LOCUS       Exported               12878 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    R4pGWB601_UBQ10p-Turbo-NES-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID.
  JOURNAL   Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
  PUBMED    31535972
REFERENCE   2  (bases 1 to 12878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2361..2385
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2399..2440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2447..3403
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             3410..3436
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3467..4186
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3512..3533)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3773..3792)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4120..4141
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4209..4233)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4265..4517
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4550..4566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4550..4566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4563..4585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4574..4590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4598..4628)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4643..4664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      4700..4952
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5112..5663)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5715..5894)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6400..6424
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6945..7736
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7982..8570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8471..8490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8724..8741
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8756..8896
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8822..8841)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8982..9004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9240..9434
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9500..10573)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11002..11631)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tggctccgaa ttcgccctta
      361 gtctagctca acagagcttt taacccaaat tggtacaata gaatacaact ttagatcata
      421 attctcaaaa gaaagagatt ccttagctat tctatctgcc actccatttc cttctcggct
      481 tgtatgcaca agcataaaat cctcaaactt gctaagtaga tactttatgt cttggataat
      541 tggattgaga cttgacaagc ataactttca tgtaaccaaa gacacaagtt gctgagaatc
      601 cacctcaaaa atgatcttcc tataattgaa tcgggataat gacagcacag cccatctaag
      661 agcctccact tctacttcca gcacgcttct tacttttacc acagctcttg cacctaacca
      721 taacaccttc cctgtatgat cgcgaagcac ccaccctaag ccacatttta atccttctgt
      781 tggccatgcc ccatcaaagt tgcacttaac ccaagattgt ggtggagctt cccatgtttc
      841 tcgtctgtcc cgacggtgtt gtggttggtg ctttccttac attctgagcc tctttccttc
      901 taatccactc atctgcatct tcttgtgtcc ttactaatac ctcattggtt ccaaattccc
      961 tccctttaag caccagctcg tttctgttct tccacagcct cccaagtatc caagggacta
     1021 aagcctccac attcttcaga tcaggatatt cttgtttaag atgttgaact ctatggaggt
     1081 ttgtatgaac tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa
     1141 agaatgtttt gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat
     1201 tggactgaac acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga
     1261 attaaataac aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt
     1321 caccaacttg atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca
     1381 ataacactaa aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt
     1441 tacttttcca agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa
     1501 aaaacaaaaa ggaaaagaaa taaagcacga agaattctag aaaatacgaa atacgcttca
     1561 atgcagtggg acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa
     1621 ataaaacgat aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga
     1681 tcttatgacg accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag
     1741 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     1801 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     1861 gtgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     1921 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
     1981 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     2041 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     2101 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     2161 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     2221 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat
     2281 ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt
     2341 aacagaaggg cgaattcgac ccaagtttgt acaaaaaagc aggctccgcg gccgcatggg
     2401 caagcccatc cccaaccccc tgctgggcct ggacagcacc gctagcaaag acaatactgt
     2461 gcctctgaag ctgatcgctc tcctggctaa tggcgagttc catagtggcg aacagctggg
     2521 agaaaccctg ggcatgtcca gggccgctat caacaagcac attcagactc tgcgcgactg
     2581 gggcgtggac gtgttcaccg tgcccggaaa gggctactct ctgcccgagc ctatcccgct
     2641 gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt
     2701 ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc
     2761 ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc
     2821 ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat
     2881 cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga
     2941 caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat
     3001 cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat
     3061 taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca
     3121 ggaagcaggg attaacctgg acaggaatac tctggccgct acgctgatcc gagagctgcg
     3181 ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgccac ggtgggagaa
     3241 gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg
     3301 gattagccgg gggattgata aacagggagc cctgctgctg gaacaggacg gagttatcaa
     3361 accctggatg ggcggagaaa tcagtctgcg gtctgccgaa aagctgcagc tgcctcccct
     3421 ggagcgcctg accctggacg gaggcggtgg aagcggcgga ggttccatgg tgagcaaggg
     3481 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3541 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3601 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     3661 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     3721 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3781 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3841 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3901 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3961 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     4021 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     4081 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     4141 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagggc ccaagggtgg
     4201 gcgcgccgac ccagctttct tgtacaaagt ggttgataac agcgcttaga gctcgaattt
     4261 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     4321 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     4381 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     4441 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     4501 atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg tcatagctgt
     4561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     4681 cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     4741 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     4801 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     4861 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     4921 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgagctggg gacgtcccca
     4981 gctcggtacc ccctcctggg cacggtcgta cgcgatccgc cgcggttcca cctcaccatt
     5041 gcgggtatgc cagttgagtt cgatgagttc ccgtaccagc tccaactcgg gcgatgccac
     5101 gcgcactccg ttcagatctc ggtgacgggc aggaccggac ggggcggtac cggcaggctg
     5161 aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc
     5221 agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc
     5281 agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt cagcaggtgg
     5341 gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta cacggtcgac
     5401 tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc gatgccggcg
     5461 acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg gacgaggtcg
     5521 tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg
     5581 tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg gcggatgtcg
     5641 gccgggcgtc gttctgggct catatcttat tgccccccgg gatcagatct ggattgagag
     5701 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     5761 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     5821 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt
     5881 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
     5941 gtcataacgt gactccctta attctccgct catgatcttg atcccctgcg ccatcagatc
     6001 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     6061 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     6121 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     6181 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     6241 gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagctgat
     6301 ccgtcgagat taatagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa
     6361 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     6421 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     6481 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     6541 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     6601 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6661 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     6721 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     6781 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     6841 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     6901 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     6961 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     7021 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     7081 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     7141 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     7201 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     7261 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     7321 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     7381 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     7441 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     7501 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     7561 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     7621 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     7681 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     7741 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     7801 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     7861 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     7921 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7981 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8041 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8101 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     8161 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8221 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8281 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8341 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8401 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8461 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8521 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8581 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8641 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8701 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     8761 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     8821 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     8881 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8941 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     9001 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     9061 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     9121 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     9181 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     9241 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     9301 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     9361 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     9421 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     9481 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     9541 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     9601 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     9661 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     9721 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     9781 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     9841 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     9901 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     9961 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
    10021 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
    10081 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
    10141 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
    10201 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
    10261 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
    10321 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
    10381 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
    10441 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
    10501 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    10561 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
    10621 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
    10681 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
    10741 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
    10801 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
    10861 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
    10921 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
    10981 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
    11041 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    11101 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    11161 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    11221 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    11281 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    11341 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    11401 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    11461 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    11521 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    11581 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    11641 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    11701 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    11761 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    11821 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    11881 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    11941 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    12001 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    12061 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    12121 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    12181 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    12241 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    12301 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    12361 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    12421 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    12481 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    12541 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    12601 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    12661 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    12721 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    12781 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    12841 gttggcatgc acatacaaat ggacgaacgg ataaacct
//
