LOCUS Exported 11866 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression. ACCESSION . VERSION . KEYWORDS pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11866) AUTHORS Suzuki IK, Gacquer D, Van Heurck R, Kumar D, Wojno M, Bilheu A, Herpoel A, Lambert N, Cheron J, Polleux F, Detours V, Vanderhaeghen P TITLE Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation. JOURNAL Cell. 2018 May 31;173(6):1370-1384.e16. doi: 10.1016/j.cell.2018.03.067. Epub 2018 May 31. PUBMED 29856955 REFERENCE 2 (bases 1 to 11866) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11866 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2602..2905 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" intron 3185..4201 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 4125..4147 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 4209..4228 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 4264..4283 /label=T7 /note="T7 promoter, forward primer" promoter 4264..4282 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 4297..4315 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 4297..4314 /label=SP6 /note="SP6 promoter, forward primer" CDS 5181..5198 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 5228..5814 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5395..5412) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5622..5641 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5815..6534 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5815..6531 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5860..5881) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6121..6140) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6468..6489 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6558..7146 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6611..6631) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7029..7040) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7149..7165) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7150..7166) /label=pBluescriptKS /note="For pBluescript vector" LTR 7671..7851 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7877..7894) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7883..8107 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8153..8581 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8240..8259) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8450..8471 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8590..8610) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8595..8924 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8775..8910 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8837..8856 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8972..9019 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9038..9412 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9542..9663 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9579..9598) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9633..9652 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9712..9728) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9712..9728) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9725..9747) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9736..9752 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9760..9790) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9805..9826 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9943..9960) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10114..10702) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10194..10213) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10873..11733) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11496..11515 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11734..11838) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc 2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 3001 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsggggg 3061 gsggggsggg gsgrggsggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa 3121 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 3181 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg 3241 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 3301 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 3361 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 3421 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 3481 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 3541 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 3601 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 3661 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 3721 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 3781 ggcggggccg ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc 3841 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag 3901 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg 3961 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg 4021 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg 4081 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg 4141 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca 4201 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgctcga 4261 gcgtaatacg actcactata gggagaccca agcttgattt aggtgacact atagaataca 4321 agctacttgt tctttttgca ggatccgacc gagagatgcc cgccctgcgc cccgctctgc 4381 tgtgggcgct gctggcgctc tggctgtgct gcgcgacccc cgcgcatgca ttgcagtgtc 4441 gagatggcta tgaaccctgt gtaaatgaag gaatgtgtgt tacctaccac aatggcacag 4501 gatactgcaa atgtccagaa ggcttcttgg gggaatattg tcaacatcga gacccctgtg 4561 agaagaaccg ctgccagaat ggtgggactt gtgtggccca ggccatgctg gggaaagcca 4621 cgtgccgatg tgcctcaggg tttacaggag aggactgcca gtactcgaca tctcatccat 4681 gctttgtgtc tcgaccttgc ctgaatggcg gcacatgcca tatgctcagc cgggatacct 4741 atgagtgcac ctgtcaagtc gggtttacag gtaaggagtg ccaatggacc gatgcctgcc 4801 tgtctcatcc ctgtgcaaat ggaagtacct gtaccactgt ggccaaccag ttctcctgca 4861 aatgcctcac aggcttcaca gggcagaagt gtgagactga tgtcaatgag tgtgacattc 4921 caggacactg ccagcatggt ggcatctgcc tcaacctgcc tggttcctac cagtgccagt 4981 gccttcaggg cttcacaggc cagtactgtg acagcctgta tgtgccctgt gcaccctcgc 5041 cttgtgtcaa tggaggcacc tgtcggcaga ctggtgactt cacttttgag tgcaactgcc 5101 ttccagaaac agtgagaaga ggaacagagc tctgggaaag agacagggaa gtctggaatg 5161 gaaaagaaca cgatgagaat caccaccatc atcaccacta gttaattaag ctagcgttaa 5221 cgatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 5281 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 5341 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 5401 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 5461 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 5521 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 5581 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 5641 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 5701 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 5761 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg 5821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 5881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 5941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 6001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 6061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 6121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 6181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 6241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 6301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 6361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 6421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 6481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaaattcg 6541 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta 6601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 6661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 6721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 6781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 6841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 6901 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt 6961 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 7021 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 7081 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 7141 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca 7201 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg 7261 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc 7321 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 7381 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact 7441 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac 7501 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc 7561 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca 7621 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct 7681 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 7741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 7801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt 7861 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 7921 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 7981 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 8041 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 8101 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 8161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 8221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 8281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 8341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 8401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 8461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 8521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 8581 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 8641 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 8701 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 8761 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 8821 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 8881 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 8941 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat 9001 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt 9061 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg 9121 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct 9181 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt 9241 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga 9301 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct 9361 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct 9421 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 9481 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 9541 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 9601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 9661 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 9721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 9781 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 9841 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 9901 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 9961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 10021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 10081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 10141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 10201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 10261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 10321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 10381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 10441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 10501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 10561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 10621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 10681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 10741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 10801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 10861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 10921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 10981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 11041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 11101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 11161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 11221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 11281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 11341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 11401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 11461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 11521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 11581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 11641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 11701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 11761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 11821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac //