LOCUS Exported 10224 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Entry plasmid for guide cloning. ACCESSION . VERSION . KEYWORDS CROPseq-puro-v2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10224) AUTHORS Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity AJ, Zhang F, Blainey PC TITLE Optical Pooled Screens in Human Cells. JOURNAL Cell. 2019 Oct 17;179(3):787-799.e17. doi: 10.1016/j.cell.2019.09.016. PUBMED 31626775 REFERENCE 2 (bases 1 to 10224) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10224 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 50..290 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 50..70 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 221..240 /label=LKO.1 5' /note="Human U6 promoter, forward primer" LTR 2318..2498 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 2576..2697 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2613..2632) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2667..2686 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 2737..2872 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2799..2818 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(2892..2911) /label=T7 /note="T7 promoter, forward primer" promoter complement(2893..2911) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2921..2938) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2921..2937) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2930..2952) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3079..3534 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3166..3185) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3376..3397 /label=F1ori-F /note="F1 origin, forward primer" promoter 3560..3664 /gene="bla" /label=AmpR promoter CDS 3665..4525 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3883..3902) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4696..5284 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5185..5204 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5438..5455 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5572..5593 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5608..5638 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5646..5662 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5651..5673 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5670..5686 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5670..5686 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 5705..5725 /label=T3 /note="T3 promoter, forward primer" promoter 5707..5725 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 5753..5979 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 5980..6160 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 6207..6332 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6825..7058 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7243..7287 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 7468..7585 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7724..8902 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 7955..8893 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 8850..8870 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 8915..9514 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8915..8934) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9411..9431 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9530..10118 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9583..9603) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10001..10012) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt 61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat 121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt 181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt 241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg 301 agacggttgt aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt 361 ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag aatttttatg 421 ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac 481 tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag 541 ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa 601 ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg 661 attttgaaaa tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac 721 aagctttcat gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat 781 gtgatccagc tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt 841 cttttaatgc accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt 901 cagaaacttc gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat 961 ttgttattcc tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat 1021 taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag 1081 ctttcgtagc atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt 1141 ctcctatata ttcattttta attttaattc tttaataatt tcgtctactt taactttagc 1201 gttttgaaca gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact 1261 tgggcgaaca gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg 1321 catattatac attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg 1381 agttaagggt gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc 1441 tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc 1501 ttctaaatag tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat 1561 taatatagaa gcttgtattc catctttatc tctagctgag tcatcaatta catatccata 1621 actttcttca taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct 1681 acccattcat ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc 1741 gattctatca cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat 1801 gctatttaag cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt 1861 tccatctaat cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc 1921 tgggtcattc ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt 1981 tcatgcagga tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa 2041 tgcatgctct tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa 2101 tttagttcct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg 2161 ctcttttttg tacgtctctg tttcagagct atgctggaaa cagcatagca agttgaaata 2221 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc 2281 gtaactagat cttgagacac tgctttttgc ttgtactggg tctctctggt tagaccagat 2341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 2401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 2461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca 2521 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 2581 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 2641 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 2701 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 2761 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 2821 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg 2881 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac 2941 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 3001 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 3061 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3121 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3181 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 3241 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 3301 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 3361 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 3421 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 3481 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg 3541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 3601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 3661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 3721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 3781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 3841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 3901 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 3961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 4021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 4081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 4141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 4201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 4261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 4321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 4381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 4441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 4501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 4561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 4621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 4681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 4741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 4801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 4861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 4921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 4981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 5041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 5101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 5161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 5221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 5281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 5341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 5401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 5461 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 5521 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 5581 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 5641 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 5701 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct 5761 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 5821 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 5881 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 5941 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 6001 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 6061 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 6121 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 6181 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 6241 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 6301 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 6361 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 6421 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 6481 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 6541 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 6601 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 6661 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga 6721 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag 6781 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt 6841 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag 6901 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag 6961 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc 7021 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga 7081 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa 7141 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc 7201 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta 7261 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg 7321 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct 7381 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt 7441 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag 7501 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa 7561 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg 7621 gcgccggctc gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct 7681 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca 7741 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 7801 atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 7861 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 7921 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 7981 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 8041 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 8101 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 8161 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 8221 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 8281 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 8341 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 8401 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 8461 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 8521 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 8581 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 8641 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 8701 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 8761 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 8821 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 8881 tcttccattt caggtgtcgt gacgtacggc caccatgacc gagtacaagc ccacggtgcg 8941 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 9001 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 9061 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 9121 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 9181 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 9241 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 9301 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 9361 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 9421 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 9481 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct 9541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 9601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 9661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 9721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 9781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 9841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 9901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 9961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 10021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 10081 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact 10141 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 10201 attcactccc aacgaagaca agat //