LOCUS       Exported               14525 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter.
ACCESSION   .
VERSION     .
KEYWORDS    OA-986D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   .
  TITLE     Engineering reproductive isolation in D. melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1904..1951
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1952..6052
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6107..6127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6152..6301
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6323..6376
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6377..6775
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             8016..8735
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8061..8082)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8322..8341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8669..8690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8857..8978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8894..8913)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8948..8967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9643..9677
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9858..9874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9858..9874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9871..9893)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9882..9898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9906..9936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9951..9972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10089..10106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10260..10848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10340..10359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11019..11879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11642..11661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11880..11984)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12052..12070
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12108..12130)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12230..12249
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12443..12465
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12457..12474
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12458..12474
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   13178..13240
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    14274..14343
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 gggcatgtcg acgcggccgc cgcgcagatc gccgatgggg gtggcgccgg agggcacctt
       61 caccttgccg tacggcttgc tgttcttcgc gttcaaaatc tccagctcca ttttgctttc
      121 ggtgcgcttg caatcagtac tgtccaaaat cgaaaatcgc cgaaccgtag tgtgaccgtg
      181 cggggctctg cgaaaataaa cttttttagg tatatggcca cacacgggga aagcacagtg
      241 gattatatgt tttaatatta taatatgcag gttttcatta cttatccaga tgtaagccca
      301 cttaaagcga tttaacaatt atttgccgaa agagtataaa caaatttcac ttaaaaatgg
      361 attaagaaaa gcttgtgtaa gattatgcgc agcgttgcca gatagctcca tttaaaacac
      421 ttcaaaaaca ataagttttg aaaatatata cataaatagc agtcgttgcc gcaacgctca
      481 acacatcaca cttttaaaac accctttacc tacacagaat tactttttaa atttccagtc
      541 aagctgcgag tttcaaaatt atagccggta gagaagacag tgctatttca aaagcaaact
      601 aacaagggtc ttaaattcca aaacaccaat cctaacaagc cttggacttt tgtaagttta
      661 gatcaaaggt ggcattgcat tcaatgtcat ggtaagaagt aggtcgtcta ggtagaaatc
      721 ctcattcagc cggtcaagtc agtacgagaa aggtctcaat ttgaaattgt cttaaaaata
      781 ttttattgtt ttgtactgtg gtgagtttaa acgaaaaaca caaaaaaaaa gtgatacaca
      841 gaaatcataa aaaattttaa tacaaggtat tcgtacgtat caaaaacatt tcggcacaat
      901 tttttttctc tgtactaaag tgttacgaac actacggtat tttttagtga ttttcaacgg
      961 acaccgaagg tatataaaca gcgttcgcga acggtcgcct tcaaaaccaa ttgacatttg
     1021 cagcagcaag tacaagtaga aagtaaagcg caatcagcga aaaatttata cttaattgtt
     1081 ggtgattaaa gtacaattaa aagaacattc tcgaaagtca caagaaacgt aagtttttaa
     1141 ctcgctgtta ccaattagta ataagagcaa caagacgttg agtaatttca agaaaaactg
     1201 catttcaagg tctttgttcg gccatttttt ttttattcaa cgctctacgt aattacaaaa
     1261 taagaaattg gcagccacgc atcttgtttt cccaatgaat tggcatcaaa acgcaaacaa
     1321 atctataaat aaaacttgcg tgttgatttt cgccaagatt tattggcaaa ttgtgaaatt
     1381 cgcagtgacg catttgaaaa ttcgagaaat cacgaacgca ctcgagcatt tgtgtgcatg
     1441 ttattagtta gttgtttagt taattgaagt attttaccaa cgaaatccac ttatttttag
     1501 ctgaaataga gtaggttgct taaacaaagc cacgtctgaa aatttcttat tgcttgtagt
     1561 tgtgacgtca ccatatacac acaaaataat gtgtatgcat gcatttcagc tgtgtatata
     1621 tacatgcaca cactcgcaac acgaaaacga tgacgaagca acggaacaaa ggtttctcaa
     1681 ctaccctttg ttccctgttt cttcgctttc ctttgttcca atattcgtag agggttaata
     1741 ggggtttctc aacaaagttg gcgtcgataa ataagtttcc catttttatt ccccagccag
     1801 gaagttagtt tcaatagttt tgtaatttca acgaaactca tttgatttcg tactaatttt
     1861 ccacatctct attttgaccc gcagagttta aactgccacc atgaaaaggc cggcggccac
     1921 gaaaaaggcc ggccaggcaa aaaagaaaaa ggacaagaag tacagcatcg gcctggccat
     1981 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
     2041 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
     2101 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
     2161 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     2221 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     2281 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     2341 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     2401 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     2461 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     2521 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     2581 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     2641 cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc tgacccccaa
     2701 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     2761 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     2821 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     2881 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     2941 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     3001 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     3061 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     3121 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     3181 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     3241 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     3301 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     3361 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     3421 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     3481 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     3541 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     3601 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     3661 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     3721 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     3781 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     3841 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     3901 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     3961 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     4021 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     4081 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     4141 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     4201 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     4261 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     4321 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     4381 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     4441 ccggctgtcc gactacgatg tggaccacat cgtgcctcag agctttctga aggacgactc
     4501 catcgacaac aaggtgctga ccagaagcga caaggcccgg ggcaagagcg acaacgtgcc
     4561 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     4621 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     4681 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     4741 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     4801 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     4861 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     4921 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     4981 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     5041 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     5101 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     5161 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     5221 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     5281 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     5341 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     5401 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     5461 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     5521 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     5581 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     5641 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     5701 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     5761 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     5821 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     5881 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     5941 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     6001 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acagcgctgg
     6061 aggaggtgga agcggaggag gaggaagcgg aggaggaggt agcggaccta agaaaaagag
     6121 gaaggtggcg gccgctggat ccggacgggc tgacgcattg gacgattttg atctggatat
     6181 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga
     6241 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct
     6301 gattaactgt acaggcagtg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga
     6361 ggagaatcct ggcccaatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     6421 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     6481 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     6541 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     6601 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     6661 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     6721 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaattaat
     6781 taactagaat gaatcgtttt taaaataaca aatcaattgt tttataatat tcgtacgatt
     6841 ctttgattat gtaataaaat gtgatcatta ggaagattac gaaaaatata aaaaatatga
     6901 gttctgtgtg tataacaaat gctgtaaacg ccacaattgt gtttgttgca aataaaccca
     6961 tgattatttg attaaaattg ttgttttctt tgttcataga caatagtgtg ttttgcctaa
     7021 acgtgtactg cataaactcc atgcgagtgt atagcgagct agtggctaac gcttgcccca
     7081 ccaaagtaga ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt aacatttggc
     7141 cgtcggaatt aacttctaaa gatgccacat aatctaataa atgaaataga gattcaaacg
     7201 tggcgtcatc gtccgtttcg accatttccg aaaagaactc gggcataaac tctatgattt
     7261 ctctggacgt ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg cgcgacatgt
     7321 cgtcgggaaa ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa
     7381 ccaaatctgc cagcgtcaat agaatgagca cgatgccgac aatggagctg gcttggatag
     7441 cgattcgagt taacggccgg ggcgcgccca tactcggtgg cctccccacc accaactttt
     7501 ttgcactgca aaaaaacacg cttttgcacg cgggcccata catagtacaa actctacgtt
     7561 tcgtagacta ttttacataa atagtctaca ccgttgtata cgctccaaat acactaccac
     7621 acattgaacc tttttgcagt gcaaaaaagt acgtgtcggc agtcacgtag gccggcctta
     7681 tcgggtcgcg tcctgtcacg tacgaatcac attatcggac cggacgagtg ttgtcttatc
     7741 gtgacaggac gccagcttcc tgtgttgcta accgcagccg gacgcaactc cttatcggaa
     7801 caggacgcgc ctccatatca gccgcgcgtt atctcatgcg cgtgaccgga cacgaggcgc
     7861 ccgtcccgct tatcgcgcct ataaatacag cccgcaacga tctggtaaac acagttgaac
     7921 agcatctgtt acagcgacac aacatgagcc ggtccaacaa cgccaacgcg cccacgccat
     7981 ccaaccgccg ccgcaacctg tctctggtgc tcgagatggt gagcaagggc gaggagctgt
     8041 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     8101 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     8161 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg
     8221 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     8281 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     8341 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     8401 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc
     8461 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     8521 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     8581 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     8641 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     8701 ggatcactct cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     8761 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     8821 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     8881 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8941 tctagttgtg gtttgtccaa actcatcaat gtatcttaaa gcttggccgg ccgatctcgg
     9001 atctgacaat gttcagtgca gagactcggc tacgcctcgt ggactttgaa gttgaccaac
     9061 aatgtttatt cttacctcta atagtcctct gtggcaaggt caagattctg ttagaagcca
     9121 atgaagaacc tggttgttca ataacatttt gttcgtctaa tatttcacta ccgcttgacg
     9181 ttggctgcac ttcatgtacc tcatctataa acgcttcttc tgtatcgctc tggacgtcat
     9241 cttcacttac gtgatctgat atttcactgt cagaatcctc accaacaagc tcgtcatcgc
     9301 tttgcagaag agcagagagg atatgctcat cgtctaaaga actacccatt ttattatata
     9361 ttagtcacga tatctataac aagaaaatat atatataata agttatcacg taagtagaac
     9421 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc
     9481 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg
     9541 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg
     9601 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc
     9661 agactatctt tctagggtta aaaaagattt gcgctttact cgacctaaac tttaaacacg
     9721 tcatagaatc ttcgtttgac aaaaaccaca ttgtggccaa gctgtgtgac gcgacgcgcg
     9781 ctaaagaatg gcaaaccaag tcgcgcgagc gtcgactcta gaggatcccc gggtaccgag
     9841 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9901 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9961 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    10141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10681 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10921 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10981 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11041 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11101 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11161 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11221 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11281 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11341 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11401 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11461 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11521 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11581 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11641 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11701 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11761 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11821 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11881 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11941 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12001 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    12061 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    12121 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    12181 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    12241 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    12301 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    12361 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    12421 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    12481 ctttgtttaa aaatataaca aaattgtgat cccacaaaat gaagtggggc aaaatcaaat
    12541 aattaatagt gtccgtaaac ttgttggtct tcaacttttt gaggaacacg ttggacggca
    12601 aatccgtgac tataacacaa gttgatttaa taattttagc caacacgtcg ggctgcgtgt
    12661 tttttgccga cgcgtctgtg tacacgttga ttaactggtc gattaaactg ttgaaataat
    12721 ttaatttttg gttcttcttt aaatctgtga tgaaattttt taaaataact ttaaattctt
    12781 cattggtaaa aaatgccacg ttttgcaact tgtgagggtc taatatgagg tcaaactcag
    12841 taggagtttt atccaaaaaa gaaaacatga ttacgtctgt acacgaacgc gtattaacgc
    12901 agagtgcaaa gtataagagg gttaaaaaat atattttacg caccatatac gcatcgggtt
    12961 gatatcgtta atatggatca atttgaacag ttgattaacg tgtctctgct caagtctttg
    13021 atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca agtcgatgag cgaaaaacta
    13081 aaaaggctag aatacgacaa tctcacagac agcgttgaga tatacggtat tcacgacagc
    13141 aggctgaata ataaaaaaat tagaaactat tatttaaccc tagaaagata atcatattgt
    13201 gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg tgttttatcg gtctgtatat
    13261 cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca cttacatact
    13321 aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa aaaacaaaaa
    13381 ctcaaaattt cttctataaa gtaacaaaac ttttaaacat tctctctttt acaaaaataa
    13441 acttattttg tactttaaaa acagtcatgt tgtattataa aataagtaat tagcttaact
    13501 tatacataat agaaacaaat tatacttatt agtcagtcag aaacaacttt ggcacatatc
    13561 aatattatgc tctcgacaaa taactttttt gcattttttg cacgatgcat ttgcctttcg
    13621 ccttatttta gaggggcagt aagtacagta agtacgtttt ttcattactg gctcttcagt
    13681 actgtcatct gatgtaccag gcacttcatt tggcaaaata ttagagatat tatcgcgcaa
    13741 atatctcttc aaagtaggag cttctaaacg cttacgcata aacgatgacg tcaggctcat
    13801 gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt tctcccttgc tactgacatt
    13861 atggctgtat ataataaaag aatttatgca ggcaatgttt atcattccgt acaataatgc
    13921 cataggccac ctattcgtct tcctactgca ggtcatcaca gaacacattt ggtctagcgt
    13981 gtccactccg cctttagttt gattataata cataaccatt tgcggtttac cggtactttc
    14041 gttgatagaa gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg
    14101 tttatatgag acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct
    14161 ggagcgactg tttttcagta cttccggtat ctcgcgtttg tttgatcgca cggttcccac
    14221 aatggttaat tcgagctcgc ccggggtcct aggtcgacga tgtaggtcac ggtctcgaag
    14281 ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc
    14341 atctggtcca tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg
    14401 cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga
    14461 cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc
    14521 acggc
//
