LOCUS       Exported               11314 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of
            Dox controlled H2Bneon.
ACCESSION   .
VERSION     .
KEYWORDS    pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Tejero R, Huang Y, Katsyv I, Kluge M, Lin JY, Tome-Garcia J, Daviaud
            N, Wang Y, Zhang B, Tsankova NM, Friedel CC, Zou H, Friedel RH
  TITLE     Gene signatures of quiescent glioblastoma cells reveal mesenchymal 
            shift and interactions with niche microenvironment.
  JOURNAL   EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 
            10.1016/j.ebiom.2019.03.064.
  PUBMED    30952620
REFERENCE   2  (bases 1 to 11314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             961..1764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        2334..3544
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3381..3400
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3587..4330
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    4340..4474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4364..4383)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    4825..4906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5053..5763)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      5499..5508
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      5760..5769
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(5782..6159)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(6188..6502)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(6191..6215)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6262..6280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6298..6316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6333..6351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6369..6387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6405..6423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6440..6458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6476..6494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6550..7386
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7420..7439)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7421..7439)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7446..7463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7446..7462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7600..7902
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7781..7800
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9299..10159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9922..9941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10283..10871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10772..10791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11025..11042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11159..11180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11195..11225
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11233..11249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11238..11260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11257..11273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11257..11273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11294..11312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagacgcgt
      961 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1021 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1081 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1141 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1201 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1261 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1321 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1381 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1441 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1501 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1561 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1621 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1681 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1741 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct
     1801 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
     1861 ggtgagaaca gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt
     1921 gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
     1981 gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt atgataatgt
     2041 ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
     2101 cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc
     2161 ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac
     2221 gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta
     2281 attccatcag aagctgggag ctccaccgcg gtggcggccg ctctagaact agtgcctccg
     2341 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2401 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2461 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2521 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2581 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2641 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2701 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2761 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2821 gtagtctggg tccgcgagca aggttgccct gaactggggg ttccggggag cgcagcaaaa
     2881 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2941 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     3001 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     3061 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     3121 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3181 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3241 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3301 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3361 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3421 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3481 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3541 agaccccggg ctgcaggaat tcaccatgcc aaagagaccc agaccctcta gactggacaa
     3601 gagcaaagtc ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac
     3661 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     3721 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     3781 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     3841 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     3901 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     3961 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     4021 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     4081 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     4141 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     4201 cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     4261 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     4321 ccccgggtaa ctaagtaagg atccagacat gataagatac attgatgagt ttggacaaac
     4381 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4441 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4501 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     4561 tggtatggct gattatgatc ctgcaagcct cgtcgtcctg gccggaccac gctatctgtg
     4621 caaggtcccc ggccccggac gcgcgctcca tgagcagagc gcccgccgcc gaggcgaaga
     4681 ctcgggcggc gccctgcccg tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg
     4741 gaatgcgcgc gaccttcagc atcgccggca tgtccccctg gcggacggga agtatccagc
     4801 tcgaccaagc ttagtactgt cgaggcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4861 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4921 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     4981 cctctacaaa tgtggtatgg ctgattatga tcctctagag atatcgtcga caagcttatc
     5041 gatgcggccg ctttacttgt acagctcgtc catgcccatc acatcggtaa aggccttttg
     5101 ccactccttg aagttgagct cggtcttgga gtgcttgagc tccgtcttac ggaacacgta
     5161 catcggctgg ttcttcagat agttagccgc cattggcttg gcaaaggtgt aggtggtccg
     5221 cgcagtgctc cggtagcgct tgccatttcc agtggtgtaa ctccacttaa aggtactgat
     5281 gatggttttg tcgttggggt aagtcttctt cgacctgcac cagtccgcag cggtcagcga
     5341 gttggtcatc acaggaccgt cagcagggaa accagtcccc ttcacctggg cctctccttt
     5401 gatgtggctt ccctcgtagg tgtagcggta gttaacagta agggaggcac catcttcaaa
     5461 ctgcattgtg cgatggactt ggtatccgga gccatctacc atggcggcct ggaaaggcga
     5521 catcccgtca gggtagggca ggtactgatg gaagccatac ccgatatgag ggaccagaat
     5581 ccagggggag aactggaggt cacccttggt ggacttcagg tttaactcct cataaccatc
     5641 atttggattg ccggtgccct gacccaccat gtcaaagtcc acaccgttga tggagccaaa
     5701 gatgtgtaac tcatgtgtcg ctgggagaga ggccatgtta tcctcctcgc ccttgctcac
     5761 catggtggcg accggtggat ccttagcgct ggtgtacttg gtgatggcct tagtaccctc
     5821 ggacacggcg tgcttggcca actccccagg cagcagcagg cgcacggccg tctggatctc
     5881 cctggaggtg atggtcgagc gcttgttgta atgcgccagg cgggaagcct cacctgcgat
     5941 gcgctcgaaa atgtcgttca caaacgaatt catgatgccc atggccttgg acgaaatgcc
     6001 ggtgtcaggg tggacctgct tcagaacctt gtacacatag atggaatagc tctccttgcg
     6061 gctgcgcttg cgcttcttgc cgcctttctt ctgcgcctta gtcaccgcct tcttggagcc
     6121 ctttttcggg gcgggagcag acttcgctgg ctctggcatg gtggcggtac cgagctcgaa
     6181 ttctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta
     6241 cacgcctacc tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     6301 ctatcactga tagggataaa ctcgacatac gttctctatc actgataggg agtaaactcg
     6361 acatacgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
     6421 ggagtaaact cgacatcgtt ctctatcact gatagggagt aaactcgaca tacgttctct
     6481 atcactgata gggagtaaac tcgacccata gagcccaccg catccccagc atgcctgcta
     6541 ttaagcttta ctagggacag gattggtgac agaaaagccc catccttagg cctcctcctt
     6601 cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc cttatctggt
     6661 gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa ggtttgctta
     6721 cgatggagcc agagaggatc ctgggaggga gagcttggca gggggtggga gggaaggggg
     6781 ggatgcgtga cctgcccggt tctcagtggc caccctgcgc taccctctcc cagaacctga
     6841 gctgctctga cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag cttccttaca
     6901 cttcccaaga ggagaagcag tttggaaaaa caaaatcaga ataagttggt cctgagttct
     6961 aactttggct cttcaccttt ctagtcccca atttatattg ttcctccgtg cgtcagtttt
     7021 acctgtgaga taaggccagt agccagcccc gtcctggcag ggctgtggtg aggagggggg
     7081 tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg
     7141 gccgcctcta ctccctttct ctttctccat ccttctttcc ttaaagagtc cccagtgcta
     7201 tctgggacat attcctccgc ccagagcagg gtcccgcttc cctaaggccc tgctctgggc
     7261 ttctgggttt gagtccttgg caagcccagg agaggcgctc aggcttccct gtcccccttc
     7321 ctcgtccacc atctcatgcc cctggctctc ctgccccttc cctacagggg ttcctggctc
     7381 tgctctaagg gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga gtcgtattac
     7441 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7561 gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat
     7621 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg
     7681 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     7741 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     7801 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     7861 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta
     7921 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
     7981 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
     8041 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
     8101 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     8161 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
     8221 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     8281 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8341 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8401 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     8461 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     8521 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     8581 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     8641 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     8701 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     8761 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatgcatgg
     8821 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     8881 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     8941 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9001 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc
     9061 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     9121 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9181 attcaaatat gtatccgctc atgagattat caaaaaggat cttcacctag atccttttaa
     9241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9601 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9841 tgactggtga atactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9901 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10201 attgtctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10921 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10981 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11041 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11101 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11161 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11221 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11281 acgccaagct cagaattaac cctcactaaa ggga
//
