LOCUS       Exported               10323 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABEpuroUThTERT+U3-hTR-500
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Wong JM, Collins K
  TITLE     Telomerase RNA level limits telomere maintenance in X-linked 
            dyskeratosis congenita.
  JOURNAL   Genes Dev. 2006 Oct 15. 20(20):2848-58.
  PUBMED    17015423
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1431..4829
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(4841..4861)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4846..5175
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5026..5161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5088..5107
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5185..5784
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5185..5204)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5681..5701
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7257..7273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7257..7273)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7305..7324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7306..7324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7331..7348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7331..7347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7340..7362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7576..7595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     LTR             7810..8279
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8409..8426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8580..9168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8660..8679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9328..10188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9951..9970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10189..10293)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcgtcct gctgcgcacg tgggaagccc tggccccggc cacccccgcg atgccgcgcg
     1441 ctccccgctg ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag gtgctgccgc
     1501 tggccacgtt cgtgcggcgc ctggggcccc agggctggcg gctggtgcag cgcggggacc
     1561 cggcggcttt ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg gacgcacggc
     1621 cgccccccgc cgccccctcc ttccgccagg tgtcctgcct gaaggagctg gtggcccgag
     1681 tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc
     1741 tggacggggc ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc agctacctgc
     1801 ccaacacggt gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ctgcgccgcg
     1861 tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc
     1921 ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg
     1981 cccggccccc gccacacgct agtggacccc gaaggcgtct gggatgcgaa cgggcctgga
     2041 accatagcgt cagggaggcc ggggtccccc tgggcctgcc agccccgggt gcgaggaggc
     2101 gcgggggcag tgccagccga agtctgccgt tgcccaagag gcccaggcgt ggcgctgccc
     2161 ctgagccgga gcggacgccc gttgggcagg ggtcctgggc ccacccgggc aggacgcgtg
     2221 gaccgagtga ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa gaagccacct
     2281 ctttggaggg tgcgctctct ggcacgcgcc actcccaccc atccgtgggc cgccagcacc
     2341 acgcgggccc cccatccaca tcgcggccac cacgtccctg ggacacgcct tgtcccccgg
     2401 tgtacgccga gaccaagcac ttcctctact cctcaggcga caaggagcag ctgcggccct
     2461 ccttcctact cagctctctg aggcccagcc tgactggcgc tcggaggctc gtggagacca
     2521 tctttctggg ttccaggccc tggatgccag ggactccccg caggttgccc cgcctgcccc
     2581 agcgctactg gcaaatgcgg cccctgtttc tggagctgct tgggaaccac gcgcagtgcc
     2641 cctacggggt gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc ccagcagccg
     2701 gtgtctgtgc ccgggagaag ccccagggct ctgtggcggc ccccgaggag gaggacacag
     2761 acccccgtcg cctggtgcag ctgctccgcc agcacagcag cccctggcag gtgtacggct
     2821 tcgtgcgggc ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc aggcacaacg
     2881 aacgccgctt cctcaggaac accaagaagt tcatctccct ggggaagcat gccaagctct
     2941 cgctgcagga gctgacgtgg aagatgagcg tgcggggctg cgcttggctg cgcaggagcc
     3001 caggggttgg ctgtgttccg gccgcagagc accgtctgcg tgaggagatc ctggccaagt
     3061 tcctgcactg gctgatgagt gtgtacgtcg tcgagctgct caggtctttc ttttatgtca
     3121 cggagaccac gtttcaaaag aacaggctct ttttctaccg gaagagtgtc tggagcaagt
     3181 tgcaaagcat tggaatcaga cagcacttga agagggtgca gctgcgggag ctgtcggaag
     3241 cagaggtcag gcagcatcgg gaagccaggc ccgccctgct gacgtccaga ctccgcttca
     3301 tccccaagcc tgacgggctg cggccgattg tgaacatgga ctacgtcgtg ggagccagaa
     3361 cgttccgcag agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca ctgttcagcg
     3421 tgctcaacta cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg ctgggcctgg
     3481 acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag gacccgccgc
     3541 ctgagctgta ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc ccccaggaca
     3601 ggctcacgga ggtcatcgcc agcatcatca aaccccagaa cacgtactgc gtgcgtcggt
     3661 atgccgtggt ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag agccacgtct
     3721 ctaccttgac agacctccag ccgtacatgc gacagttcgt ggctcacctg caggagacca
     3781 gcccgctgag ggatgccgtc gtcatcgagc agagctcctc cctgaatgag gccagcagtg
     3841 gcctcttcga cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc aggggcaagt
     3901 cctacgtcca gtgccagggg atcccgcagg gctccatcct ctccacgctg ctctgcagcc
     3961 tgtgctacgg cgacatggag aacaagctgt ttgcggggat tcggcgggac gggctgctcc
     4021 tgcgtttggt ggatgatttc ttgttggtga cacctcacct cacccacgcg aaaaccttcc
     4081 tcaggaccct ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg cggaagacag
     4141 tggtgaactt ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt cagatgccgg
     4201 cccacggcct attcccctgg tgcggcctgc tgctggatac ccggaccctg gaggtgcaga
     4261 gcgactactc cagctatgcc cggacctcca tcagagccag tctcaccttc aaccgcggct
     4321 tcaaggctgg gaggaacatg cgtcgcaaac tctttggggt cttgcggctg aagtgtcaca
     4381 gcctgtttct ggatttgcag gtgaacagcc tccagacggt gtgcaccaac atctacaaga
     4441 tcctcctgct gcaggcgtac aggtttcacg catgtgtgct gcagctccca tttcatcagc
     4501 aagtttggaa gaaccccaca tttttcctgc gcgtcatctc tgacacggcc tccctctgct
     4561 actccatcct gaaagccaag aacgcaggga tgtcgctggg ggccaagggc gccgccggcc
     4621 ctctgccctc cgaggccgtg cagtggctgt gccaccaagc attcctgctc aagctgactc
     4681 gacaccgtgt cacctacgtg ccactcctgg ggtcactcag gacagcccag acgcagctga
     4741 gtcggaagct cccggggacg acgctgactg ccctggaggc cgcagccaac ccggcactgc
     4801 cctcagactt caagaccatc ctggactgag tcgaccctgt ggaatgtgtg tcagttaggg
     4861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4921 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4981 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5101 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5161 ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     5221 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     5281 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     5341 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     5401 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     5461 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5521 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5581 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5641 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5701 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5761 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     5821 agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     5881 ggaatgaaag accccacctg taggtttggc aagctagacg gatcgggaga tcttcgcaaa
     5941 acgctgggat tcccggatta caggcgggcg caccacacca ggagcaaaca cttccggttt
     6001 taaaaattca gtttgtgatt ggctgtcatt cagtattatg ctaattaagc atgcccggtt
     6061 ttaaacctct taaaacaact tttaaaatta cctttccacc taaaacgtta aaatttgtca
     6121 agtgataata ttcgaaaagc tgttattgcc aaactatttt cctatttgtt tcctaatggc
     6181 atcggaacta gcgaaagttt ctcgccatca gttaaaagtt tgcggcagat gtagacctag
     6241 cagaggtgtg cgaggagaag cttgggttgc ggagggtggg cctgggaggg gtggtggcca
     6301 ttttttgtct aaccctaact gagaagggcg taggcgccgt gcttttgctc cccgcgcgct
     6361 gtttttctcg ctgactttca gcgggcggaa aagcctcggc ctgccgcctt ccaccgttca
     6421 ttctagagca aacaaaaaat gtcagctgct ggcccgttcg cccctcccgg ggacctgcgg
     6481 cgggtcgcct gcccagcccc cgaaccccgc ctggaggccg cggtcggccc ggggcttctc
     6541 cggaggcacc cactgccacc gcgaagagtt gggctctgtc agccgcgggt ctctcggggg
     6601 cgagggcgag gttcaggcct ttcaggccgc aggaagagga acggagcgag tccccgcgcg
     6661 cggcgcgatt ccctgagctg tgggacgtgc acccaggact cggctcacac atgcagttcg
     6721 ctttcctgtt ggtgggggga acgccgatcg tgcgcatccg tcacccctcg ccggcagtgg
     6781 gggcttgtga acccccaaac ctgactgact gggccagtgt gctgcaaatt ggcaggagac
     6841 gtgaaggcac ctccaaagtc ggccaaaatg aatgggcagt gagccggggt tgcctggagc
     6901 cgttcctgcg tgggttctcc cgtcttccgc tttttgttgc cttttatggt tgtattacaa
     6961 cttagttcct gctctgcaga ttttgttgag gtttttgctt ctcccaaggt agatctcgac
     7021 cagtcccctc aacggggtgt gggagaacag tcattttttt ttgagagatc atttaacatt
     7081 taatgaatat ttaattagaa gatctaaatg aacattggaa attgtgttcc tttaatggtc
     7141 atcggtttat gccagaggtt agaagtttct tttttgaaaa attagacctt ggcgatgacc
     7201 ttgagcagta ggatataacc cccacaagct tgatatcgaa ttcctgcagc ccgggggatc
     7261 cactagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt
     7321 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7381 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7441 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     7501 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7561 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7621 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7681 acctcgaccc caaaaaactt gattagggtg atggttcact agcttaagta acgccatttt
     7741 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     7801 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     7861 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     7921 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     7981 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     8041 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     8101 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     8161 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     8221 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     8281 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     8341 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9241 atgagattat caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag
     9301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
    10321 agc
//
