LOCUS       Exported                9678 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP 
            translatome from specific cell types defined by GAL4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    pUAST-attB-3xHA-V5-RpL10Ab
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Thomas A, Lee PJ, Dalton JE, Nomie KJ, Stoica L, Costa-Mattioli M, 
            Chang P, Nuzhdin S, Arbeitman MN, Dierick HA
  TITLE     A versatile method for cell-specific profiling of translated mRNAs 
            in Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 
            2012 Jul 6.
  PUBMED    22792260
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(10..28)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(480..551,953..1226,1301..1955,2017..2332,2536..2667,
                     2738..3352)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     complement(3813..3832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3932..3954
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3992..4010)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4078..4182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4183..5043
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4401..4420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5214..5802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5703..5722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6037..6622
                     /label=P element 5' end
     misc_feature    7478..7710
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    7772..7866
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7885..8123
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8070..8091
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             8162..8242
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             8252..8293
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8297..8306
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     intron          9040..9105
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9235..9255
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9527..9661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9564..9583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9618..9637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagtatgta tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt
       61 ttttttttta ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga
      121 tccaagggta tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca
      181 ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc
      241 cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc
      301 ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt
      361 aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg
      421 attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa
      481 tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc
      541 atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac
      601 tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac
      661 cgcaattgca caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat
      721 ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact
      781 atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa
      841 aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg
      901 ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag
      961 cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac
     1021 gctccggcca cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa
     1081 cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg
     1141 atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc
     1201 gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg
     1261 aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg
     1321 ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc
     1381 cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat
     1441 ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac
     1501 ctctttatcg gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg
     1561 ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt
     1621 tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc
     1681 ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg
     1741 atctgcgatg agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg
     1801 ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc
     1861 gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg
     1921 ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg
     1981 tatctagtat tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa
     2041 ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga
     2101 gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga
     2161 tatggagcag ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg
     2221 gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct
     2281 gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg
     2341 ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt
     2401 agatagaatt gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat
     2461 agttttgcag aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa
     2521 cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc
     2581 acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc
     2641 tttcaaaacg tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa
     2701 taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt
     2761 ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac
     2821 gattgccgaa ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc
     2881 gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct
     2941 ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc
     3001 gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt
     3061 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
     3121 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
     3181 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
     3241 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt
     3301 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     3361 tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta
     3421 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     3481 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     3541 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     3601 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     3661 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     3721 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggtgc
     3781 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3841 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3961 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4021 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctt cttcttgaac
     5821 tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc
     5881 aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca
     5941 tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc
     6001 gccgccgatc tgccgctgga ctacgtgggt ctggcccatg atgaaataac ataaggtggt
     6061 cccgtcgata gccgaagctt accgaagtat acacttaaat tcagtgcacg tttgcttgtt
     6121 gagaggaaag gttgtgtgcg gacgaatttt tttttgaaaa cattaaccct tacgtggaat
     6181 aaaaaaaaat gaaatattgc aaattttgct gcaaagctgt gactggagta aaattaattc
     6241 acgtgccgaa gtgtgctatt aagagaaaat tgtgggagca gagccttggg tgcagccttg
     6301 gtgaaaactc ccaaatttgt gatacccact ttaatgattc gcagtggaag gctgcacctg
     6361 caaaaggtca gacatttaaa aggaggcgac tcaacgcaga tgccgtacct agtaaagtga
     6421 tagagcctga accagaaaag ataaaagaag gctataccag tgggagtaca caaacagagt
     6481 aagtttgaat agtaaaaaaa atcatttatg taaacaataa cgtgactgtg cgttaggtcc
     6541 tgttcattgt ttaatgaaaa taagagcttg agggaaaaaa ttcgtacttt ggagtacgaa
     6601 atgcgtcgtt tagagcagca gccgaattaa ttctagttcc agtgaaatcc aagcattttc
     6661 taaattaaat gtattcttat tattatagtt gttatttttg atatatataa acaacactat
     6721 tatgcccacc atttttttga gatgcatcta cacaaggaac aaacactgga tgtcactttc
     6781 agttcaaatt gtaacgctaa tcactccgaa caggtcacaa aaaattacct taaaaagtca
     6841 taatattaaa ttagaataaa tatagctgtg agggaaatat atacaaatat attggagcaa
     6901 ataaattgta catacaaata tttattacta atttctattg agacgaaatg aaccactcgg
     6961 aaccatttga gcgaaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac
     7021 aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt
     7081 ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa
     7141 tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt
     7201 aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat
     7261 acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt
     7321 ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aaacaagcag
     7381 ttatttcgga tatatgtcgg ctactccttg cgtcgggccc gaagtcttag agccagatat
     7441 gcgagcaccc ggaagctcac gatgagaatg gccagaccat gatgaaataa cataaggtgg
     7501 tcccgtcggc aagagacatc cacttaacgt atgcttgcaa taagtgcgag tgaaaggaat
     7561 agtattctga gtgtcgtatt gagtctgagt gagacagcga tatgattgtt gattaaccct
     7621 tagcatgtcc gtggggtttg aattaactca taatattaat tagacgaaat tatttttaaa
     7681 gttttatttt taataatttg cgagtacgca aagcttggct gcatccaacg cgttgggagc
     7741 tctccggatc caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     7801 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     7861 tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt ctacggagcg
     7921 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
     7981 aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta
     8041 aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta
     8101 ttgaatacaa gaagagaact ctgaataggg aattgggaat tcgttaacag atctcaaaat
     8161 gtacccatac gacgtcccag actacgctta cccatacgac gtcccagact acgcttaccc
     8221 atacgacgtc ccagactacg ctgccggtac cggtaagcct atccctaacc ctctcctcgg
     8281 tctcgattct acggcggccg ccatggcgtc gaaggtttcg cgtgatacgc tgtatgaggg
     8341 cgtcaatgga ctcctggagg cttcggcgaa gaagaagcgc ggcttcctcg agacggtgga
     8401 actgcagatc ggcctgaaga actacgatcc ccagaaggac aagcgtttct ccggcaccgt
     8461 caagttgaag cacattcctc gtcccaagat gaaggtgtgc atccttggcg atcagcagca
     8521 ttgcgacgaa gccaaggcca acaacgtcga cttcatggat gccgaggctc tgaagaagct
     8581 gaacaagaac aagaagctgg tgaagaagct ggccaagtcc tatgatgcct tcctggcctc
     8641 tgagtcgctg atcaagcaga ttcctcgttt gcttggccct ggcttgaaca aggccggcaa
     8701 gttccctgcc ctgctgtcgc atcaggagtc tatgattggc aagatcgagg aggttaagtc
     8761 gaccatcaag ttccagatga agaaggtgct ctgcctgtcc gtcgccgtcg gccacgttgg
     8821 catgaagtcc gacgagctgg cccaaaacgt caacttgtcg atcaacttct tggtgtcgct
     8881 gttgaagaag aactggcaga atgtgcgctc cctgcacgtc aagtcctcca tgggaccgcc
     8941 ccagcgtctt tactagtcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca
     9001 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     9061 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     9121 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     9181 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     9241 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     9301 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     9361 gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc cttgactaga
     9421 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9481 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9661 ccactagaag gcctaatt
//
