LOCUS Exported 12996 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette. ACCESSION . VERSION . KEYWORDS AICSDP-63: ACTN2-mEGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12996) AUTHORS Allen Institute for Cell Science TITLE Allen Institute for Cell Science Plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 12996) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12996 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 113..492 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" polyA_signal 508..629 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(519..538) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 573..592 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1732..1749) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1732..1748) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1741..1763) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(1957..1976) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2076..2098 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2136..2154) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2222..2326 /gene="bla" /label=AmpR promoter CDS 2327..3187 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2545..2564) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3358..3946 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3847..3866 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4100..4117 /label=L4440 /note="L4440 vector, forward primer" protein_bind 4234..4255 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4270..4300 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4308..4324 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4313..4335 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4332..4348 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4332..4348 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 4421..4632 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 4645..4913 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 4947..5663 /codon_start=1 /product="enhanced monomeric variant of CFP (Goedhart et al., 2010)" /label=mTurquoise /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5685..5741 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 6570..6626 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(7850..7869) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 7915..7970 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(7969..7988) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" CDS complement(9308..10024) /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" polyA_signal 10105..10329 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" CDS complement(10346..10945) /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(10429..10449) /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind 10926..10945 /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" CDS complement(10946..10999) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" primer_bind complement(11290..11309) /label=mCherry-F /note="mCherry, forward primer" primer_bind 11559..11577 /label=mCherry-R /note="mCherry, reverse primer" primer_bind complement(11784..11803) /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" intron 11811..12828 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind complement(11865..11887) /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" promoter complement(12830..111) /label=chicken beta-actin promoter ORIGIN 1 cccccgcccc catcgctgca caaaataatt aaaaaataaa taaatacaaa attgggggtg 61 gggagggggg ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa 121 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact 181 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca 241 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac 301 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg 361 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga 421 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca 481 ataatcaatg tcgagactca gacatgataa gatacattga tgagtttgga caaaccacaa 541 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 601 taaccattat aagctgcaat aaacaagtta tcggaatact aatataactt cgtatatcct 661 acactatacg aagttattgg tatgtcggga acctctccgg gcccagcagt cccgtcgacc 721 agatcgctct cgccgtacag tgcggaagag aacgcagcgt aatccagtgc accaggcaca 781 ctgcctgggc ccgagtaggc gggcatcctc ttgatgcagt actgggcctg atccgggggc 841 agctcccgac gcagctcctc cgccaggatg tatggctggg aaaaaataca cacgtgtttg 901 cagtcagttg cagaaacacc acaacataaa cagtcttaat acttacatat ttcttgcatc 961 tgtttaattt tttaaaagga ctaaagaata gggagtctat tggcatagta gtaccagaca 1021 accaacagaa agccaagttt tacttgtagg agcgtatcta gaagcggcct ggccagcagg 1081 aagggtgata tgcttcataa aggcaaatgg agcattacta gtagttctca aactgagcca 1141 caatgcgagg atacgtacta tgatctgcat ttcataccag aaaagatgaa gcttttaaaa 1201 aaaaagttca gtgggaaata ggacaattcc agccaaatag tctggttggt aaaagtcaaa 1261 ataagtaaat ctcaaatatc acattataat ttatatcatt atagtttgca tattatataa 1321 tttatagtag aagtttctta gtggggttgg aggagccatg gtttcttacc ttctaaatca 1381 gctgggctat ttcccatcca ccctcacccc atgggtaaat gattcatttt aaagaaaaca 1441 ttattttaaa aatagttggt cttatctgat ttttaaggta ataaatatgg ctctgttact 1501 gtgttttagc atgctaacct ccttgtggta atgttcatta tcctctcagc cagaaaacag 1561 ggtaaaataa tcctgtccca aattgaattt atacatagag atgtttttat tgtttggggg 1621 gtaaaatatt acaattctga actggcaatt aaactcctcc tgaaggtcta ggctacatgt 1681 aatgaaaagc ttgccaattt tttactgact gaacggctaa cgcgtcaatt cactggccgt 1741 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 1801 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 1861 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct 1921 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata 1981 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 2041 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 2101 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 2161 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 2221 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2281 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 2341 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 2401 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 2461 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 2521 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 2581 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 2641 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 2701 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 2761 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 2821 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 2881 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 2941 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3001 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3061 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3121 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3181 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3241 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3301 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3361 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3421 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3481 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 3541 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 3601 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 3661 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 3721 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 3781 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 3841 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 3901 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 3961 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 4021 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 4081 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 4141 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 4201 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 4261 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 4321 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcggcg cgccattggg 4381 atgttaacaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 4441 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 4501 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 4561 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 4621 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4681 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 4741 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 4801 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 4861 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4921 tgaccggcgc ctacctcgag cccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 4981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 5041 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 5101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct 5161 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 5221 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 5281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 5341 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc gacaacgtct 5401 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca 5461 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 5521 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc 5581 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 5641 tcggcatgga cgagctgtac aagggtgcta gcaccggaag cggagctact aacttcagcc 5701 tgctgaagca ggctggagac gtggaggaga accctggacc tactatgaaa aagcctgaac 5761 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga 5821 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat 5881 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc 5941 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga 6001 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa 6061 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg 6121 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta 6181 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga 6241 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg 6301 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga 6361 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc 6421 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga 6481 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cggggcgtat 6541 atgctccgca ttggtctagt ggctcaggcg caacaaactt ctctctcctc aaacaagccg 6601 gcgatgtcga agagaaccca ggcccaatgg cttcttaccc tggacaccag catgcttctg 6661 cctttgacca ggctgccaga tccaggggcc actccaacag gagaactgcc ctaagaccca 6721 gaagacagca ggaagccact gaggtgaggc ctgagcagaa gatgccaacc ctgctgaggg 6781 tgtacattga tggacctcat ggcatgggca agaccaccac cactcaactg ctggtggcac 6841 tgggctccag ggatgacatt gtgtatgtgc ctgagccaat gacctactgg agagtgctag 6901 gagcctctga gaccattgcc aacatctaca ccacccagca caggctggac cagggagaaa 6961 tctctgctgg agatgctgct gtggtgatga cctctgccca gatcacaatg ggaatgccct 7021 atgctgtgac tgatgctgtt ctggctcctc acattggagg agaggctggc tcttctcatg 7081 cccctccacc tgccctgacc ctgatctttg acagacaccc cattgcagcc ctgctgtgct 7141 acccagcagc aaggtacctc atgggctcca tgaccccaca ggctgtgctg gcttttgtgg 7201 ccctgatccc tccaaccctc cctggcacca acattgttct gggagcactg cctgaagaca 7261 gacacattga caggctggca aagaggcaga gacctggaga gagactggac ctggccatgc 7321 tggctgcaat cagaagggtg tatggactgc tggcaaacac tgtgagatac ctccagtgtg 7381 gaggctcttg gagagaggac tggggacagc tctctggaac agcagtgccc cctcaaggag 7441 ctgagcccca gtccaatgct ggtccaagac cccacattgg ggacaccctg ttcaccctgt 7501 tcagagcccc tgagctgctg gctcccaatg gagacctgta caatgtgttt gcctgggctc 7561 tggatgttct agccaagagg ctgaggtcca tgcatgtgtt catcctggac tatgaccagt 7621 cccctgctgg atgcagagat gctctgctgc aactaacctc tggcatggtg cagacccatg 7681 tgaccacccc tggcagcatc cccaccatct gtgacctagc cagaaccttt gccagggaga 7741 tgggagaggc caactaagct agctagaccg gtactcctca ggtgcaggct gcctatcaga 7801 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 7861 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 7921 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 7981 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 8041 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 8101 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 8161 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 8221 ctgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 8281 ttctcttatg gagatcgtta acatttaaat ctctcctact ctaactctgg cttctcctta 8341 accggtcata aaactagagg cataaggaag tcctgactgg agagctagaa ggatactggt 8401 ttctgacttg ccttgtgatg aaccagtcag tactacagaa acctgcttat ttataaacag 8461 agcactttta tgtggtcatc tcttgagaac ctgtactaat aagctctaac aaaaagcatg 8521 ggaacctgga atcaataaat aatcctaaaa acctcatttt acctagtgat tgcatccttc 8581 tctctggcaa ttaaactgac ttccacgtgt ccttaggtct ctattctgga ggataagaga 8641 agcatcaaaa aatcactgac tgagttgacc atccagccct aaataatgaa gagatgacat 8701 aaactatttt aaaaattgta agtgccaaaa atcttttggg tagtcttttt aatccccggc 8761 agttccatct cacaatatta aattcatttg aagatacaat agtaatcttt ggataagcat 8821 gaaaataaaa cattagaatc ccaaatatac tcccctaggg gcaatcacat aagcatatta 8881 gagataaatg aaatttttta caaatagcaa acctttattt accaatttgt ggttttttcc 8941 ccccggtctt aaatcttggg tattttgtat tttcacattt ttcactttcc ttctgtccct 9001 agatcctcct aatttcctat tactcaagta atttgttctg tttaatcaaa gaaatcaacc 9061 tgatatttca ttacattttg ctaaaaactc agaaataaac ctatttatga agcgcactat 9121 gtcatatact gtaaaaactt cattttagcc gtgttatatt gctggcaatt actgaactac 9181 gaaaggcttt tttttttccc ctctctctct ctcaacttaa taaagcttgt caaagtttcc 9241 aggaaacaaa ctgtgacttc cgcttttatt gcattctgat gccatgagtg attacagaag 9301 ctcagcatca cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact 9361 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc 9421 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt 9481 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct 9541 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact 9601 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc 9661 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt 9721 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt 9781 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg 9841 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca 9901 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta 9961 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc 10021 tcacagtagc aatggatccg ggcccggaga ggttcccgac atacctatat aacttcgtat 10081 atcctacact atacgaagtt atttccatag agcccaccgc atccccagca tgcctgctat 10141 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac 10201 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg 10261 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag 10321 aaggcacagg agtacctcac ttaagtcagg caccgggctt gcgggtcatg caccaggtgc 10381 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg 10441 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct 10501 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagactc 10561 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca 10621 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg 10681 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt 10741 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca 10801 gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt 10861 cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc 10921 gcaccgtggg cttgtactcg gtcattgggc caggattctc ctcgacgtca ccgcatgtga 10981 gcagacttcc tctgccctcc ttaagtgcgg ccgctttata gagttcatcc attccgccgg 11041 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg 11101 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg 11161 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct 11221 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc 11281 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct 11341 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc 11401 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca 11461 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag 11521 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg 11581 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt 11641 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat 11701 cttcttctcc tttggagacc atggtaagct cttcgaacgg tggatccccg gtggcggaat 11761 tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa 11821 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag 11881 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga 11941 ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt 12001 tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc 12061 ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac 12121 gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg 12181 gggccgccgc gccccccccc cgagcccccc ccggcccgag gcggccccgc cccgcccggc 12241 acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg 12301 agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt 12361 tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg 12421 cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc 12481 ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg 12541 ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc 12601 gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac 12661 agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg 12721 tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag 12781 gcggcggcgg agcggggcac ggggcgaagg cagcgtcgca gcgactcccc gcccgccgcg 12841 cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga 12901 gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc 12961 cccgccccgc cccgcctggc gcgcgccccc cccccc //