LOCUS Exported 4623 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Plasmid for making zebrafish apoA-IVa in situ probe. ACCESSION . VERSION . KEYWORDS PCRII apoA-IVa SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4623) AUTHORS Otis JP, Zeituni EM, Thierer JH, Anderson JL, Brown AC, Boehm ED, Cerchione DM, Ceasrine AM, Avraham-Davidi I, Tempelhof H, Yaniv K, Farber SA TITLE Zebrafish as a model for apolipoprotein biology: comprehensive expression analysis and a role for ApoA-IV in regulating food intake. JOURNAL Dis Model Mech. 2015 Mar;8(3):295-309. doi: 10.1242/dmm.018754. Epub 2015 Jan 29. PUBMED 25633982 REFERENCE 2 (bases 1 to 4623) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4623 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 1..17 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 35..53 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 35..52 /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(852..871) /label=T7 /note="T7 promoter, forward primer" promoter complement(853..871) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(878..895) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(878..894) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(887..909) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 1035..1463 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1122..1141) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1332..1353 /label=F1ori-F /note="F1 origin, forward primer" CDS 1807..2601 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(1861..1880) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2471..2490 /label=Neo-F /note="Neomycin resistance gene, forward primer" CDS 2619..3479 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2837..2856) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3650..4238 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4139..4158 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4392..4409 /label=L4440 /note="L4440 vector, forward primer" protein_bind 4526..4547 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4562..4592 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4600..4616 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4605..4 /label=M13/pUC Reverse /note="In lacZ gene" ORIGIN 1 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca 61 agctatgcat caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg 121 gaattcggct tgaacgagtc acacggcatg tagaaatgac agcagggcaa actttctgga 181 ctttactgcc tttatcagag ttttgtcgtc catttatata caacgcagat accctcgcac 241 ttacacacag aagaggacaa gagaagaacc agcaaataac catgaaagtc cttgtagtat 301 tcgtcatcgc agctttctct agctgtcatg ccaacatgat gtggcaggac caaccaatgc 361 ccagcatgga cctggtgaaa aatgctttct ggaattacgt ttctcaagcg accctcacag 421 ctgaggaaac acttcagatg atcagaaact cagagctggg tcaagaaatc aatgacagga 481 tctctaaaag cacagatgcc atcaataaat acaccctggc cattcaaagt caggtcactc 541 ctttgaccca agaactgctc accaaactgt cccaggaggc cgatcagctg aaactgagtc 601 tggagcaaga aatcacctcg gcacagaccc agctcaagcc ttatgtcgaa gaactcaaag 661 tgggcattga ggagcagatt gagcggctga aaaaggacgt ggctccatac gcagaggccc 721 tgaaccctga agccatgagg ttgaccctgg tcctgcgggc tgaagagctg aaggcaaagc 781 tggaagccga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag 841 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc 901 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 961 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 1021 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 1081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 1141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 1201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 1261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 1321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 1381 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 1441 ttaacaaaaa tttaacgcga attttaacaa aattcagggc gcaagggctg ctaaaggaag 1501 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac 1561 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg 1621 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca 1681 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg 1741 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg 1801 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 1861 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 1921 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 1981 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 2041 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 2101 gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat catggctgat 2161 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 2221 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 2281 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg 2341 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 2401 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 2461 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 2521 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 2581 cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa catttccgtg 2641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 2701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 2761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 2821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 2881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 2941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 3001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 3061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 3121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 3181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 3241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 3301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 3361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 3421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 3481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 3541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 3601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 3661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 3721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 3781 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 3841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 3901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 3961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 4021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 4081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 4141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 4201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 4261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 4321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 4381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 4441 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 4501 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 4561 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 4621 aca //