LOCUS       Exported                3503 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express gene of interest under human Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    AV0-hSyn-SV40pA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  TITLE     Oka Lab plasmids
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   44..184
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   55..184
                     /label=AAV2 ITR
     promoter        212..659
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     polyA_signal    724..805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(761..780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   820..949
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(1011..1599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1091..1110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1770..2630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2393..2412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2631..2735)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2803..2821
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2859..2881)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2981..3000
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3017..3472)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3154..3175)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3366..3385
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gcccctgcag gcagctgcgc
       61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
      121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
      181 tcctgcggcc ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga
      241 ggcggggtgg gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc
      301 ccattcccca aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag
      361 gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg
      421 cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa
      481 ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc
      541 acgcgaggcg cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact
      601 cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt
      661 cgagaaggta ccggatccgc tagcgcggcc gctcgagaat tctatcgata agtaagtgac
      721 tagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      781 acaaataaag catttttttc actgcattct agtggccgca ggaaccccta gtgatggagt
      841 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
      901 gacgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcagga catgtgagca
      961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcacct tatccggtaa ctatcgtctt
     1321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1441 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2161 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2641 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2701 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2761 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2821 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2881 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2941 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3001 ctgagagtgc accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     3061 tgttaaatca gctcattttt taaccaatag accgaaatcg gcaaaatccc ttataaatca
     3121 aaagaatagc ccgagataga gttgagtgtt gttccagttt ggaacaagag tccactatta
     3181 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3241 cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3301 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3361 aaggaaggga agaaagcgaa aggagcgggc gctaaggcgc tggcaagtgt agcggtcacg
     3421 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt
     3481 tgctttgacg tatgcggtgt gaa
//
