LOCUS Exported 6141 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBabe-GFP-IKBalpha-wt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6141) AUTHORS Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC TITLE Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. JOURNAL Cell. 2007 Jun 15. 129(6):1065-1079. PUBMED 17574021 REFERENCE 2 (bases 1 to 6141) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6141 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 23..492 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 557..756 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 957..1373 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1303..1325 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1341..1357 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(2389..2409) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2394..2723 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2574..2709 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2636..2655 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(2732..2748) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2733..2749) /label=pBluescriptKS /note="For pBluescript vector" CDS 2754..3473 /codon_start=1 /product="humanized Renilla green fluorescent protein" /label=hrGFP /note="mammalian codon-optimized" /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI AQLTSLGKPLGSLHEWV" LTR 3650..4119 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4249..4266) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4420..5008) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4500..4519) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5168..6028) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5791..5810 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6029..6133) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca gcctgaatat gggccaaaca ggatatctgt ggtaagcagt 61 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata 121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg 181 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg 241 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc 301 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct 361 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc 421 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc 481 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga 541 cccaccaccg ggaggcaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt 601 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc 661 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca 721 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga 781 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt 841 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca 901 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc 961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg 1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat 1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc 1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca 1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc 1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt 1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggccgga 1381 tctctcgagg ttagcttatg ttccaggcgg ccgagcgtcc ccaggagtgg gccatggagg 1441 gcccccgcga cgggctgaag aaggagcggc tactggacga ccgccacgac agcggcctgg 1501 actccatgaa agacgaggag tacgagcaga tggtcaagga gctgcaggag atccgcctcg 1561 agccgcagga ggtgccgcgc ggctcggagc cctggaagca gcagctcacc gaggacgggg 1621 actcgttcct gcacttggcc atcatccatg aagaaaaggc actgaccatg gaagtgatcc 1681 gccaggtgaa gggagacctg gccttcctca acttccagaa caacctgcag cagactccac 1741 tccacttggc tgtgatcacc aaccagccag aaattgctga ggcacttctg ggagctggct 1801 gtgatcctga gctccgagac tttcgaggaa atacccccct acaccttgcc tgtgagcagg 1861 gctgcctggc cagcgtggga gtcctgactc agtcctgcac caccccgcac ctccactcca 1921 tcctgaaggc taccaactac aatggccaca cgtgtctaca cttagcctct atccatggct 1981 acctgggcat cgtggagctt ttggtgtcct tgggtgctga tgtcaatgct caggagccct 2041 gtaatggccg gactgccctt cacctcgcag tggacctgca aaatcctgac ctggtgtcac 2101 tcctgttgaa gtgtggggct gatgtcaaca gagttaccta ccagggctat tctccctacc 2161 agctcacctg gggccgccca agcacccgga tacagcagca gctgggccag ctgacactag 2221 aaaaccttca gatgctgcca gagagtgagg atgaggagag ctatgacaca gagtcagagt 2281 tcacggagtt cacagaggac gagctgccct atgatgactg tgtgtttgga ggccagcgtc 2341 tgacgttatg aggatcaacg aattcgccag cacagtggtc gaccctgtgg aatgtgtgtc 2401 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2461 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2521 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 2581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 2641 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 2701 ttggaggcct aggcttttgc aaaaagctta tcgataccgt cgacctcgag accatggtga 2761 gcaagcagat cctgaagaac accggcctgc aggagatcat gagcttcaag gtgaacctgg 2821 agggcgtggt gaacaaccac gtgttcacca tggagggctg cggcaagggc aacatcctgt 2881 tcggcaacca gctggtgcag atccgcgtga ccaagggcgc ccccctgccc ttcgccttcg 2941 acatcctgag ccccgccttc cagtacggca accgcacctt caccaagtac cccgaggaca 3001 tcagcgactt cttcatccag agcttccccg ccggcttcgt gtacgagcgc accctgcgct 3061 acgaggacgg cggcctggtg gagatccgca gcgacatcaa cctgatcgag gagatgttcg 3121 tgtaccgcgt ggagtacaag ggccgcaact tccccaacga cggccccgtg atgaagaaga 3181 ccatcaccgg cctgcagccc agcttcgagg tggtgtacat gaacgacggc gtgctggtgg 3241 gccaggtgat cctggtgtac cgcctgaaca gcggcaagtt ctacagctgc cacatgcgca 3301 ccctgatgaa gagcaagggc gtggtgaagg acttccccga gtaccacttc atccagcacc 3361 gcctggagaa gacctacgtg gaggacggcg gcttcgtgga gcagcacgag accgccatcg 3421 cccagctgac cagcctgggc aagcccctgg gcagcctgca cgagtgggtg taatagggta 3481 ccttcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc 3541 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca 3601 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg 3661 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga 3721 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg 3781 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag 3841 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat 3901 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca 3961 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat 4021 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct 4081 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt 4141 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg 4201 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 4261 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4321 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 4501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 4561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 4621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4861 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5101 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt 5161 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 5221 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 5281 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 5341 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 5401 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 5461 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 5521 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 5581 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 5641 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 5701 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 5761 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 5821 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 5881 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 5941 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 6001 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 6061 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 6121 taggggttcc gcgcacattt c //