LOCUS       Exported               15383 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses dCas9-VPR driven by the Ubiquitin-63E promoter.
ACCESSION   .
VERSION     .
KEYWORDS    OA-986B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15383)
  AUTHORS   .
  TITLE     Engineering reproductive isolation in D. melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15383
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    194..263
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     regulatory      2340..2349
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2346..6449
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6462..6482
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6507..6656
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6681..6701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7131..7487
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7506..8075
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             9319..10038
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9364..9385)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9625..9644)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9972..9993
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10160..10281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10197..10216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10251..10270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   10946..10980
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11161..11177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11161..11177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11174..11196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11185..11201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11209..11239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11254..11275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11392..11409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11563..12151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11643..11662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12322..13182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12945..12964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13183..13287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13355..13373
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13411..13433)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13533..13552
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13746..13768
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13760..13777
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13761..13777
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   14481..14543
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
       61 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
      121 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggtcgacga
      181 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
      241 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
      301 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
      361 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
      421 tcagcgggtt ctcgacggtc acggcgggca tgtcgacgcg gccgccgcgc agatcgccga
      481 tgggggtggc gccggagggc accttcacct tgccgtacgg cttgctgttc ttcgcgttca
      541 aaatctccag ctccattttg ctttcggtgc gcttgcaatc agtactgtcc aaaatcgaaa
      601 atcgccgaac cgtagtgtga ccgtgcgggg ctctgcgaaa ataaactttt ttaggtatat
      661 ggccacacac ggggaaagca cagtggatta tatgttttaa tattataata tgcaggtttt
      721 cattacttat ccagatgtaa gcccacttaa agcgatttaa caattatttg ccgaaagagt
      781 ataaacaaat ttcacttaaa aatggattaa gaaaagcttg tgtaagatta tgcgcagcgt
      841 tgccagatag ctccatttaa aacacttcaa aaacaataag ttttgaaaat atatacataa
      901 atagcagtcg ttgccgcaac gctcaacaca tcacactttt aaaacaccct ttacctacac
      961 agaattactt tttaaatttc cagtcaagct gcgagtttca aaattatagc cggtagagaa
     1021 gacagtgcta tttcaaaagc aaactaacaa gggtcttaaa ttccaaaaca ccaatcctaa
     1081 caagccttgg acttttgtaa gtttagatca aaggtggcat tgcattcaat gtcatggtaa
     1141 gaagtaggtc gtctaggtag aaatcctcat tcagccggtc aagtcagtac gagaaaggtc
     1201 tcaatttgaa attgtcttaa aaatatttta ttgttttgta ctgtggtgag tttaaacgaa
     1261 aaacacaaaa aaaaagtgat acacagaaat cataaaaaat tttaatacaa ggtattcgta
     1321 cgtatcaaaa acatttcggc acaatttttt ttctctgtac taaagtgtta cgaacactac
     1381 ggtatttttt agtgattttc aacggacacc gaaggtatat aaacagcgtt cgcgaacggt
     1441 cgccttcaaa accaattgac atttgcagca gcaagtacaa gtagaaagta aagcgcaatc
     1501 agcgaaaaat ttatacttaa ttgttggtga ttaaagtaca attaaaagaa cattctcgaa
     1561 agtcacaaga aacgtaagtt tttaactcgc tgttaccaat tagtaataag agcaacaaga
     1621 cgttgagtaa tttcaagaaa aactgcattt caaggtcttt gttcggccat ttttttttta
     1681 ttcaacgctc tacgtaatta caaaataaga aattggcagc cacgcatctt gttttcccaa
     1741 tgaattggca tcaaaacgca aacaaatcta taaataaaac ttgcgtgttg attttcgcca
     1801 agatttattg gcaaattgtg aaattcgcag tgacgcattt gaaaattcga gaaatcacga
     1861 acgcactcga gcatttgtgt gcatgttatt agttagttgt ttagttaatt gaagtatttt
     1921 accaacgaaa tccacttatt tttagctgaa atagagtagg ttgcttaaac aaagccacgt
     1981 ctgaaaattt cttattgctt gtagttgtga cgtcaccata tacacacaaa ataatgtgta
     2041 tgcatgcatt tcagctgtgt atatatacat gcacacactc gcaacacgaa aacgatgacg
     2101 aagcaacgga acaaaggttt ctcaactacc ctttgttccc tgtttcttcg ctttcctttg
     2161 ttccaatatt cgtagagggt taataggggt ttctcaacaa agttggcgtc gataaataag
     2221 tttcccattt ttattcccca gccaggaagt tagtttcaat agttttgtaa tttcaacgaa
     2281 actcatttga tttcgtacta attttccaca tctctatttt gacccgcaga gtttaaactg
     2341 ccaccatgga caagaagtac tccattgggc tcgctatcgg cacaaacagc gtcggctggg
     2401 ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
     2461 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggagacgg
     2521 ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
     2581 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
     2641 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
     2701 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
     2761 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
     2821 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
     2881 gcgatgtcga taaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
     2941 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
     3001 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
     3061 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
     3121 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
     3181 tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg
     3241 ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg
     3301 ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg
     3361 tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct
     3421 acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca
     3481 tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt
     3541 tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac
     3601 tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa
     3661 agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa
     3721 attccagatt cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg
     3781 aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg
     3841 ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca
     3901 cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat
     3961 tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag
     4021 ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg
     4081 aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga
     4141 aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca
     4201 ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt
     4261 acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat
     4321 gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa
     4381 tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg
     4441 atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca
     4501 gtcttcacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc
     4561 agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata
     4621 tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg
     4681 aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac
     4741 acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg
     4801 gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg
     4861 ctgctatcgt gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa
     4921 gatccgataa agctagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa
     4981 tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata
     5041 atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa
     5101 ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca
     5161 tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga
     5221 agtctaagct ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca
     5281 acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca
     5341 aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta
     5401 ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt
     5461 acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga
     5521 agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg
     5581 atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg
     5641 aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc
     5701 tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag
     5761 tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa
     5821 gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca
     5881 tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc
     5941 ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg
     6001 agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg
     6061 ccagccacta tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg
     6121 tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa
     6181 gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg
     6241 ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg
     6301 gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta
     6361 caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa
     6421 gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg
     6481 tggaggccag cggttccgga cgggctgacg cattggacga ttttgatctg gatatgctgg
     6541 gaagtgacgc cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact
     6601 ttgacctcga catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta
     6661 actctagaag ttccggatct ccgaaaaaga aacgcaaagt tggtagccag tacctgcccg
     6721 acaccgacga ccggcaccgg atcgaggaaa agcggaagcg gacctacgag acattcaaga
     6781 gcatcatgaa gaagtccccc ttcagcggcc ccaccgaccc tagacctcca cctagaagaa
     6841 tcgccgtgcc cagcagatcc agcgccagcg tgccaaaacc tgccccccag ccttacccct
     6901 tcaccagcag cctgagcacc atcaactacg acgagttccc taccatggtg ttccccagcg
     6961 gccagatctc tcaggcctct gctctggctc cagcccctcc tcaggtgctg cctcaggctc
     7021 ctgctcctgc accagctcca gccatggtgt ctgcactggc tcaggcacca gcacccgtgc
     7081 ctgtgctggc tcctggacct ccacaggctg tggctccacc agcccctaaa cctacacagg
     7141 ccggcgaggg cacactgtct gaagctctgc tgcagctgca gttcgacgac gaggatctgg
     7201 gagccctgct gggaaacagc accgatcctg ccgtgttcac cgacctggcc agcgtggaca
     7261 acagcgagtt ccagcagctg ctgaaccagg gcatccctgt ggcccctcac accaccgagc
     7321 ccatgctgat ggaatacccc gaggccatca cccggctcgt gacaggcgct cagaggcctc
     7381 ctgatccagc tcctgcccct ctgggagcac caggcctgcc taatggactg ctgtctggcg
     7441 acgaggactt cagctctatc gccgacatgg acttctccgc actgctgggt agcggatcgg
     7501 gatctcggga ttccagggaa gggatgtttt tgccgaagcc tgaggccggc tccgctatta
     7561 gtgacgtgtt tgagggccgc gaggtgtgcc agccaaaacg aatccggcca tttcatcctc
     7621 caggaagtcc atgggccaac cgcccactcc ccgccagcct cgcaccaaca ccaaccggtc
     7681 cagtacatga gccagtcggg tcactgaccc cggcaccagt ccctcagcca ctggatccag
     7741 cgcccgcagt gactcccgag gccagtcacc tgttggagga tcccgatgaa gagacgagcc
     7801 aggctgtcaa agcccttcgg gagatggccg atactgtgat tccccagaag gaagaggctg
     7861 caatctgtgg ccaaatggac ctttcccatc cgcccccaag gggccatctg gatgagctga
     7921 caaccacact tgagtccatg accgaggatc tgaacctgga ctcacccctg accccggaat
     7981 tgaacgagat tctggatacc ttcctgaacg acgagtgcct cttgcatgcc atgcatatca
     8041 gcacaggact gtccatcttc gacacatctc tgttttgatt aattaactag aatgaatcgt
     8101 ttttaaaata acaaatcaat tgttttataa tattcgtacg attctttgat tatgtaataa
     8161 aatgtgatca ttaggaagat tacgaaaaat ataaaaaata tgagttctgt gtgtataaca
     8221 aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac ccatgattat ttgattaaaa
     8281 ttgttgtttt ctttgttcat agacaatagt gtgttttgcc taaacgtgta ctgcataaac
     8341 tccatgcgag tgtatagcga gctagtggct aacgcttgcc ccaccaaagt agattcgtca
     8401 aaatcctcaa tttcatcacc ctcctccaag tttaacattt ggccgtcgga attaacttct
     8461 aaagatgcca cataatctaa taaatgaaat agagattcaa acgtggcgtc atcgtccgtt
     8521 tcgaccattt ccgaaaagaa ctcgggcata aactctatga tttctctgga cgtggtgttg
     8581 tcgaaactct caaagtacgc agtcaggaac gtgcgcgaca tgtcgtcggg aaactcgcgc
     8641 ggaaacatgt tgttgtaacc gaacgggtcc catagcgcca aaaccaaatc tgccagcgtc
     8701 aatagaatga gcacgatgcc gacaatggag ctggcttgga tagcgattcg agttaacggc
     8761 cggggcgcgc ccatactcgg tggcctcccc accaccaact tttttgcact gcaaaaaaac
     8821 acgcttttgc acgcgggccc atacatagta caaactctac gtttcgtaga ctattttaca
     8881 taaatagtct acaccgttgt atacgctcca aatacactac cacacattga acctttttgc
     8941 agtgcaaaaa agtacgtgtc ggcagtcacg taggccggcc ttatcgggtc gcgtcctgtc
     9001 acgtacgaat cacattatcg gaccggacga gtgttgtctt atcgtgacag gacgccagct
     9061 tcctgtgttg ctaaccgcag ccggacgcaa ctccttatcg gaacaggacg cgcctccata
     9121 tcagccgcgc gttatctcat gcgcgtgacc ggacacgagg cgcccgtccc gcttatcgcg
     9181 cctataaata cagcccgcaa cgatctggta aacacagttg aacagcatct gttacagcga
     9241 cacaacatga gccggtccaa caacgccaac gcgcccacgc catccaaccg ccgccgcaac
     9301 ctgtctctgg tgctcgagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     9361 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     9421 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     9481 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
     9541 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     9601 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     9661 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     9721 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc
     9781 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     9841 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9901 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     9961 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
    10021 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
    10081 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10141 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10261 caaactcatc aatgtatctt aaagcttggc cggccgatct cggatctgac aatgttcagt
    10321 gcagagactc ggctacgcct cgtggacttt gaagttgacc aacaatgttt attcttacct
    10381 ctaatagtcc tctgtggcaa ggtcaagatt ctgttagaag ccaatgaaga acctggttgt
    10441 tcaataacat tttgttcgtc taatatttca ctaccgcttg acgttggctg cacttcatgt
    10501 acctcatcta taaacgcttc ttctgtatcg ctctggacgt catcttcact tacgtgatct
    10561 gatatttcac tgtcagaatc ctcaccaaca agctcgtcat cgctttgcag aagagcagag
    10621 aggatatgct catcgtctaa agaactaccc attttattat atattagtca cgatatctat
    10681 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa
    10741 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc
    10801 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg
    10861 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga
    10921 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg
    10981 ttaaaaaaga tttgcgcttt actcgaccta aactttaaac acgtcataga atcttcgttt
    11041 gacaaaaacc acattgtggc caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc
    11101 aagtcgcgcg agcgtcgact ctagaggatc cccgggtacc gagctcgaat tcgtaatcat
    11161 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    11221 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    11281 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    11341 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    11401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    11461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    11521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    12001 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    12061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    12121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    12181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    12241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    12301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    12361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    12421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    13021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    13081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    13141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    13201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    13261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    13321 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    13381 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    13441 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    13501 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    13561 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    13621 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    13681 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    13741 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctttgtt taaaaatata
    13801 acaaaattgt gatcccacaa aatgaagtgg ggcaaaatca aataattaat agtgtccgta
    13861 aacttgttgg tcttcaactt tttgaggaac acgttggacg gcaaatccgt gactataaca
    13921 caagttgatt taataatttt agccaacacg tcgggctgcg tgttttttgc cgacgcgtct
    13981 gtgtacacgt tgattaactg gtcgattaaa ctgttgaaat aatttaattt ttggttcttc
    14041 tttaaatctg tgatgaaatt ttttaaaata actttaaatt cttcattggt aaaaaatgcc
    14101 acgttttgca acttgtgagg gtctaatatg aggtcaaact cagtaggagt tttatccaaa
    14161 aaagaaaaca tgattacgtc tgtacacgaa cgcgtattaa cgcagagtgc aaagtataag
    14221 agggttaaaa aatatatttt acgcaccata tacgcatcgg gttgatatcg ttaatatgga
    14281 tcaatttgaa cagttgatta acgtgtctct gctcaagtct ttgatcaaaa cgcaaatcga
    14341 cgaaaatgtg tcggacaata tcaagtcgat gagcgaaaaa ctaaaaaggc tagaatacga
    14401 caatctcaca gacagcgttg agatatacgg tattcacgac agcaggctga ataataaaaa
    14461 aattagaaac tattatttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata
    14521 atcatgcgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa
    14581 tttgaataga tattaagttt tattatattt acacttacat actaataata aattcaacaa
    14641 acaatttatt tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat
    14701 aaagtaacaa aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta
    14761 aaaacagtca tgttgtatta taaaataagt aattagctta acttatacat aatagaaaca
    14821 aattatactt attagtcagt cagaaacaac tttggcacat atcaatatta tgctctcgac
    14881 aaataacttt tttgcatttt ttgcacgatg catttgcctt tcgccttatt ttagaggggc
    14941 agtaagtaca gtaagtacgt tttttcatta ctggctcttc agtactgtca tctgatgtac
    15001 caggcacttc atttggcaaa atattagaga tattatcgcg caaatatctc ttcaaagtag
    15061 gagcttctaa acgcttacgc ataaacgatg acgtcaggct catgtaaagg tttctcataa
    15121 attttttgcg actttgaacc ttttctccct tgctactgac attatggctg tatataataa
    15181 aagaatttat gcaggcaatg tttatcattc cgtacaataa tgccataggc cacctattcg
    15241 tcttcctact gcaggtcatc acagaacaca tttggtctag cgtgtccact ccgcctttag
    15301 tttgattata atacataacc atttgcggtt taccggtact ttcgttgata gaagcatcct
    15361 catcacaaga tgataataag tat
//
