LOCUS Exported 7148 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION ATF4 expression vector. ACCESSION . VERSION . KEYWORDS RFP-ATF4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7148) AUTHORS Bagheri-Yarmand R, Sinha KM, Gururaj AE, Ahmed Z, Rizvi YQ, Huang SC, Ladbury JE, Bogler O, Williams MD, Cote GJ, Gagel RF TITLE A novel dual kinase function of the RET proto-oncogene negatively regulates activating transcription factor 4-mediated apoptosis. JOURNAL J Biol Chem. 2015 May 1;290(18):11749-61. doi: 10.1074/jbc.M114.619833. Epub 2015 Mar 20. PUBMED 25795775 REFERENCE 2 (bases 1 to 7148) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7148 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1738..1755 /codon_start=1 /product="N-myristoylation signal from Src kinase (Pellman et al., 1985; Kaplan et al., 1988)" /label=myr /translation="GSSKSK" CDS 2024..2701 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" primer_bind complement(2154..2172) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2422..2441 /label=mCherry-F /note="mCherry, forward primer" primer_bind 2616..2639 /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(2739..2756) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2745..2969 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3015..3443 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3102..3121) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3312..3333 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3452..3472) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3457..3786 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3637..3772 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3699..3718 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3853..4647 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3907..3926) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4517..4536 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4821..4942 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4858..4877) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4912..4931 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4991..5007) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4991..5007) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5004..5026) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5015..5031 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5039..5069) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5084..5105 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5222..5239) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5393..5981) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5473..5492) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6152..7012) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6775..6794 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7013..7117) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccatgacc gaaatgagct tcctgagcag 961 cgaggtgttg gtgggggact tgatgtcccc cttcgaccag tcgggtttgg gggctgaaga 1021 aagcctaggt ctcttagatg attacctgga ggtggccaag cacttcaaac ctcatgggtt 1081 ctccagcgac aaggctaagg cgggctcctc cgaatggctg gctgtggatg ggttggtcag 1141 tccctccaac aacagcaagg aggatgcctt ctccgggaca gattggatgt tggagaaaat 1201 ggatttgaag gagttcgact tggatgccct gttgggtata gatgacctgg aaaccatgcc 1261 agatgacctt ctgaccacgt tggatgacac ttgtgatctc tttgcccccc tagtccagga 1321 gactaataag cagccccccc agacggtgaa cccaattggc catctcccag aaagtttaac 1381 aaaacccgac caggttgccc ccttcacctt cttacaacct cttccccttt ccccaggggt 1441 cctgtcctcc actccagatc attcctttag tttagagctg ggcagtgaag tggatatcac 1501 tgaaggagat aggaagccag actacactgc ttacgttgcc atgatccctc agtgcataaa 1561 ggaggaagac accccttcag ataatgatag tggcatctgt atgagcccag agtcctatct 1621 ggggtctcct cagcacagcc cctctaccag gggctctcca aataggagcc tcccatctcc 1681 aggtgttctc tgtgggtctg cccgtcccaa accttacgat cctcctggag agaagatggt 1741 agcagcaaaa gtaaagggtg agaaactgga taagaagctg aaaaaaatgg agcaaaacaa 1801 gacagcagcc actaggtacc gccagaagaa gagggcggag caggaggctc ttactggtga 1861 gtgcaaagag ctggaaaaga agaacgaggc tctaaaagag agggcggatt ccctggccaa 1921 ggagatccag tacctgaaag atttgataga agaggtccgc aaggcaaggg ggaagaaaag 1981 ggtcccctgg aattctgcag atatccagca cagtggcggc cgcatggcct cctccgagga 2041 cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga 2101 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 2161 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca 2221 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 2281 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 2341 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 2401 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 2461 caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa 2521 gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc 2581 cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga 2641 ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccta 2701 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 2761 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 2821 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 2881 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 2941 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 3001 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 3061 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 3121 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 3181 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 3241 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 3301 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 3361 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 3421 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 3481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 3541 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3601 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 3661 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 3721 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 3781 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 3841 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 3901 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 3961 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 4021 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 4081 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 4141 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 4201 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 4261 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 4321 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 4381 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4441 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4501 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 4561 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4621 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 4681 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 4741 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 4801 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 4861 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 4921 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 4981 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 5041 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 5101 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 5161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 5221 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5281 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5581 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5821 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 5941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 6061 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 6121 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 6181 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 6241 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 6301 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6361 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 6421 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 6481 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 6541 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 6601 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 6661 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 6721 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6781 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6841 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6901 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6961 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 7021 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 7081 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 7141 ctgacgtc //