LOCUS Exported 12113 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of EGFR L747_A750delinsP. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-L747_A750delinsP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12113) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12113) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12113 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7073..7097) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7105..7123 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7148..7167) /label=T7 /note="T7 promoter, forward primer" promoter complement(7149..7167) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(7173..7193) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 7247..7288 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7309..7876 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7326..7878 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7470..7487) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7697..7716 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7890..8489 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7890..7909) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8386..8406 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8503..9091 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8556..8576) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8974..8985) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9166..9399 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9518..9536) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9604..9708 /gene="bla" /label=AmpR promoter CDS 9709..10569 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9927..9946) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10740..11328 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11229..11248 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11482..11499 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11591..11610 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11739..11760 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11775..11805 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11813..11829 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11818..11840 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11837..11853 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11837..11853 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11903..11922 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc 3481 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc 3541 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc 3601 cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag 3661 aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt 3721 gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg 3781 tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga 3841 ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt gcggttcagc 3901 aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt cagcagtgac 3961 tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca aaagtgtgat 4021 ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca gaaactgacc 4081 aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc 4141 tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg cctggtctgc 4201 cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat gctctacaac 4261 cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg tgccacctgc 4321 gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt 4381 ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg 4441 ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 4501 aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 4561 ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 4621 ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 4681 gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 4741 caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 4801 tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 4861 gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 4921 agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 4981 cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga 5041 ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 5101 aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 5161 acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 5221 gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 5281 gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 5341 ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 5401 ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc 5461 cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg agagggagct tgtggagcct 5521 cttacaccca gtggagaagc tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa 5581 ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca cggtgtataa gggactctgg 5641 atcccagaag gtgagaaagt taaaattccc gtcgctatca aggaaccaac atctccgaaa 5701 gccaacaagg aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg 5761 tgccgcctgc tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc 5821 ttcggctgcc tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg 5881 ctcaactggt gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg 5941 caccgcgacc tggcagccag gaacgtactg gtgaaaacac cgcagcatgt caagatcaca 6001 gattttgggc tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc 6061 aaagtgccta tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag 6121 agtgatgtct ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca 6181 tatgacggaa tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct 6241 cagccaccca tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac 6301 gcagatagtc gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac 6361 ccccagcgct accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac 6421 tccaacttct accgtgccct gatggatgaa gaagacatgg acgacgtggt ggatgccgac 6481 gagtacctca tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc 6541 ctgagctctc tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg 6601 ctgcaaagct gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca 6661 ggcgccttga ctgaggacag catagacgac accttcctcc cagtgcctga atacataaac 6721 cagtccgttc ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca caatcagcct 6781 ctgaaccccg cgcccagcag agacccacac taccaggacc cccacagcac tgcagtgggc 6841 aaccccgagt atctcaacac tgtccagccc acctgtgtca acagcacatt cgacagccct 6901 gcccactggg cccagaaagg cagccaccaa attagcctgg acaaccctga ctaccagcag 6961 gacttctttc ccaaggaagc caagccaaat ggcatcttta agggctccac agctgaaaat 7021 gcagaatacc taagggtcgc gccacaaagc agtgaattta ttggagcata gaacccagct 7081 ttcttgtaca aagtggtgat atccaattaa ccctcactaa agggattagt atttagtagt 7141 aatagagccc tatagtgagt cgtattacca cccaactttt ctatacaaag tggttgatat 7201 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc 7261 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc 7321 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 7381 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 7441 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 7501 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 7561 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 7621 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 7681 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 7741 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt 7801 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg 7861 aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg 7921 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 7981 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 8041 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 8101 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 8161 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 8221 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 8281 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 8341 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 8401 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 8461 ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc tctggattac 8521 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 8581 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 8641 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 8701 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 8761 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 8821 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 8881 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg 8941 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 9001 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 9061 agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca 9121 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 9181 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 9241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 9301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 9361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 9421 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa 9481 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta 9541 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 9601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 9661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 9721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 9781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 9841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 9901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc 9961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 10021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 10081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 10141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 10201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 10261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 10321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 10381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 10441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 10501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 10561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 10621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 10681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 10741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 10801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 10861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 10921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 10981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 11041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 11101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 11161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 11221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 11281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 11341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 11401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 11461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 11521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac 11581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 11641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg 11701 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 11761 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 11821 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca 11881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 11941 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 12001 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 12061 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt //