ggccgtgttc gagccttctg aggccgagat
      841 cagccacaca cagaaagcca cactcgtgtg tctggccacc ggcttctatc ccgatcacgt
      901 cgaactgtct tggtgggtca acggcaaaga ggtgcacagc ggcgtctgta ccgatcctca
      961 gcctctgaaa gagcagcccg ctctgaacga cagcagatac tgcctgagca gcagactgag
     1021 agtgtccgcc accttctggc agaaccccag aaaccacttc agatgccagg tgcagttcta
     1081 cggcctgagc gagaacgatg agtggaccca ggatagagcc aagcctgtga cacagatcgt
     1141 gtctgccgaa gcctggggca gagccgattg tggctttacc agcgagagct accagcaagg
     1201 cgtgctgtct gccaccatcc tgtacgagat cctgctgggc aaagccactc tgtacgccgt
     1261 gctggtgtct gccctggtcc tgatggctat ggtcaagcgg aaggatagca gaggcggaag
     1321 cggcgccaca aacttctcac tgctgaaaca ggcaggcgac gtggaagaga accccggacc
     1381 tatgctgctg cttctggtgc ctgccttcca agtgatcttt accctcggcg gcacaagagc
     1441 ccagagcgtg acacagctgg atagccaggt gccagtgttc gaagaggccc ctgtggaact
     1501 gcggtgcaac tacagcagca gcgtgtccgt gtacctgttt tggtacgtgc agtaccccaa
     1561 ccagggcctg cagctgctcc tgaagtatct gagcggcagc accctggtgg aatccatcaa
     1621 tggcttcgag gccgaattca acaagagcca gaccagcttc cacctgagaa agcccagcgt
     1681 gcacatcagc gataccgccg agtacttctg cgccgtgtct gtgaacaccg acaagctgat
     1741 cttcggcacc ggcacaaggc tccaggtgtt ccccaacatt cagaaccccg atcctgcagt
     1801 gtaccagctg cgggacagca agagcagcga caagagcgtg tgcctgttca ccgacttcga
     1861 cagccagacc aacgtgtccc agagcaagga cagcgacgtg tacatcaccg ataagtgcgt
     1921 gctggacatg cggagcatgg acttcaagag caacagcgcc gtggcctggt ccaacaagag
     1981 cgacttcgcc tgcgccaacg ccttcaacaa cagcattatc cccgaggaca cattcttccc
     2041 aagccccgag agcagctgcg acgtgaagct ggtggaaaag agcttcgaga cagacaccaa
     2101 cctgaacttc cagaacctca gcgtgatcgg cttccggatc ctgctgctga aggtggccgg
     2161 cttcaacctg ctgatgaccc tgcggctgtg gtccagctga gtcgacaatc aacctctgga
     2221 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2281 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2341 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2401 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2461 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2521 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2581 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     2641 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2701 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2761 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     2821 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     2881 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     2941 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3001 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3061 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     3121 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     3181 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3301 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3361 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3421 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3481 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3541 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3601 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3661 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3841 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3901 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4021 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4081 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4141 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6061 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6301 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6361 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6421 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     6481 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     6541 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     6601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6781 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6841 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6901 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6961 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7021 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     7081 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7141 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7201 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7261 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7321 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7381 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7441 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7501 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7561 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7621 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7681 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7741 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7801 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7861 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7921 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7981 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     8041 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     8101 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     8161 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8221 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8281 ttatcgatca cgagactagc c
//
