LOCUS       Exported                3583 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  yeast plasmid overexpressing LovC.
ACCESSION   .
VERSION     .
KEYWORDS    pZ_lacO-cP-AOX1-LovC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3583)
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    83..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1377..1394
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1474..1720
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1735..2146
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2154..2201
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2220..2594
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2660..2907
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2676..2694)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3062..3081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
       61 tgagatcaga tcttgtgtgg aattgtgagc ggataacaat ttcacacact aacccctact
      121 tgacagcaat atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca
      181 ttattagctt actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga
      241 cttttaacga caacttgaga agatcaaaaa acaactaatt attcgaaatg ggcgaccagc
      301 cattcattcc accaccgcag caaacagcgc tgacggtaaa tgaccatgat gaagtcaccg
      361 tctggaatgc cgcaccctgc cccatgctgc cccgcgacca ggtatacgtc cgcgtcgagg
      421 ccgtggcgat caatcccagt gacacgaaga tgcgcggaca gtttgccacg ccctgggcgt
      481 ttctcggaac ggactatgcc ggcacggtcg tcgcagtggg ttcggacgtg actcatatcc
      541 aagtgggtga ccgggtctac ggggcacaga acgagatgtg cccacgcacc ccggatcagg
      601 gggcattctc gcagtacacg gtcacgcgag gccgtgtttg ggccaagatc cccaagggct
      661 tgtcgttcga gcaggctgcc gcgctacctg cgggcatcag taccgctgga ttggcgatga
      721 agttgcttgg gctgcctttg ccatcgcctt cggcagacca gccacccacc cactccaagc
      781 cggtgtatgt gttggtctat gggggcagta cggccactgc cactgtcact atgcaaatgc
      841 tccgcctgtc cggatatatt ccaattgcaa catgctcccc ccacaatttc gacctggcca
      901 aatcgcgcgg cgcagaggag gtctttgact atcgggcccc gaatctcgcg cagacgatcc
      961 gtacctacac caagaacaat ctccgctatg ctctcgactg tatcaccaac gtcgagtcca
     1021 ccacattctg cttcgcagcc atcggccgcg cgggggggca ctacgtctcc ctgaacccgt
     1081 tccctgaaca cgcggccacg cgcaagatgg tcacgaccga ctggaccctg gggccgacca
     1141 tctttggcga gggatcaacc tggcccgccc cctatgggcg tcccggcagt gaggaagagc
     1201 ggcagttcgg cgaggatctg tggcgcatcg cggggcagct cgtcgaagat ggacgcctcg
     1261 tccatcatcc gttgcgcgtg gtgcagggcg gcttcgatca cattaagcaa ggcatggagc
     1321 tcgtccggaa gggagagctg tcgggggaga aactcgtggt tcggctcgag gggccgcatc
     1381 atcatcatca tcattgagtt tgtagcctta gacatgactg ttcctcagtt caagttgggc
     1441 acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa tgccatttgc
     1501 ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat agtataggat
     1561 tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct atctcgcagc
     1621 tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt ttttcttggt
     1681 atttcccact cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg tgcggatccc
     1741 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
     1801 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa ttttccctct
     1861 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac
     1921 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
     1981 cttgaaattt ttttttttag tttttttctc tttcagtgac ctccattgat atttaagtta
     2041 ataaacggtc ttcaatttct caagtttcag tttcattttt cttgttctat tacaactttt
     2101 tttacttctt gttcattaga aagaaagcat agcaatctaa tctaaggggc ggtgttgaca
     2161 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     2221 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     2281 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     2341 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     2401 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     2461 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     2521 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     2581 aggagcagga ctgacacgtc cgacggcggc ccacgggtcc caggcctcgg agatccgtcc
     2641 cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc
     2701 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     2761 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     2821 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     2881 ttgggacgct cgaaggcttt aatttgcaag ctggagacca acatgtgagc aaaaggccag
     2941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     3181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3421 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tga
//
