LOCUS Exported 10548 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of CXXC domain only form of TET1. ACCESSION . VERSION . KEYWORDS pEF1a_ TET1 CXXC only SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10548) AUTHORS Vincenzetti L, Leoni C, Chirichella M, Kwee I, Monticelli S TITLE The contribution of active and passive mechanisms of 5mC and 5hmC removal in human T lymphocytes is differentiation- and activation-dependent. JOURNAL Eur J Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967. PUBMED 30698829 REFERENCE 2 (bases 1 to 10548) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10548 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(45..64) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 352..1530 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 583..1521 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1478..1498 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 1547..1566 /label=T7 /note="T7 promoter, forward primer" promoter 1547..1565 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 1598..1607 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1607..1630 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1643..1669 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 6058..6099 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 6109..6126 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(6149..6166) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6155..6379 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6425..6853 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6512..6531) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6722..6743 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6862..6882) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6867..7196 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7047..7182 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7109..7128 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7263..8057 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7317..7336) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7927..7946 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 8233..8354 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8270..8289) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8324..8343 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8403..8419) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8403..8419) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8416..8438) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8427..8443 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8451..8481) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8496..8517 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8634..8651) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8794..9382) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8874..8893) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9553..10413) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10176..10195 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10414..10518) /gene="bla" /label=AmpR promoter ORIGIN 1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaagacaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag 301 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt 361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 421 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt 601 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc 661 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg 721 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc 781 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag 841 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt 901 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc 961 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca 1021 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg 1081 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga 1141 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc 1201 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc 1261 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg 1321 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc 1381 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg 1441 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa 1501 agtttttttc ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact 1561 atagggagac ccaagctggc taggtaagct tggtaccgcc accatggact acaaggacga 1621 cgatgacaag ctcgatggag gataccccta cgacgtgccc gactacgccg gaggactcga 1681 ctctcgatcc cgccatgcaa ggccttccag attagtcagg aaggaagatg taaacaaaaa 1741 aaagaaaaac agccaactac gaaagacaac caagggagcc aacaaaaatg tggcatcagt 1801 caagacttta agccctggaa aattaaagca attaattcaa gaaagagatg ttaagaaaaa 1861 aacagaacct aaaccacccg tgccagtcag aagccttctg acaagagctg gagcagcacg 1921 catgaatttg gataggactg aggttctttt tcagaaccca gagtccttaa cctgcaatgg 1981 gtttacaatg gcgctacgaa gcacctctct tagcaggcga ctctcccaac ccccactggt 2041 cgtagccaaa tccaaaaagg ttccactttc taagggttta gaaaagcaac atgattgtga 2101 ttataagata ctccctgctt tgggagtaaa gcactcagaa aatgattcgg ttccaatgca 2161 agacacccaa gtccttcctg atatagagac tctaattggt gtacaaaatc cctctttact 2221 taaaggtaag agccaagaga caactcagtt ttggtcccaa agagttgagg attccaagat 2281 caatatccct acccacagtg gccctgcagc tgagatcctt cctgggccac tggaagggac 2341 acgctgtggt gaaggactat tctctgaaga gacattgaat gataccagtg gttccccaaa 2401 aatgtttgct caggacacag tgtgtgctcc ttttccccaa agagcaaccc ccaaagttac 2461 ctctcaagga aaccccagca ttcagttaga agagttgggt tcacgagtag aatctcttaa 2521 gttatctgat tcttacctgg atcccattaa aagtgaacat gattgctacc ccacctccag 2581 tcttaataag gttatacctg acttgaacct tagaaactgc ttggctcttg gtgggtctac 2641 gtctcctacc tctgtaataa aattcctctt ggcaggctca aaacaagcga cccttggtgc 2701 taaaccagat catcaagagg ccttcgaagc tactgcaaat caacaggaag tttctgatac 2761 cacctctttc ctaggacagg cctttggtgc tatcccacat caatgggaac ttcctggtgc 2821 tgacccagtt catggtgagg ccctgggtga gaccccagat ctaccagaga ttcctggtgc 2881 tattccagtc caaggagagg tctttggtac tattttagac caacaagaaa ctcttggtat 2941 gagtgggagt gttgtcccag acttgcctgt cttccttcct gttcctccaa atccaattgc 3001 tacctttaat gctccttcca aatggcctga gccccaaagc actgtctcat atggacttgc 3061 agtccagggt gctatacaga ttttgccttt gggctcagga cacactcctc aatcatcatc 3121 aaactcagag aaaaattcat tacctccagt aatggctata agcaatgtag aaaatgagaa 3181 gcaggttcat ataagcttcc tgccagctaa cactcagggg ttcccattag cccctgagag 3241 aggactcttc catgcttcac tgggtatagc ccaactctct caggctggtc ctagcaaatc 3301 agacagaggg agctcccagg tcagtgtaac cagcacagtt catgttgtca acaccacagt 3361 ggtgactatg ccagtgccaa tggtcagtac ctcctcttct tcctatacca ctttgctacc 3421 gactttggaa aagaagaaaa gaaagcgatg tggggtctgt gaaccctgcc agcagaagac 3481 caactgtggt gaatgcactt actgcaagaa cagaaagaac agccatcaga tctgtaagaa 3541 aagaaaatgt gaggagctga aaaagaaacc atctgttgtt gtgcctctgg aggttataaa 3601 ggaaaacaag aggccccaga gggaaaagaa gcccaaagtt ttaaaggcag attttgacaa 3661 caaaccagta aatggcccca agtcagaatc catggactac agtagatgtg gtcatgggga 3721 agaacaaaaa ttggaattga acccacatac tgttgaaaat gtaactaaaa atgaagacag 3781 catgacaggc atcgaggtgg agaagtggac acaaaacaag aaatcacagt taactgatca 3841 cgtgaaagga gattttagtg ctaatgtccc agaagctgaa aaatcgaaaa actctgaagt 3901 tgacaagaaa cgaaccaaat ctccaaaatt gtttgtacaa accgtaagaa atggcattaa 3961 acatgtacac tgtttaccag ctgaaacaaa tgtttcattt aaaaaattca atattgaaga 4021 attcggcaag acattggaaa acaattctta taaattccta aaagacactg caaaccataa 4081 aaacgctatg agctctgttg ctactgatat gagttgtgat catctcaagg ggagaagtaa 4141 cgttttagta ttccagcagc ctggctttaa ctgcagttcc attccacatt cttcacactc 4201 catcataaat catcatgcta gtatacacaa tgaaggtgat caaccaaaaa ctcctgagaa 4261 tataccaagt aaagaaccaa aagatggatc tcccgttcaa ccaagtctct tatcgttaat 4321 gaaagatagg agattaacat tggagcaagt ggtagccata gaggccctga ctcaactctc 4381 agaagcccca tcagagaatt cctccccatc aaagtcagag aaggatgagg aatcagagca 4441 gagaacagcc agtttgctta atagctgcaa agctatcctc tacactgtaa gaaaagacct 4501 ccaagaccca aacttacagg gagagccacc aaaacttaat cactgtccat ctttggaaaa 4561 acaaagttca tgcaacacgg tggttttcaa tgggcaaact actacccttt ccaactcaca 4621 tatcaactca gctactaacc aagcatccac aaagtcacat gaatattcaa aagtcacaaa 4681 ttcattatct ctttttatac caaaatcaaa ttcatccaag attgacacca ataaaagtat 4741 tgctcaaggg ataattactc ttgacaattg ttccaatgat ttgcatcagt tgccaccaag 4801 aaataatgaa gtggagtatt gcaaccagtt actggacagc agcaaaaaat tggactcaga 4861 tgatctatca tgtcaggatg caacccatac ccaaattgag gaagatgttg caacacagtt 4921 gacacaactt gcttcgataa ttaagatcaa ttatataaaa ccagaggaca aaaaagttga 4981 aagtacacca acaagccttg tcacatgtaa tgtacagcaa aaatacaatc aggagaaggg 5041 cacaatgcaa cagaaaccac cttcaagtgt acacaataat catggttcat cattaacaaa 5101 acaaaagaac ccaacccaga aaaagacaaa atccacccca tcaagagatc ggcggaaaaa 5161 gaagcccaca gttgtaagtt atcaagaaaa tgatcggcag aagtgggaaa agttgtccta 5221 tatgtatggc acaatatgcg acatttggat agcatcgaaa tttcaaaatt ttgggcaatt 5281 ttgtccacat gattttccta ctgtatttgg gaaaatttct tcctcgacca aaatatggaa 5341 accactggct caaacgaggt ccattatgca acccaaaaca gtatttccac cactcactca 5401 gataaaatta cagagatatc ctgaatcagc agaggaaaag gtgaaggttg aaccattgga 5461 ttcactcagc ttatttcatc ttaaaacgga atccaacggg aaggcattca ctgataaagc 5521 ttataattct caggtacagt taacggtgaa tgccaatcag aaagcccatc ctttgaccca 5581 gccctcctct ccacctaacc agtgtgctaa cgtgatggca ggcgatgacc aaatacggtt 5641 tcagcaggtt gttaaggagc aactcatgca tcagagactg ccaacattgc ctggtatctc 5701 tcatgaaaca cccttaccgg agtcagcact aactctcagg aatgtaaatg tagtgtgttc 5761 aggtggaatt acagtggttt ctaccaaaag tgaagaggaa gtctgttcat ccagttttgg 5821 aacatcagaa ttttccacag tggacagtgc acagaaaaat tttaatgatt atgccatgaa 5881 cttctttact aaccctacaa aaaacctagt gtctattaat taaatgaatt gaaccaaatt 5941 ccttctcata aagcattaac attaacccat gacaatgttg tcaccgtgtc cccttatgct 6001 ctcacacacg ttgcggggcc ctataaccat tgggtctgat ctagagggcc cttcgaaggt 6061 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat 6121 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 6181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 6241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 6301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 6361 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 6421 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 6481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 6541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 6601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 6661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 6721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 6781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 6841 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 6901 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 6961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 7021 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 7081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 7141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 7201 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc 7261 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 7321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 7381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 7441 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 7501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 7561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 7621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 7681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 7741 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 7801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 7861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 7921 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 7981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 8041 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc 8101 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 8161 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 8221 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8281 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 8341 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 8401 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8461 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 8521 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8581 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 8641 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8701 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 8821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 8881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 8941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 9001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 9061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 9121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 9181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 9241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 9721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 9781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 9841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 9901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 10021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 10081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 10141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 10201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 10261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt //