LOCUS Exported 11798 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3CG D192G. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CG-D192G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11798) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11798) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11798 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6354..6365 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" protein_bind complement(6758..6782) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6790..6808 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6833..6852) /label=T7 /note="T7 promoter, forward primer" promoter complement(6834..6852) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6858..6878) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6932..6973 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6994..7561 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7011..7563 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7155..7172) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7382..7401 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7575..8174 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7575..7594) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8071..8091 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8188..8776 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8241..8261) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8659..8670) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8851..9084 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9203..9221) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9289..9393 /gene="bla" /label=AmpR promoter CDS 9394..10254 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9612..9631) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10425..11013 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10914..10933 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11167..11184 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11276..11295 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11424..11445 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11460..11490 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11498..11514 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11503..11525 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11522..11538 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11522..11538 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11588..11607 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gagctggaga actataaaca gcccgtggtg 3481 ctgagagagg acaactgccg aaggcgccgg aggatgaagc cgcgcagtgc tgcggccagc 3541 ctgtcctcca tggagctcat ccccatcgag ttcgtgctgc ccaccagcca gcgcaaatgc 3601 aagagccccg aaacggcgct gctgcacgtg gccggccacg gcaacgtgga gcagatgaag 3661 gcccaggtgt ggctgcgagc gctggagacc agcgtggcgg cggacttcta ccaccggctg 3721 ggaccgcatc acttcctcct gctctatcag aagaaggggc agtggtacga gatctacgac 3781 aagtaccagg tggtgcagac tctggactgc ctgcgctact ggaaggccac gcaccggagc 3841 ccgggccaga tccacctggt gcagcggcac ccgccctccg aggagtccca agccttccag 3901 cggcagctca cggcgctgat tggctatgac gtcactgacg tcagcaacgt gcacgacgat 3961 gagctggagt tcacgcgccg tggcttggtg accccgcgca tggcggaggt ggccagccgc 4021 ggccccaagc tctacgccat gcacccgtgg gtgacgtcca agcccctccc ggagtacctg 4081 tggaagaaga ttgccaacaa ctgcatcttc atcgtcattc accgcagcac caccagccag 4141 accattaagg tctcacccga cgacaccccc ggcgccatcc tgcagagctt cttcaccaag 4201 atggccaaga agaaatctct gatggatatt cccgaaagcc aaagcgaaca ggattttgtg 4261 ctgcgcgtct gtggccggga tgagtacctg gtgggcgaaa cgcccatcaa aaacttccag 4321 tgggtgaggc actgcctcaa gaacggagaa gagattcacg tggtactgga cacgcctcca 4381 gacccggccc tagacgaggt gaggaaggaa gagtggccgc tggtggatga ctgcacggga 4441 gtcaccggct accatgagca gcttaccatc cacggcaagg accacgagag tgtgttcacc 4501 gtgtccctgt gggactgcga ccgcaagttc agggtcaaga tcagaggcat tgatatcccc 4561 gtcctgcctc ggaacaccga cctcacagtt tttgtagagg caaacatcca gcatgggcaa 4621 caagtccttt gccaaaggag aaccagcccc aaacccttca cagaggaggt gctgtggaat 4681 gtgtggcttg agttcagtat caaaatcaaa gacttgccca aaggggctct actgaacctc 4741 cagatctact gcggtaaagc tccagcactg tccagcaagg cctctgcaga gtcccccagt 4801 tctgagtcca agggcaaagt tcagcttctc tattatgtga acctgctgct gatagaccac 4861 cgtttcctcc tgcgccgtgg agaatacgtc ctccacatgt ggcagatatc tgggaaggga 4921 gaagaccaag gaagcttcaa tgctgacaaa ctcacgtctg caactaaccc agacaaggag 4981 aactcaatgt ccatctccat tcttctggac aattactgcc acccgatagc cctgcctaag 5041 catcagccca cccctgaccc ggaaggggac cgggttcgag cagaaatgcc caaccagctt 5101 cgcaagcaat tggaggcgat catagccact gatccactta accctctcac agcagaggac 5161 aaagaattgc tctggcattt tagatacgaa agccttaagc acccaaaagc atatcctaag 5221 ctatttagtt cagtgaaatg gggacagcaa gaaattgtgg ccaaaacata ccaattgttg 5281 gccagaaggg aagtctggga tcaaagtgct ttggatgttg ggttaacaat gcagctcctg 5341 gactgcaact tctcagatga aaatgtaaga gccattgcag ttcagaaact ggagagcttg 5401 gaggacgatg atgttctgca ttaccttcta caattggtcc aggctgtgaa atttgaacca 5461 taccatgata gcgcccttgc cagatttctg ctgaagcgtg gtttaagaaa caaaagaatt 5521 ggtcactttt tgttttggtt cttgagaagt gagatagccc agtccagaca ctatcagcag 5581 aggttcgctg tgattctgga agcctatctg aggggctgtg gcacagccat gctgcacgac 5641 tttacccaac aagtccaagt aatcgagatg ttacaaaaag tcacccttga tattaaatcg 5701 ctctctgctg aaaagtatga cgtcagttcc caagttattt cacaacttaa acaaaagctt 5761 gaaaacctgc agaattctca actccccgaa agctttagag ttccatatga tcctggactg 5821 aaagcaggag cgctggcaat tgaaaaatgt aaagtaatgg cctccaagaa aaaaccacta 5881 tggcttgagt ttaaatgtgc cgatcctaca gccctatcaa atgaaacaat tggaattatc 5941 tttaaacatg gtgatgatct gcgccaagac atgcttattt tacagattct acgaatcatg 6001 gagtctattt gggagactga atctttggat ctatgcctcc tgccatatgg ttgcatttca 6061 actggtgaca aaataggaat gatcgagatt gtgaaagacg ccacgacaat tgccaaaatt 6121 cagcaaagca cagtgggcaa cacgggagca tttaaagatg aagtcctgaa tcactggctc 6181 aaagaaaaat cccctactga agaaaagttt caggcagcag tggagagatt tgtttattcc 6241 tgtgcaggct actgtgtggc aacctttgtt cttggaatag gcgacagaca caatgacaat 6301 attatgatca ccgagacagg aaacctattt catattgact tcgggcacat tcttgggaat 6361 tacaaaagtt tcctgggcat taataaagag agagtgccat ttgtgctaac ccctgacttc 6421 ctctttgtga tgggaacttc tggaaagaag acaagcccac acttccagaa atttcaggac 6481 atctgtgtta aggcttatct agcccttcgt catcacacaa acctactgat catcctgttc 6541 tccatgatgc tgatgacagg aatgccccag ttaacaagca aagaagacat tgaatatatc 6601 cgggatgccc tcacagtggg gaaaaatgag gaggatgcta aaaagtattt tcttgatcag 6661 atcgaagttt gcagagacaa aggatggact gtgcagttta attggtttct acatcttgtt 6721 cttggcatca aacaaggaga gaaacattca gcctagaacc cagctttctt gtacaaagtg 6781 gtgatatcca attaaccctc actaaaggtt gtgatagtag ttagatattt gtccctatag 6841 tgagtcgtat taccacccaa cttttctata caaagtggtt gatatccagc acagtggcgg 6901 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg 6961 tctcgattct acgcgtaccg gttagtaatg agatccctcc ccccccccta acgttactgg 7021 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 7081 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 7141 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 7201 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 7261 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 7321 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 7381 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 7441 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 7501 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 7561 atatggccac acatatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 7621 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 7681 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 7741 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 7801 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 7861 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 7921 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 7981 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 8041 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 8101 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 8161 agcccggtgc ctaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga 8221 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 8281 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 8341 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 8401 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 8461 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 8521 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 8581 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 8641 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 8701 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 8761 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc 8821 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 8881 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 8941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 9001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 9061 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 9121 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta 9181 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 9241 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 9301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 9361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 9421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 9481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 9541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 9601 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 9661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 9721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 9781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 9841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 9901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 9961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 10021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 10081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 10141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 10201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 10261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 10321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 10381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 10441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 10501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 10561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 10621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 10681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 10741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 10801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 10861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 10921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 10981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 11041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 11101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 11161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 11221 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt 11281 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 11341 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa 11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 11521 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat 11581 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata 11641 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat 11701 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 11761 aattgtattt gttaaatatg tactacaaac ttagtagt //