LOCUS Exported 11696 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3CA R93P. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CA-R93P SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11696) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11696 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6656..6680) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6688..6706 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6731..6750) /label=T7 /note="T7 promoter, forward primer" promoter complement(6732..6750) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6756..6776) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6830..6871 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6892..7459 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6909..7461 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7053..7070) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7280..7299 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7473..8072 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7473..7492) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7969..7989 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8086..8674 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8139..8159) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8557..8568) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8749..8982 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9101..9119) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9187..9291 /gene="bla" /label=AmpR promoter CDS 9292..10152 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9510..9529) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10323..10911 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10812..10831 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11065..11082 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11174..11193 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11322..11343 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11358..11388 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11396..11412 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11401..11423 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11420..11436 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11420..11436 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11486..11505 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg 3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata 3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa 3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta 3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac 3721 cttccgcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag 3781 atcctcaatc gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt 3841 aaagatccag aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg 3901 gatcttaggg acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta 3961 gaatcttcac cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata 4021 gtggtgatct gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc 4081 aaccatgact gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt 4141 atgttgctat cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt 4201 ttaaaagtgt gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag 4261 tatataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa 4321 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt 4381 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat 4441 agtgcactca gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt 4501 gataagatct atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg 4561 aacactcaaa gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata 4621 tacattcctg atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc 4681 cgaaagggtg ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat 4741 tacacagaca ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctcatgga 4801 ttagaagatt tgctgaaccc tattggtgtt actggatcaa atccaaataa agaaactcca 4861 tgcttagagt tggagtttga ctggttcagc agtgtggtaa agttcccaga tatgtcagtg 4921 attgaagagc atgccaattg gtctgtatcc cgagaagcag gatttagcta ttcccacgca 4981 ggactgagta acagactagc tagagacaat gaattaaggg aaaatgacaa agaacagctc 5041 aaagcaattt ctacacgaga tcctctctct gaaatcactg agcaggagaa agattttcta 5101 tggagtcaca gacactattg tgtaactatc cccgaaattc tacccaaatt gcttctgtct 5161 gttaaatgga attctagaga tgaagtagcc cagatgtatt gcttggtaaa agattggcct 5221 ccaatcaaac ctgaacaggc tatggaactt ctggactgta attacccaga tcctatggtt 5281 cgaggttttg ctgttcggtg cttggaaaaa tatttaacag atgacaaact ttctcagtat 5341 ttaattcagc tagtacaggt cctaaaatat gaacaatatt tggataactt gcttgtgaga 5401 tttttactga agaaagcatt gactaatcaa aggattgggc actttttctt ttggcattta 5461 aaatctgaga tgcacaataa aacagttagc cagaggtttg gcctgctttt ggagtcctat 5521 tgtcgtgcat gtgggatgta tttgaagcac ctgaataggc aagtcgaggc aatggaaaag 5581 ctcattaact taactgacat tctcaaacag gagaagaagg atgaaacaca aaaggtacag 5641 atgaagtttt tagttgagca aatgaggcga ccagatttca tggatgctct acagggcttt 5701 ctgtctcctc taaaccctgc tcatcaacta ggaaacctca ggcttgaaga gtgtcgaatt 5761 atgtcctctg caaaaaggcc actgtggttg aattgggaga acccagacat catgtcagag 5821 ttactgtttc agaacaatga gatcatcttt aaaaatgggg atgatttacg gcaagatatg 5881 ctaacacttc aaattattcg tattatggaa aatatctggc aaaatcaagg tcttgatctt 5941 cgaatgttac cttatggttg tctgtcaatc ggtgactgtg tgggacttat tgaggtggtg 6001 cgaaattctc acactattat gcaaattcag tgcaaaggcg gcttgaaagg tgcactgcag 6061 ttcaacagcc acacactaca tcagtggctc aaagacaaga acaaaggaga aatatatgat 6121 gcagccattg acctgtttac acgttcatgt gctggatact gtgtagctac cttcattttg 6181 ggaattggag atcgtcacaa tagtaacatc atggtgaaag acgatggaca actgtttcat 6241 atagattttg gacacttttt ggatcacaag aagaaaaaat ttggttataa acgagaacgt 6301 gtgccatttg ttttgacaca ggatttctta atagtgatta gtaaaggagc ccaagaatgc 6361 acaaagacaa gagaatttga gaggtttcag gagatgtgtt acaaggctta tctagctatt 6421 cgacagcatg ccaatctctt cataaatctt ttctcaatga tgcttggctc tggaatgcca 6481 gaactacaat cttttgatga cattgcatac attcgaaaga ccctagcctt agataaaact 6541 gagcaagagg ctttggagta tttcatgaaa caaatgaatg atgcacatca tggtggctgg 6601 acaacaaaaa tggattggat cttccacaca attaaacagc atgcattgaa ctagaaccca 6661 gctttcttgt acaaagtggt gatatccaat taaccctcac taaaggtagt agaattgttg 6721 ttaaagaatg ccctatagtg agtcgtatta ccacccaact tttctataca aagtggttga 6781 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat 6841 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc 6901 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat 6961 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt 7021 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt 7081 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc 7141 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc 7201 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat 7261 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc 7321 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg 7381 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct 7441 ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc 7501 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 7561 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 7621 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 7681 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 7741 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 7801 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 7861 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 7921 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 7981 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 8041 cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat 8101 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 8161 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 8221 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 8281 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 8341 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 8401 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 8461 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 8521 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 8581 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 8641 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt 8701 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 8761 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 8821 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 8881 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 8941 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 9001 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt 9061 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt 9121 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 9181 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 9241 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 9301 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 9361 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 9421 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 9481 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac 9541 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 9601 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 9661 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 9721 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 9781 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 9841 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 9901 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 9961 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 10021 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 10081 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 10141 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 10201 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 10261 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 10321 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 10381 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 10441 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 10501 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 10561 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 10621 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 10681 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 10741 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 10801 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 10861 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 10921 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 10981 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 11041 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 11101 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct 11161 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 11221 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac 11281 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 11341 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 11401 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa 11461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 11521 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac 11581 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 11641 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //