LOCUS       Exported                4098 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express gene of interest in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    AV0hSynWS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  TITLE     Oka Lab plasmids
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(35..482)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     repeat_region   510..650
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   510..639
                     /label=AAV2 ITR
     rep_origin      725..1180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(812..831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1022..1043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1197..1216)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1316..1338
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1376..1394)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1462..1566
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1567..2427
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1785..1804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2598..3186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3087..3106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3248..3377
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    3392..3473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3417..3436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    3494..4082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3600..3611
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4009..4029
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agcggccgcg ctagcggatc cggtaccttc tcgactgcgc tctcaggcac gacacgactc
       61 ctccgctgcc caccgcagac tgaggcagcg ctgagtcgcc ggcgccgcag cgcagatggt
      121 cgcgcccgtg cccccctatc tcgcgcctcg cgtggtgcgg tccggctggg ccggcggcgg
      181 cgcggacgcg accaaggtgg ccgggaaggg gagtttgcgg gggaccggcg agtgacgtca
      241 gcgcgccttc agtgctgagg cggcggtggc gcgcgccgcc aggcgggggc gaaggcactg
      301 tccgcggtgc tgaagctggc agtgcgcacg cgcctcgccg catcctgttt cccctccccc
      361 tctctgatag gggatgcgca atttggggaa tgggggttgg gtgcttgtcc agtgggtcgg
      421 ggtcggtcgt caggtaggca cccccacccc gcctcatcct ggtcctaaaa cccacttgca
      481 ctcatacgca gggccctctg cagggccgca ggaaccccta gtgatggagt tggccactcc
      541 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
      601 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
      661 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
      721 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      781 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
      841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
      961 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1021 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     1081 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     1141 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     1201 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     1261 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1321 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     1381 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1441 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1501 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1561 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1681 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1741 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1801 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1861 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1921 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1981 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2101 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2161 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2221 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2401 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2461 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2521 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3181 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3241 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
     3301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
     3361 catcactagg ggttcctgcg gccactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     3421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttctagtca
     3481 cttacttatc gatgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg
     3541 cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga
     3601 ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag
     3661 gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc
     3721 aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca
     3781 atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa
     3841 ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc
     3901 cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc
     3961 atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa
     4021 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
     4081 ttatcgatag aattctcg
//
