LOCUS       Exported               17531 bp ds-DNA     circular SYN 14-FEB-2022
DEFINITION  to overexpress human MALAT1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-hMALAT1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17531)
  AUTHORS   Hu Y, Lin J, Fang H, Fang J, Li C, Chen W, Liu S, Ondrejka S, Gong 
            Z, Reu F, Maciejewski J, Yi Q, Zhao JJ
  TITLE     Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and 
            apoptosis in multiple myeloma.
  JOURNAL   Leukemia. 2018 Mar 22. pii: 10.1038/s41375-018-0104-2. doi: 
            10.1038/s41375-018-0104-2.
  PUBMED    29632340
REFERENCE   2  (bases 1 to 17531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17531
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2373..2584
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2597..2865
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        11673..11884
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             11897..12165
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      12194..12203
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             12224..12886
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             12956..13009
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             13010..13609
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13010..13029)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13506..13526
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13610..14198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13663..13683)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14081..14092)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14272..14505
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    14577..14698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14614..14633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14668..14687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      14738..14873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14800..14819
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(14906..14922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14906..14922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14919..14941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14930..14946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14954..14984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14999..15020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15137..15154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15308..15896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15388..15407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16067..16927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16690..16709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16928..17032)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     17100..17118
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17156..17178)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     17278..17297
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17491..17513
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17505..17522
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17506..17522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttgaaa gaccccacct gtaggtttgg caagttagct taagtaacgc
     1981 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2041 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2101 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagcg
     2161 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2221 ctaaccaatc agtttgcttc ttgcttctgt ttgtgtgctt ctgctccctg agctcaataa
     2281 aagagcccac aacccctcac ttggtgggcc agtcctctga tagactgtgt cccctggata
     2341 cccgtattct aggcggccgc gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2401 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     2461 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2521 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2581 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     2641 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     2701 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     2761 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     2821 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatcgcggc cgcgtaaagg
     2881 actggggccc cgcaactggc ctctcctgcc ctcttaagcg cagcgccatt ttagcaacgc
     2941 agaagcccgg cgccgggaag cctcagctcg cctgaaggca ggtcccctct gacgcctccg
     3001 ggagcccagg tttcccagag tccttgggac gcagcgacga gttgtgctgc tatcttagct
     3061 gtccttatag gctggccatt ccaggtggtg gtatttagat aaaaccactc aaactctgca
     3121 gtttggtctt ggggtttgga ggaaagcttt tatttttctt cctgctccgg ttcagaaggt
     3181 ctgaagctca tacctaaccg ggcataacac agaatctgca aaacaaaaac ccctaaaaaa
     3241 gcagacccag agcagtgtaa acacttctgg gtgtgtccct gactggctgc ccaaggtctc
     3301 tgtgtcttcg gagacaaagc cattcgctta gttggtctac tttaaaaggc cacttgaact
     3361 cgctttccat ggcgatttgc cttgtgagca ctttcaggag agcctggaag ctgaaaaacg
     3421 gtagaaaaat ttccgtgcgg gccgtggggg gctggcggca actggggggc cgcagatcag
     3481 agtgggccac tggcagccaa cggcccccgg ggctcaggcg gggagcagct ctgtggtgtg
     3541 ggattgaggc gttttccaag agtgggtttt cacgtttcta agatttccca agcagacagc
     3601 ccgtgctgct ccgatttctc gaacaaaaaa gcaaaacgtg tggctgtctt gggagcaagt
     3661 cgcaggactg caagcagttg ggggagaaag tccgccattt tgccacttct caaccgtccc
     3721 tgcaaggctg gggctcagtt gcgtaatgga aagtaaagcc ctgaactatc acactttaat
     3781 cttccttcaa aaggtggtaa actataccta ctgtccctca agagaacaca agtgctttaa
     3841 gaggtatttt aaaagttccg ggggttttgt gaggtgtttg atgacccgtt taaaatatga
     3901 tttccatgtt tcttttgtct aaagtttgca gctcaaatct ttccacacgc tagtaattta
     3961 agtatttctg catgtgtagt ttgcattcaa gttccataag ctgttaagaa aaatctagaa
     4021 aagtaaaact agaacctatt tttaaccgaa gaactacttt ttgcctccct cacaaaggcg
     4081 gcggaaggtg atcgaattcc ggtgatgcga gttgttctcc gtctataaat acgcctcgcc
     4141 cgagctgtgc ggtaggcatt gaggcagcca gcgcaggggc ttctgctgag ggggcaggcg
     4201 gagcttgagg aaaccgcaga tgagtttttt tctctttgaa agatagagat taatacaact
     4261 acttaaaaaa tatagtcaat aggttactaa gatattgctt agcgttaagt ttttaacgta
     4321 attttaatag cttaagattt taagagaaaa tatgaagact tagaagagta gcatgaggaa
     4381 ggaaaagata aaaggtttct aaaacatgac ggaggttgag atgaagcttc ttcatggagt
     4441 aaaaaatgta tttaaaagaa aattgagaga aaggactaca gagccccgaa ttaataccaa
     4501 tagaagggca atgcttttag attaaaatga aggtgactta aacagcttaa agtttagttt
     4561 aaaagttgta ggtgattaaa ataatttgaa ggcgatcttt taaaaagaga ttaaactgaa
     4621 ggtgattaaa agaccttgaa atccatgacg cagggagaat tgcgtcattt aaagcctagt
     4681 taacgcattt actaaacgca gacgaaaatg gaaagattaa ttgggagtgg taggatgaaa
     4741 caatttggag aagatagaag tttgaagtgg aaaactggaa gacagaagta cgggaaggcg
     4801 aagaaaagaa tagagaagat agggaaatta gaagataaaa acatactttt agaagaaaaa
     4861 agataaattt aaacctgaaa agtaggaagc agaagaaaaa agacaagcta ggaaacaaaa
     4921 agctaagggc aaaatgtaca aacttagaag aaaattggaa gatagaaaca agatagaaaa
     4981 tgaaaatatt gtcaagagtt tcagatagaa aatgaaaaac aagctaagac aagtattgga
     5041 gaagtataga agatagaaaa atataaagcc aaaaattgga taaaatagca ctgaaaaaat
     5101 gaggaaatta ttggtaacca atttatttta aaagcccatc aatttaattt ctggtggtgc
     5161 agaagttaga aggtaaagct tgagaagatg agggtgttta cgtagaccag aaccaattta
     5221 gaagaatact tgaagctaga aggggaagtt ggttaaaaat cacatcaaaa agctactaaa
     5281 aggactggtg taatttaaaa aaaactaagg cagaaggctt ttggaagagt tagaagaatt
     5341 tggaaggcct taaatatagt agcttagttt gaaaaatgtg aaggactttc gtaacggaag
     5401 taattcaaga tcaagagtaa ttaccaactt aatgtttttg cattggactt tgagttaaga
     5461 ttatttttta aatcctgagg actagcatta attgacagct gacccaggtg ctacacagaa
     5521 gtggattcag tgagtctagg aagacagcag cagacaggat tccaggaacc agtgtttgat
     5581 gaagctagga ctgaggagca agcgagcaag cagcagttcg tggtgaagat aggaaaagag
     5641 tccaggagcc agtgcgattt ggtgaaggaa gctaggaaga aggaaggagc gctaacgatt
     5701 tggtggtgaa gctaggaaaa aggattccag gaaggagcga gtgcaatttg gtgatgaagg
     5761 tagcaggcgg cttggcttgg caaccacacg gaggaggcga gcaggcgttg tgcgtagagg
     5821 atcctagacc agcatgccag tgtgccaagg ccacagggaa agcgagtggt tggtaaaaat
     5881 ccgtgaggtc ggcaatatgt tgtttttctg gaacttactt atggtaacct tttatttatt
     5941 ttctaatata atgggggagt ttcgtactga ggtgtaaagg gatttatatg gggacgtagg
     6001 ccgatttccg ggtgttgtag gtttctcttt ttcaggctta tactcatgaa tcttgtctga
     6061 agcttttgag ggcagactgc caagtcctgg agaaatagta gatggcaagt ttgtgggttt
     6121 tttttttttt acacgaattt gaggaaaacc aaatgaattt gatagccaaa ttgagacaat
     6181 ttcagcaaat ctgtaagcag tttgtatgtt tagttggggt aatgaagtat ttcagttttg
     6241 tgaatagatg acctgttttt acttcctcac cctgaattcg ttttgtaaat gtagagtttg
     6301 gatgtgtaac tgaggcgggg gggagttttc agtatttttt tttgtggggg tgggggcaaa
     6361 atatgttttc agttcttttt cccttaggtc tgtctagaat cctaaaggca aatgactcaa
     6421 ggtgtaacag aaaacaagaa aatccaatat caggataatc agaccaccac aggtttacag
     6481 tttatagaaa ctagagcagt tctcacgttg aggtctgtgg aagagatgtc cattggagaa
     6541 atggctggta gttactcttt tttcccccca cccccttaat cagactttaa aagtgcttaa
     6601 ccccttaaac ttgttatttt ttacttgaag cattttggga tggtcttaac agggaagaga
     6661 gagggtgggg gagaaaatgt ttttttctaa gattttccac agatgctata gtactattga
     6721 caaactgggt tagagaagga gtgtaccgct gtgctgttgg cacgaacacc ttcagggact
     6781 ggagctgctt ttatccttgg aagagtattc ccagttgaag ctgaaaagta cagcacagtg
     6841 cagctttggt tcatattcag tcatctcagg agaacttcag aagagcttga gtaggccaaa
     6901 tgttgaagtt aagttttcca ataatgtgac ttcttaaaag ttttattaaa ggggaggggc
     6961 aaatattggc aattagttgg cagtggcctg ttacggttgg gattggtggg gtgggtttag
     7021 gtaattgttt agtttatgat tgcagataaa ctcatgccag agaacttaaa gtcttagaat
     7081 ggaaaaagta aaggaatatc aacttccaag ttggcaagta actcccaatg atttagtttt
     7141 tttcccccca gtttgaattg ggaagctggg ggaagttaaa tatgagccac tgggtgtacc
     7201 agtgcattaa tttgggcaag gaaagtgtca taatttgata ctgtatctgt tttccttcaa
     7261 agtatagagc ttttggggaa ggaaagtatt gaactggggg ttggtctggc ctactgggct
     7321 gacattaact acaattatgg gaaatgcaaa agttgtttgg atatggtagt gtgtggttct
     7381 cttttggaat ttttttcagg tgatttaata ataatttaaa actactatag aaactgcaga
     7441 gcaaaggaag tggcttaatg atcctgaagg gatttcttct gatggtagct tttgtattat
     7501 caagtaagat tctattttca gttgtgtgta tgcaagtttt tttttagtgt aggagaaata
     7561 cttttccatt gtttaactgc aaaacaagat gttaaggtat gcttcaaaaa ttttgtaaat
     7621 tgtttatttt aaacttatct gtttgtaaat tgtaactgat taagaattgt gatagttcag
     7681 cttgaatgtc tcttagaggg tgggcttttg ttgatgaggg aggggaaact tttttttttt
     7741 ctatagactt ttttcagata acatcttctg agtcataacc agcctggcag tatgatggcc
     7801 tagatgcaga gaaaacagct ccttggtgaa ttgataagta aaggcagaaa agattatatg
     7861 tcatacctcc attggggaat aagcataacc ctgagattct tactactgat gagaacatta
     7921 tctgcatatg ccaaaaaatt ttaagcaaat gaaagctacc aatttaaagt tacggaatct
     7981 accattttaa agttaattgc ttgtcaagct ataaccacaa aaataatgaa ttgatgagaa
     8041 atacaatgaa gaggcaatgt ccatctcaaa atactgcttt tacaaaagca gaataaaagc
     8101 gaaaagaaat gaaaatgtta cactacatta atcctggaat aaaagaagcc gaaataaatg
     8161 agagatgagt tgggatcaag tggattgagg aggctgtgct gtgtgccaat gtttcgtttg
     8221 cctcagacag gtatctcttc gttatcagaa gagttgcttc atttcatctg ggagcagaaa
     8281 acagcaggca gctgttaaca gataagttta acttgcatct gcagtattgc atgttaggga
     8341 taagtgctta tttttaagag ctgtggagtt cttaaatatc aaccatggca ctttctcctg
     8401 accccttccc taggggattt caggattgag aaatttttcc atcgagcctt tttaaaattg
     8461 taggacttgt tcctgtgggc ttcagtgatg ggatagtaca cttcactcag aggcatttgc
     8521 atctttaaat aatttcttaa aagcctctaa agtgatcagt gccttgatgc caactaagga
     8581 aatttgttta gcattgaatc tctgaaggct ctatgaaagg aatagcatga tgtgctgtta
     8641 gaatcagatg ttactgctaa aatttacatg ttgtgatgta aattgtgtag aaaaccatta
     8701 aatcattcaa aataataaac tatttttatt agagaatgta tacttttaga aagctgtctc
     8761 cttatttaaa taaaatagtg tttgtctgta gttcagtgtt ggggcaatct tgggggggat
     8821 tcttctctaa tctttcagaa actttgtctg cgaacactct ttaatggacc agatcaggat
     8881 ttgagcggaa gaacgaatgt aactttaagg caggaaagac aaattttatt cttcataaag
     8941 tgatgagcat ataataattc caggcacatg gcaatagagg ccctctaaat aaggaataaa
     9001 taacctctta gacaggtggg agattatgat cagagtaaaa ggtaattaca cattttattt
     9061 ccagaaagtc aggggtctat aaattgacag tgattagagt aatacttttt cacatttcca
     9121 aagtttgcat gttaacttta aatgcttaca atcttagagt ggtaggcaat gttttacact
     9181 attgacctta tatagggaag ggagggggtg cctgtggggt tttaaagaat tttcctttgc
     9241 agaggcattt catccttcat gaagccattc aggattttga attgcatatg agtgcttggc
     9301 tcttccttct gttctagtga gtgtatgaga ccttgcagtg agtttatcag catactcaaa
     9361 atttttttcc tggaatttgg agggatggga ggagggggtg gggcttactt gttgtagctt
     9421 ttttttttta cagacttcac agagaatgca gttgtcttga cttcaggttt gtctgttctg
     9481 ttggcaagta aatgcagtac tgttctgatc ccgctgctat tagaatgcat tgtgaaacga
     9541 ctggagtatg attaaaagtt gtgttcccca atgcttggag tagtgattgt tgaaggaaaa
     9601 aatccagctg agtgataaag gctgagtgtt gaggaaattt ctgcagtttt aagcagtcgt
     9661 atttgtgatt gaagctgagt acattttgct ggtgtatttt taggtaaaat gctttttgtt
     9721 catttctggt ggtgggaggg gactgaagcc tttagtcttt tccagatgca accttaaaat
     9781 cagtgacaag aaacattcca aacaagcaac agtcttcaag aaattaaact ggcaagtgga
     9841 aatgtttaaa cagttcagtg atctttagtg cattgtttat gtgtgggttt ctctctcccc
     9901 tcccttggtc ttaattctta catgcaggaa cactcagcag acacacgtat gcgaagggcc
     9961 agagaagcca gacccagtaa gaaaaaatag cctatttact ttaaataaac caaacattcc
    10021 attttaaatg tggggattgg gaaccactag ttctttcaga tggtattctt cagactatag
    10081 aaggagcttc cagttgaatt caccagtgga caaaatgagg aaaacaggtg aacaagcttt
    10141 ttctgtattt acatacaaag tcagatcagt tatgggacaa tagtattgaa tagatttcag
    10201 ctttatgctg gagtaactgg catgtgagca aactgtgttg gcgtgggggt ggaggggtga
    10261 ggtgggcgct aagccttttt ttaagatttt tcaggtaccc ctcactaaag gcaccgaagg
    10321 cttaaagtag gacaaccatg gagccttcct gtggcaggag agacaacaaa gcgctattat
    10381 cctaaggtca agagaagtgt cagcctcacc tgatttttat tagtaatgag gacttgcctc
    10441 aactccctct ttctggagtg aagcatccga aggaatgctt gaagtacccc tgggcttctc
    10501 ttaacattta agcaagctgt ttttatagca gctcttaata ataaagccca aatctcaagc
    10561 ggtgcttgaa ggggagggaa agggggaaag cgggcagcca cttttcccta gcttttccag
    10621 aagcctgtta aaagcaaggt ctccccacaa gcaacttctc tgccacatcg ccaccccgtg
    10681 ccttttgatc tagcacagac ccttcacccc tcacctcgat gcagccagta gcttggatcc
    10741 ttgtgggcat gatccataat cggtttcaag gtaacgatgg tgtcgaggtc tttggtgggt
    10801 tgaactatgt tagaaaaggc cattaatttg cctgcaaatt gttaacagaa gggtattaaa
    10861 accacagcta agtagctcta ttataatact tatccagtga ctaaaaccaa cttaaaccag
    10921 taagtggaga aataacatgt tcaagaactg taatgctggg tgggaacatg taacttgtag
    10981 actggagaag ataggcattt gagtggctga gagggctttt gggtgggaat gcaaaaattc
    11041 tctgctaaga ctttttcagg tgaacataac agacttggcc aagctagcat cttagcggaa
    11101 gctgatctcc aatgctcttc agtagggtca tgaaggtttt tcttttcctg agaaaacaac
    11161 acgtattgtt ttctcaggtt ttgctttttg gcctttttct agcttaaaaa aaaaaaaagc
    11221 aaaagatgct ggtggttggc actcctggtt tccaggacgg ggttcagatc cctgcggcgt
    11281 ctttgctttg actactaatc tgtcttcagg actctttctg tatttctcct tttctctgca
    11341 ggtgctagtt cttggagttt tggggaggtg ggagataaca gcacaatatc tttgaactat
    11401 atacatcctt gatgtataat ttgtcaggag cttgacttga ttgtatattc atatttacac
    11461 gagaacctaa tataactgcc ttgtcttttt caggtaatag cctgcagctg gtgttttgag
    11521 aagccctact gctgaaaact taacaatttt gtgtaataaa aatggagaag ctctaaattg
    11581 ttgtggttct tttgtgaata aaaaaatctt gattggggaa aaaaaaactc gagggccgcg
    11641 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
    11701 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
    11761 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
    11821 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
    11881 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
    11941 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
    12001 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
    12061 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
    12121 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
    12181 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga
    12241 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
    12301 cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt
    12361 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
    12421 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
    12481 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc
    12541 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat
    12601 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
    12661 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag
    12721 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
    12781 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg
    12841 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca
    12901 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg
    12961 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta
    13021 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    13081 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    13141 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    13201 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
    13261 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    13321 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    13381 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    13441 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    13501 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    13561 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct
    13621 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    13681 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    13741 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    13801 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    13861 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    13921 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    13981 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
    14041 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    14101 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
    14161 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
    14221 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    14281 taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag
    14341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    14401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    14461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
    14521 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
    14581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    14641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    14701 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    14761 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    14821 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
    14881 ttgcagagac caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
    14941 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    15001 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    15061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    15121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    15181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    15241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    15301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    15361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    15421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    15481 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    15541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    15601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    15661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    15721 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    15781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    15841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    15901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    15961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    16021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    16081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    16141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    16201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    16261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    16321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    16381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    16441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    16501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    16561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    16621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    16681 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    16741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    16801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    16861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    16921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    16981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    17041 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    17101 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    17161 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    17221 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    17281 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    17341 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    17401 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    17461 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    17521 gtgccaagct g
//
