LOCUS Exported 2167 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION _-factor pheromone. ACCESSION . VERSION . KEYWORDS pWS1837 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2167) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2167) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2167 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..279 /codon_start=1 /gene="MF-alpha-1" /product="N-terminal secretion signal from S. cerevisiae alpha-factor" /label=-alpha-factor secretion signal /note="Cleavage by the Kex2 protease occurs after the dibasic KR sequence. The EA dipeptides are then removed by dipeptidyl aminopeptidase A." /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLE GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKREAEA" terminator 534..568 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(639..1298) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1213..1232 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1480..2068 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1969..1988 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatgagatt tccttcaatt tttactgcag ttttattcgc agcatcctcc 61 gcattagctg ctccagtcaa cactacaaca gaagatgaaa cggcacaaat tccggctgaa 121 gctgtcatcg gttacttaga tttagaaggg gatttcgatg ttgctgtttt gccattttcc 181 aacagcacaa ataacgggtt attgtttata aatactacta ttgccagcat tgctgctaaa 241 gaagaagggg tatctttgga taaaagagag gctgaagctt ggcattggtt gcaactaaaa 301 cctggccaac caatgtacaa gagagaagcc gaagctgaag cttggcattg gctgcaacta 361 aagcctggcc aaccaatgta caaaagagaa gccgacgctg aagcttggca ttggctgcaa 421 ctaaagcctg gccaaccaat gtacaaaaga gaagccgacg ctgaagcttg gcattggttg 481 cagttaaaac ccggccaacc aatgtactaa ctcgagtggc tgagaccaga ccaataaaaa 541 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg 601 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca 661 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 721 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 781 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 841 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa 901 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 961 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 1021 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga 1081 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 1141 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 1201 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 1261 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 1321 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 1381 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca 1441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1621 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2101 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2161 taaccgt //