LOCUS Exported 11621 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3CD R927H. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CD-R927H SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11621) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11621) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11621 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6581..6605) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6613..6631 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6656..6675) /label=T7 /note="T7 promoter, forward primer" promoter complement(6657..6675) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6681..6701) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6755..6796 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6817..7384 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6834..7386 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6978..6995) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7205..7224 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7398..7997 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7398..7417) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7894..7914 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8011..8599 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8064..8084) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8482..8493) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8674..8907 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9026..9044) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9112..9216 /gene="bla" /label=AmpR promoter CDS 9217..10077 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9435..9454) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10248..10836 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10737..10756 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10990..11007 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11099..11118 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11247..11268 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11283..11313 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11321..11337 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11326..11348 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11345..11361 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11345..11361 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11411..11430 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcaccatg ccccctgggg tggactgccc catggaattc 3481 tggaccaagg aggagaatca gagcgttgtg gttgacttcc tgctgcccac aggggtctac 3541 ctgaacttcc ctgtgtcccg caatgccaac ctcagcacca tcaagcagct gctgtggcac 3601 cgcgcccagt atgagccgct cttccacatg ctcagtggcc ccgaggccta tgtgttcacc 3661 tgcatcaacc agacagcgga gcagcaagag ctggaggacg agcaacggcg tctgtgtgac 3721 gtgcagccct tcctgcccgt cctgcgcctg gtggcccgtg agggcgaccg cgtgaagaag 3781 ctcatcaact cacagatcag cctcctcatc ggcaaaggcc tccacgagtt tgactccttg 3841 tgcgacccag aagtgaacga ctttcgcgcc aagatgtgcc aattctgcga ggaggcggcc 3901 gcccgccggc agcagctggg ctgggaggcc tggctgcagt acagtttccc cctgcagctg 3961 gagccctcgg ctcaaacctg ggggcctggt accctgcggc tcccgaaccg ggcccttctg 4021 gtcaacgtta agtttgaggg cagcgaggag agcttcacct tccaggtgtc caccaaggac 4081 gtgccgctgg cgctgatggc ctgtgccctg cggaagaagg ccacagtgtt ccggcagccg 4141 ctggtggagc agccggaaga ctacacgctg caggtgaacg gcaggcatga gtacctgtat 4201 ggcagctacc cgctctgcca gttccagtac atctgcagct gcctgcacag tgggttgacc 4261 cctcacctga ccatggtcca ttcctcctcc atcctcgcca tgcgggatga gcagagcaac 4321 cctgcccccc aggtccagaa accgcgtgcc aaaccacctc ccattcctgc gaagaagcct 4381 tcctctgtgt ccctgtggtc cctggagcag ccgttccgca tcgagctcat ccagggcagc 4441 aaagtgaacg ccgacgagcg gatgaagctg gtggtgcagg ccgggctttt ccacggcaac 4501 gagatgctgt gcaagacggt gtccagctcg gaggtgagcg tgtgctcgga gcccgtgtgg 4561 aagcagcggc tggagttcga catcaacatc tgcgacctgc cccgcatggc ccgtctctgc 4621 tttgcgctgt acgccgtgat cgagaaagcc aagaaggctc gctccaccaa gaagaagtcc 4681 aagaaggcgg actgccccat tgcctgggcc aacctcatgc tgtttgacta caaggaccag 4741 cttaagaccg gggaacgctg cctctacatg tggccctccg tcccagatga gaagggcgag 4801 ctgctgaacc ccacgggcac tgtgcgcagt aaccccaaca cggatagcgc cgctgccctg 4861 ctcatctgcc tgcccgaggt ggccccgcac cccgtgtact accccgccct ggagaagatc 4921 ttggagctgg ggcgacacag cgagtgtgtg catgtcaccg aggaggagca gctgcagctg 4981 cgggaaatcc tggagcggcg ggggtctggg gagctgtatg agcacgagaa ggacctggtg 5041 tggaagctgc ggcatgaagt ccaggagcac ttcccggagg cgctagcccg gctgctgctg 5101 gtcaccaagt ggaacaagca tgaggatgtg gcccagatgc tctacctgct gtgctcctgg 5161 ccggagctgc ccgtcctgag cgccctggag ctgctagact tcagcttccc cgattgccac 5221 gtaggctcct tcgccatcaa gtcgctgcgg aaactgacgg acgatgagct gttccagtac 5281 ctgctgcagc tggtgcaggt gctcaagtac gagtcctacc tggactgcga gctgaccaaa 5341 ttcctgctgg accgggccct ggccaaccgc aagatcggcc acttcctttt ctggcacctc 5401 cgctccgaga tgcacgtgcc gtcggtggcc ctgcgcttcg gcctcatcct ggaggcctac 5461 tgcaggggca gcacccacca catgaaggtg ctgatgaagc agggggaagc actgagcaaa 5521 ctgaaggccc tgaatgactt cgtcaagctg agctctcaga agacccccaa gccccagacc 5581 aaggagctga tgcacttgtg catgcggcag gaggcctacc tagaggccct ctcccacctg 5641 cagtccccac tcgaccccag caccctgctg gctgaagtct gcgtggagca gtgcaccttc 5701 atggactcca agatgaagcc cctgtggatc atgtacagca acgaggaggc aggcagcggc 5761 ggcagcgtgg gcatcatctt taagaacggg gatgacctcc ggcaggacat gctgaccctg 5821 cagatgatcc agctcatgga cgtcctgtgg aagcaggagg ggctggacct gaggatgacc 5881 ccctatggct gcctccccac cggggaccgc acaggcctca ttgaggtggt actccgttca 5941 gacaccatcg ccaacatcca actcaacaag agcaacatgg cagccacagc cgccttcaac 6001 aaggatgccc tgctcaactg gctgaagtcc aagaacccgg gggaggccct ggatcgagcc 6061 attgaggagt tcaccctctc ctgtgctggc tattgtgtgg ccacatatgt gctgggcatt 6121 ggcgatcggc acagcgacaa catcatgatc cgagagagtg ggcagctgtt ccacattgat 6181 tttggccact ttctggggaa tttcaagacc aagtttggaa tcaaccacga gcgtgtccca 6241 ttcatcctca cctacgactt tgtccatgtg attcagcagg ggaagactaa taatagtgag 6301 aaatttgaac ggttccgggg ctactgtgaa agggcctaca ccatcctgcg gcgccacggg 6361 cttctcttcc tccacctctt tgccctgatg cgggcggcag gcctgcctga gctcagctgc 6421 tccaaagaca tccagtatct caaggactcc ctggcactgg ggaaaacaga ggaggaggca 6481 ctgaagcact tccgagtgaa gtttaacgaa gccctccgtg agagctggaa aaccaaagtg 6541 aactggctgg cccacaacgt gtccaaagac aacaggcagt tgccaacttt cttgtacaaa 6601 gtggtgatat ccaattaacc ctcactaaag gtataagaag taatttgaga agagtcccta 6661 tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc agcacagtgg 6721 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct 6781 cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc ntaacgttac 6841 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat 6901 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat 6961 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga 7021 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca 7081 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac 7141 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt 7201 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca 7261 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt 7321 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg 7381 ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 7441 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 7501 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 7561 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 7621 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 7681 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 7741 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 7801 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 7861 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 7921 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 7981 gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa 8041 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 8101 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 8161 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 8221 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 8281 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 8341 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 8401 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 8461 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 8521 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 8581 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag 8641 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 8701 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 8761 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 8821 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 8881 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 8941 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg 9001 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 9061 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 9121 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 9181 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 9241 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 9301 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 9361 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 9421 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa 9481 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 9541 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 9601 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 9661 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 9721 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 9781 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 9841 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 9901 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 9961 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 10021 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 10081 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 10141 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 10201 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 10261 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 10321 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 10381 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 10441 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 10501 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 10561 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 10621 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 10681 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 10741 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 10801 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 10861 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 10921 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 10981 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 11041 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta 11101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 11161 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg 11221 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 11281 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 11341 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg 11401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 11461 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 11521 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 11581 agaaattgta tttgttaaat atgtactaca aacttagtag t //