LOCUS Exported 10592 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cre/Lox C-terminal tagging construct encoding glutathione S-transferase. ACCESSION . VERSION . KEYWORDS pAW8_GST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10592) AUTHORS Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J. TITLE Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe. JOURNAL Gene. 2008 Jan 15;407(1-2):63-74 PUBMED 18054176 REFERENCE 2 (bases 1 to 10592) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10592 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 575..1356 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" primer_bind complement(1451..1470) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1570..1592 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1630..1648) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1716..1820 /gene="bla" /label=AmpR promoter CDS 1821..2681 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2039..2058) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2852..3440 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3341..3360 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3594..3611 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3728..3749 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3764..3794 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3802..3818 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3807..3829 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3826..3842 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3826..3842 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind 3874..3907 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 3916..4569 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" primer_bind 4527..4549 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" terminator 4617..4804 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(4672..4696) /label=pHybLex-R /note="ADH terminator, reverse primer" gene 4879..6235 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 4879..5222 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 5223..6032 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(5290..5309) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 6038..6235 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(6330..6347) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6330..6346) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6339..6361) /label=M13/pUC Forward /note="In lacZ gene" CDS 9241..10335 /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" ORIGIN 1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac 781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3901 aagttatgca tgctcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa 3961 cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc 4021 gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt 4081 ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata 4141 gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt 4201 gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt 4261 gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat 4321 cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg 4381 tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa 4441 ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc 4501 agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat 4561 cctccaaaat cggatctggt tccgcgttga ggcgcgccag atctacttct aaataagcga 4621 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca 4681 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc 4741 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta 4801 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg 4861 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg 4921 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta 4981 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc 5041 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac 5101 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat 5161 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa 5221 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg 5281 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 5341 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 5401 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 5461 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 5521 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 5581 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 5641 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 5701 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 5761 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 5821 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 5881 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 5941 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 6001 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt 6061 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 6121 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 6181 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 6241 gattcgatac taacgccgcc atccagtcga cgaactagta taacttcgta tatggtatta 6301 tatacgaagt tatgcggccg cgcgaattca ctggccgtcg ttttacaacg tcgtgactgg 6361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 6421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 6481 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 6541 ccatcgtccg gatcaatgaa gtagatgagt ttactacctg tatatacttt tttttttgtc 6601 aaagtcactt ttatggcatt tccatgttga aggattagcc acagcactaa attttcagcg 6661 tatgattgct tttaaatatt taattttcat cgttttttaa ttaatattca aaacgattta 6721 atgccttcta catctgaagt caagctcata gactcgggtt gggtagctaa aactacatct 6781 aacacgaagg ggtatagcct tcttaaagta tactcatgta taaacgtttt gggttcatat 6841 ttttgggcca cgtggtttat aaaaattctt aactacacca ctcggtatcc cgcacccgtc 6901 tacgtttcta cgatttcgag ctaatattga gtaaagtgat tagcaaaaaa aataaaagta 6961 ctcgttgtcg gagatcaaga atttttctat tatctcatct aaaccacttt ctaaaagcga 7021 aaaacaaaat cgtaatatgc agcttgaatg ggcttccata gtttgaaaga aaaaccctag 7081 cagtactggc aagggagaca ttccttttac ctggctaatc gccatcttcc agcaggcgca 7141 ccattgcccc tgtttcacta tccaggttac ggatatagtt catgacaata tttacattgg 7201 tccagccacc agcttgcatg atctccggta ttgaaactcc agcgcgggcc atatctcgcg 7261 cggctccgac acgggcactg tgtccagacc aggccaggta tctctgacca gagtcatcct 7321 tagcgccgta aatcaatcga tgagttgctt caaaaatccc ttccagggcg cgagttgata 7381 gctggctggt ggcagatggc gcggcaacac cattttttct gacccggcaa aacaggtagt 7441 tattcggatc atcagctaca ccagagacgg aaatccatcg ctcgaccagt ttagttaccc 7501 ccaggctaag tgccttctct acacctgcgg tgctaaccag cgttttcgtt ctgccaatat 7561 ggattaacat tctcccaccg tcagtacgtg agatatcttt aaccctgatc ctggcaattt 7621 cggctatacg taacagggtg ttataagcaa tccccagaaa tgccagatta cgtatatcct 7681 ggcagcgatc gctattttcc atgagtgaac gaacctggtc gaaatcagtg cgttcgaacg 7741 ctagagcctg ttttgcacgt tcaccggcat caacgttttc ttttcggatc cgccgcataa 7801 ccagtgaaac agcattgctg tcacttggtc gtggcagccc ggaccgacga tgaagcatgt 7861 ttagctggcc caaatgttgc tggatagttt ttactgccag accgcgcgcc tgaagatata 7921 gaagataatc gcgaacatct tcaggttctg cgggaaacca tttccggtta ttcaacttgc 7981 accatgccgc ccacgaccgg caaacggaca gaagcatttt ccaggtatgc tcagaaaacg 8041 cctggcgatc cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga 8101 ccggtaactt ttagattaac atttgtttaa atatgaaatt aggcaatgta acatactgca 8161 ggcaaatttt ggtgtacggt aagtaaattg gacatatgat ttaacaaagc gactataagt 8221 cagaaagtga gaatgagatt gaaataatta attcaactta ttcaattgat gatatgccag 8281 gattcctctt cctttatctt caatgtttcg attaacaggt ccattatccg gtttttagtt 8341 tcgctgcaca ttgccgaatg acaatcggat tcctttaagc tttgcttttg ccctgttttc 8401 tctccccagt aatcgttggg tgactgaacc atccagtagc cgagagttac gtttttcctg 8461 atttaataaa tatccatatc tatttgtctc gtggtgtttt acagcaacta accgaaatta 8521 ctattttgca tcatccaacc ataaagagac aacaataatt ctagaaaaca tataacaagt 8581 gtttttttct atttacgtct catacgcaaa gtccacttta ccatgttttg gaaacttgta 8641 cccaaccatt catttttcga ttttgtaatg aggctctttt cttctaacaa tcattgaggt 8701 cattatcatg tgtcctcacc tattgtttaa attgaggctt gcgctattcc caaaattgtg 8761 ggtattattt tcatatatca gtatctgcaa tttcggttca actccggaag ctcggtaccc 8821 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta 8881 ttttttttct ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg 8941 atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg 9001 aaaaattggg agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga 9061 gaagcgttca tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg 9121 acctattgtt ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct 9181 tttacatttc agcaatatat atatatatat atatatatat ttcaaggata taccattgta 9241 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca 9301 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat 9361 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat 9421 gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct 9481 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt 9541 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct 9601 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgttagaga attagtggga 9661 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa 9721 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa 9781 catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga 9841 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat ttcctacatt gaaagttcaa 9901 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt 9961 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca 10021 ggctccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca 10081 tttggtttgt acgaaccatg ccatggttcc gctccagatt tgccaaagaa taaggtcaac 10141 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa 10201 gaaggtaaag ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt 10261 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag 10321 aaaatccttg cttaaaaaga ttctcttttt ttgtgatatt tgtacataaa ctttataaat 10381 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga 10441 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa 10501 tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta 10561 acattaatat tgacaaggag gagggcatca ca //