LOCUS       Exported                9519 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Tuning expression of PD-1.
ACCESSION   .
VERSION     .
KEYWORDS    PD-1-miSFIT-4x
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre 
            V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, 
            Milne TA, Cerundolo V, Fulga TA
  TITLE     Precise tuning of gene expression levels in mammalian cells.
  JOURNAL   Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y.
  PUBMED    30778069
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(760..1620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1751..2206)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1888..1909)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2100..2119
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2333..2355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2347..2364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2348..2364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2374..2393
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2374..2392
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2413..2548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2467..2486)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    2588..2709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2599..2618)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2781..3014
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3101..3689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3207..3218
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3616..3636
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(4693..5203)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4756..4774)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5231..5269
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5266..5290
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    6418..6552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6689..6806
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7073..7117)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7302..7535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8028..8153
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8200..8380
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8395..8598)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8424..8444)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8599..8978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(9079..9099)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9079..9097)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9118..9134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9118..9134)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9131..9153)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9142..9158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9166..9196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9211..9232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9349..9366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     1861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     2221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2281 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2341 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     2401 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     2461 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     2521 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     2581 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2641 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2701 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     2761 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
     2821 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     2881 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     2941 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     3001 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     3061 gtaagtcatt ggtcttaaag gtaccgagct cgaattccag gcggggaggc ggcccaaagg
     3121 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     3181 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     3241 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
     3301 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     3361 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     3421 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     3481 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     3541 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3601 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3661 tcacaaattt tgtaatccag aggttgattg tcgactctag actcgactct agaggatccc
     3721 cctgttccac ctcttgaagg ctatgtaggc cacaaggccc acaaccacag cagccaggat
     3781 ggagcaatag acagggatga ggttgtcggt ggtgcctcgg gtcaccacgg gctgggagct
     3841 gcccatcact gtggtcacca cacctgccac cgtgctggct atgaggtctt gttctggagg
     3901 tgcctcaggc tcctgggtgc tgggggctgt gctgtccgag ccctctgggg gtgtggaccg
     3961 tgtaatccaa cggccaggga tctcctcgca ctcggcgtcg gcccagcgtg tgcactcgcg
     4021 gagctggcgc tcggtgtcct cgcacacggt gcagggcagg cacgggtcca cgtggttggc
     4081 ctcgtcggaa tacgtgccgt cggggcactc ctcgcacacg gtgttctgct tgtcctggca
     4141 ggagaacacg aggcccgagc ccgcctcgca cacgcggcac gcctcgcagc gcccagtcgt
     4201 ctcatcctgg tagtagccgt aggcgcagcg gcacacggcg tcgtcggcct ccacgcacgg
     4261 cgccgacatg ctctggagcc ccacgcactc ggtgcacggc ttgcacggct cggtcgcgct
     4321 caccacgtcg gagaacgtca cgctgtccag gcagggctca cacacggtct ggttggctcc
     4381 acaaggctgg gccacaccct cgcccaggtt gcaggctttg cagcactcac cgctgtgtgt
     4441 gtacaggcct gtggggcatg cctccttggc acctccaagg gacaccccca gaagcagcaa
     4501 cagcagcagg cgcggcccgt ccatggcgcg gccggtggca cctgccccca tcgcccgcct
     4561 cccgcggcag cgctcgactt ccagctcggt ccgctttgcg gactgatggg gctgcgctgc
     4621 gctgcgctcc agcgcccccc ctgcccgccg gagctggccg cggcccgaat tcctgcagcc
     4681 cgggggatcc ccctggggag agaggtcggt gattcggtca acgagggagc cgactgccga
     4741 cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg ggcaggaaca gggcccacac
     4801 taccgcccca caccccgcct cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc
     4861 tgctgagcag ccgctattgg ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg
     4921 gcgctgtccg tctgcgaggg tactagtgag acgtgcggct tccgtttgtc acgtccggca
     4981 cgccgcgaac cgcaaggaac cttcccgact taggggcgga gcaggaagcg tcgccggggg
     5041 gcccacaagg gtagcggcga agatccgggt gacgctgcga acggacgtga agaatgtgcg
     5101 agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg
     5161 cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac cccgtggaat tcgatatcaa
     5221 gcttctcgag ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     5281 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggag
     5341 ctcctgcagg tgccgccatg cagatcccac aggcgccctg gccagtcgtc tgggcggtgc
     5401 tacaactggg ctggcggcca ggatggttct tagactcccc agacaggccc tggaaccccc
     5461 ccaccttctc cccagccctg ctcgtggtga ccgaagggga caacgccacc ttcacctgca
     5521 gcttctccaa cacatcggag agcttcgtgc taaactggta ccgcatgagc cccagcaacc
     5581 agacggacaa gctggccgcc ttccccgagg accgcagcca gcccggccag gactgccgct
     5641 tccgtgtcac acaactgccc aacgggcgtg acttccacat gagcgtggtc agggcccggc
     5701 gcaatgacag cggcacctac ctctgtgggg ccatctccct ggcccccaag gcgcagatca
     5761 aagagagcct gcgggcagag ctcagggtga cagagagaag ggcagaagtg cccacagccc
     5821 accccagccc ctcacccagg ccagccggcc agttccaaac cctggtggtt ggtgtcgtgg
     5881 gcggcctgct gggcagcctg gtgctgctag tctgggtcct ggccgtcatc tgctcccggg
     5941 ccgcacgagg gacaatagga gccaggcgca ccggccagcc cctgaaggag gacccctcag
     6001 ccgtgcctgt gttctctgtg gactatgggg agctggattt ccagtggcga gagaagaccc
     6061 cggagccccc cgtgccctgt gtccctgagc agacggagta tgccaccatt gtctttccta
     6121 gcggaatggg cacctcatcc cccgcccgca ggggctcagc cgacggccct cggagtgccc
     6181 agccactgag gcctgaggat ggacactgct cttggcccct ctgaggggta gcacgtgagt
     6241 gctagcctac ctgcactgta agcactttgt atctacctgc actgtaagca ctttgtatct
     6301 acctgcactg taagcacttt gtatctacct gcactgtaag cactttgacc ggtactagct
     6361 tagtcatgca ccgggctgca ggaattcgat gacaggcgca gcttccgagg gatttgagat
     6421 ccagacatga taagatacat tgatgagttt ggacaaacca aaactagaat gcagtgaaaa
     6481 aaatgcctta tttgtgaaat ttgtgatgct attgccttat ttgtaaccat tataagctgc
     6541 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     6601 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag
     6661 atctctcgag gctagtctcg tgatcgataa aattttgaat ttttgtaatt tgtttttgta
     6721 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
     6781 ccccaatccc cccttttctt ttaaaagtta accgataccg tcgagatccg ttcactaatc
     6841 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     6901 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     6961 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     7021 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     7081 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     7141 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     7201 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     7261 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     7321 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     7381 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     7441 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     7501 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     7561 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     7621 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     7681 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     7741 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     7801 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     7861 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     7921 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     7981 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     8041 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     8101 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     8161 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     8221 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     8281 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     8341 agagctccca ggctcagatc tggtctaacc agagagaccc cggttcacta aacgagctct
     8401 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     8461 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     8521 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     8581 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     8641 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     8701 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     8761 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     8821 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     8881 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     8941 aattgattac tattaataac tagtcaataa tcaatgtcaa catggcggta atgttggaca
     9001 tgagccaata taaatgtaca tattatgata tggatacaac gtatgcaatg gccaagcttg
     9061 cagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     9121 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9181 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     9241 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9301 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9361 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9421 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9481 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgtt
//
