LOCUS       Exported                6881 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Zebrafish Tol1 construct for UAS-mediated expression of the 
            optogenetic opsin GtACR1 fused to the red fluorescent protein 
            tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    pTol1-UAS:GtACR1-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Antinucci P, Dumitrescu A, Deleuze C, Morley HJ, Leung K, Hagley T, 
            Kubo F, Baier H, Bianco IH, Wyart C
  TITLE     A calibrated optogenetic toolbox of stable zebrafish opsin lines.
  JOURNAL   Elife. 2020 Mar 27;9. pii: 54937. doi: 10.7554/eLife.54937.
  PUBMED    32216873
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4485..4502)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4485..4501)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4494..4516)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4710..4729)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4829..4851
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4889..4907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4975..5079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5080..5940
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5298..5317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6111..6699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6600..6619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6853..6870
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg
      301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact
      361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag
      421 gcctccaagg cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg
      481 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg
      541 tcctccgggc tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag
      601 aggtcggagt actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg
      661 gagtactgtc ctccgacgca aggtcggagt actgtcctcc gacactagag gtcggagtac
      721 tgtcctccga cgcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca
      781 agggtcgact ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc
      841 ctccacacga attcgatact aaggatcctt gtttgtgagt atgatgttaa atataagcaa
      901 tcgacgtttg ttttgtcgtt tgttaaatct agcaatgttt tatttcaatt aaagtgttaa
      961 ccggttcatt taaaggtgta tttctgaatg agctaacgta ttagctttta acgctagccg
     1021 gactgtttgg ttttgaggat caataaacca acgttttttt ctgatttatt ttgctttaaa
     1081 atataaaggt ataattcgtc gtcgagaatt ttgggttttg ttcgagttta gaaatttggc
     1141 ggcgtttttt tgtaccgcga aacagggcgg tggttcggtg agcttctcaa ttcatctgct
     1201 tgacaaacgg ttttcggcaa ctgtaaatgc gctatgatgc ttttattctt ttcagcgtgt
     1261 aactttgtta aatataattg actttccgac tatcgacgtt cgtttcatct ccgtgttatt
     1321 gtttaatggc cattttctgt ggtaaactgt cgccattttg ttttcagtcg tccgtttgtt
     1381 ccgcccttac tcacagctgt gtcagcatta gtgatgactg ttctcacctg gcctgtttgc
     1441 tttcattatt gatgaagagc tttctagaga attctgaagc tcgtctttgt cctgaattca
     1501 tctgtattca tttaaaaaaa aaaattaacc ttaatttttt tttcttctca gcgtaaagcc
     1561 accatgagca gcatcacctg tgatcccgcc atctacggcg aatggagcag ggagaaccag
     1621 ttctgcgtgg agaagagcct gatcaccctg gacggcatca agtacgtcca gctggtgatg
     1681 gccgtcgtga gcgcctgtca ggtgttcttc atggtgacca gagcccccaa ggtgccctgg
     1741 gaagccatct acctgcccac caccgagatg atcacctatt ccctggcctt caccggaaac
     1801 ggctacatca gagtggccaa tggcaagtac ctgccctggg ccagaatggc cagctggctg
     1861 tgcacctgcc ctatcatgct gggcctggtg agcaatatgg ccctcgtgaa gtacaagagc
     1921 atccccctca accctatgat gatcgccgcc tccagcatct gcaccgtgtt cggcatcacc
     1981 gcctccgtgg tgctagaccc gctgcacgtg tggctgtact gtttcatcag cagcatcttc
     2041 ttcatcttcg agatggtggt ggccttcgcc attttcgcca ttaccatcca cgatttccag
     2101 accatcggct cccccatgtc cctgaaggtg gtggagaggc tgaagctgat gaggatcgtg
     2161 ttctacgtga gctggatggc ctaccctatc ctgtggagct tctccagcac cggcgcctgc
     2221 atcatgagcg agaacaccag cagcgtgctg tacctgctgg gcgacgctct gtgcaagaac
     2281 acctacggca tcctgctgtg ggctacaacc tggggcctgc tgaacggcaa gtgggacagg
     2341 gattacgtga agggcaggaa cgtggacggc accctgatgc ctgagtacga gcaggacctg
     2401 gagaagggca acaccgagag gtacgaggac gccagagccg gcgagaccgc ggcaccggta
     2461 gtagcagtga gtaagggcga ggaagtgatc aaagagttca tgcggtttaa ggtgagaatg
     2521 gaaggaagca tgaacggcca cgagttcgaa attgagggag aaggagaggg acggccctac
     2581 gagggcaccc agacagccaa gctgaaagtg acaaagggcg ggcctctgcc attcgcttgg
     2641 gacatcctga gcccacagtt tatgtacggc tccaaggcct atgtgaaaca tccagctgac
     2701 attcccgatt ataagaaact gagcttcccc gaggggttta agtgggaaag agtgatgaac
     2761 ttcgaggacg gaggcctggt gactgtgacc caggacagct ccctgcagga tgggaccctg
     2821 atctacaagg tgaaaatgag agggacaaat tttccccctg atggacctgt gatgcagaag
     2881 aaaactatgg gatgggaggc ctccaccgaa aggctgtatc cacgcgacgg ggtgctgaaa
     2941 ggagaaatcc accaggctct gaagctgaaa gatgggggac attacctggt ggagttcaag
     3001 acaatctaca tggccaagaa acctgtgcag ctgccaggct actattacgt ggacacaaaa
     3061 ctggatatca cttcacacaa cgaggactac actattgtgg agcagtatga acggagcgag
     3121 gggagacacc atctgttcct gggccatggg actggaagta ccggctcagg gtctagtgga
     3181 accgcctcaa gcgaggataa caatatggct gtgatcaaag agttcatgag gtttaaggtg
     3241 cgcatggagg gcagcatgaa tgggcacgaa tttgagattg aaggagaggg cgaagggagg
     3301 ccttacgagg gcacacagac tgccaagctg aaagtgacca agggaggacc actgcctttc
     3361 gcttgggata tcctgtctcc tcagtttatg tacggaagta aggcctatgt caagcatccc
     3421 gctgacattc ctgattacaa gaaactgtct ttcccagagg gctttaagtg ggagagagtg
     3481 atgaattttg aagatggagg cctggtgacc gtgacacagg actcctctct gcaggatggc
     3541 actctgatct acaaagtcaa aatgcgcggc accaattttc cacccgatgg gcccgtgatg
     3601 cagaagaaaa caatggggtg ggaggccagc actgaacggc tgtatcctag agacggagtg
     3661 ctgaagggcg aaatccacca ggccctgaag ctgaaagacg gcggccacta cctggtggag
     3721 ttcaaaacca tctacatggc caagaaacca gtgcagctgc ccggctatta ctatgtggac
     3781 accaagctgg atatcacatc ccacaatgaa gactacacca ttgtggaaca gtatgagagg
     3841 tctgaaggac gccaccatct gtttctgtac ggcatggatg agctgtataa gtaaacatgg
     3901 agatctcgag actagtaatt aagtctcagc caccgttaac tgaacatgtc aaaacctgtg
     3961 gagactgttg agatttgatg ttctgaaaag ataaagccta taaataaaat gttgcccaaa
     4021 tttcctgcct gatgtttttc tttgtctttg ctacatggct ttgctgctcg gatcggctca
     4081 ctctgtgtat gccacgttca ctttgtactc tccttctcac ggtaggttta ttatttttag
     4141 atgtgcagtt agtttctgtg aaataacaca ccacacactg atattgtctg tgcattgact
     4201 tggtgagtgc acattgtttt tgatcttgac atatttatat ttgattgatc aggtgaactg
     4261 tgtgaatcta aagtgctcca tacagatgtt ctgcattgaa aatattctca ttttattagt
     4321 ggaagtgagt gtatgccagc ggccgcggta ccctgcagtc tagacatttc agttgacgaa
     4381 gacaaacaaa gttctgttgt gactatgggg ggggggggcg cctggggatg gtctcgcccg
     4441 gggagtaatt cagggtagaa ccgccactgc ctttagcgac attcactggc cgtcgtttta
     4501 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4561 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4621 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4681 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4741 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4801 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4861 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5761 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//
