LOCUS Exported 9989 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Entry plasmid for guide cloning. ACCESSION . VERSION . KEYWORDS CROPseq-Guide-Zeo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9989) AUTHORS Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity AJ, Zhang F, Blainey PC TITLE Optical Pooled Screens in Human Cells. JOURNAL Cell. 2019 Oct 17;179(3):787-799.e17. doi: 10.1016/j.cell.2019.09.016. PUBMED 31626775 REFERENCE 2 (bases 1 to 9989) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9989 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 50..290 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 50..70 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 221..240 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 2180..2255 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" LTR 2308..2488 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 2566..2687 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2603..2622) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2657..2676 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 2727..2862 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2789..2808 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(2882..2901) /label=T7 /note="T7 promoter, forward primer" promoter complement(2883..2901) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2911..2928) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2911..2927) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2920..2942) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3069..3524 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3156..3175) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3366..3387 /label=F1ori-F /note="F1 origin, forward primer" promoter 3550..3654 /gene="bla" /label=AmpR promoter CDS 3655..4515 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3873..3892) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4686..5274 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5175..5194 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5428..5445 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5562..5583 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5598..5628 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5636..5652 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5641..5663 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5660..5676 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5660..5676 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 5695..5715 /label=T3 /note="T3 promoter, forward primer" promoter 5697..5715 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 5743..5969 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 5970..6150 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 6197..6322 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6815..7048 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7233..7277 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 7458..7575 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7714..8892 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 7945..8883 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 8840..8860 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 8899..8908 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8905..9279 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" misc_feature 9295..9883 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9348..9368) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9766..9777) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt 61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat 121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt 181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt 241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg 301 agacggttgt aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt 361 ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag aatttttatg 421 ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac 481 tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag 541 ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa 601 ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg 661 attttgaaaa tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac 721 aagctttcat gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat 781 gtgatccagc tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt 841 cttttaatgc accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt 901 cagaaacttc gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat 961 ttgttattcc tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat 1021 taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag 1081 ctttcgtagc atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt 1141 ctcctatata ttcattttta attttaattc tttaataatt tcgtctactt taactttagc 1201 gttttgaaca gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact 1261 tgggcgaaca gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg 1321 catattatac attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg 1381 agttaagggt gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc 1441 tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc 1501 ttctaaatag tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat 1561 taatatagaa gcttgtattc catctttatc tctagctgag tcatcaatta catatccata 1621 actttcttca taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct 1681 acccattcat ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc 1741 gattctatca cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat 1801 gctatttaag cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt 1861 tccatctaat cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc 1921 tgggtcattc ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt 1981 tcatgcagga tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa 2041 tgcatgctct tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa 2101 tttagttcct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg 2161 ctcttttttg tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc 2221 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat 2281 cttgagacac tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 2341 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 2401 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 2461 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt 2521 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 2581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 2641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 2701 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 2761 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 2821 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 2881 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 2941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 3001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 3061 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 3121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 3181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 3241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 3301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 3361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 3421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 3481 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 3541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 3601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 3661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 3721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 3841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 3901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 3961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 4021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 4081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 4141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 4201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 4261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 4321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 4381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 4441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 4501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 4561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4801 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 4861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 4921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 4981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 5341 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 5401 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 5461 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 5521 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 5581 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 5641 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 5701 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac 5761 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa 5821 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc 5881 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg 5941 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct 6001 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 6061 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 6121 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 6181 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 6241 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 6301 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 6361 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 6421 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 6481 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 6541 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 6601 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 6661 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 6721 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 6781 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 6841 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 6901 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 6961 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 7021 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 7081 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 7141 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 7201 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 7261 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 7321 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 7381 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt gagacaaatg 7441 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 7501 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 7561 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc 7621 gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa 7681 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag 7741 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc 7801 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 7861 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 7921 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 7981 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt 8041 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 8101 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 8161 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 8221 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 8281 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 8341 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 8401 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 8461 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 8521 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 8581 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 8641 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 8701 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 8761 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 8821 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 8881 caggtgtcgt gacgtacggc caccatggcc aagttgacca gtgccgttcc ggtgctcacc 8941 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac 9001 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg 9061 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac 9121 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg 9181 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc 9241 ggcaactgcg tgcacttcgt ggccgaggag caggactgaa cgcgttaagt cgacaatcaa 9301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 9361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 9421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 9481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 9541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 9601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 9661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 9721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 9781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 9841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa 9901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 9961 ggctaattca ctcccaacga agacaagat //