LOCUS Exported 12189 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator. ACCESSION . VERSION . KEYWORDS pEGB 35s:dCas:EDLL:tNos (GB1190) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12189) AUTHORS Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. JOURNAL Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. PUBMED 26839579 REFERENCE 2 (bases 1 to 12189) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12189 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 616..960 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 904..924 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 1037..1057 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1058..5158 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5177..5197 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5201..5221 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5225..5245 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 5344..5596 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(5631..5649) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 5833..5857 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 7158..7787 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 8216..9289 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9355..9549 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(9785..9807) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 9893..10033 /label=bom /note="basis of mobility region from pBR322" primer_bind 9948..9967 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(10048..10065) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10219..10807) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10299..10318) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10895..11710) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 11618..11637 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" misc_feature 12143..12167 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta 61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga 121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga 181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa 241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa 301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg 361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac 421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa 481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt 541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga 601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt 661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta 721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg 781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac 841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc 901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac 961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac 1021 tattctagtc gaaatgccca agaagaagag gaaggtggac aagaagtact ccattgggct 1081 cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag 1141 caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg 1201 cgccctcctg ttcgactccg gggaaacggc cgaagccacg cggctcaaaa gaacagcacg 1261 gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga 1321 gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga 1381 ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca 1441 tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc 1501 tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct 1561 catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt 1621 tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa 1681 agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct 1741 ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac 1801 ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga 1861 cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct 1921 ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa 1981 cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca 2041 ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga 2101 aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca 2161 ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct 2221 gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag 2281 catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt 2341 ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc 2401 ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc 2461 agaagagact atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca 2521 gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc 2581 taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata 2641 cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt 2701 ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagattattt 2761 caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc 2821 atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa 2881 tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag 2941 ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa 3001 acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg 3061 gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc 3121 caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca 3181 gaaagcacaa gtttctggcc agggggacag tctccacgag cacatcgcta atcttgcagg 3241 tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa 3301 agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac 3361 tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa 3421 agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga 3481 gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga 3541 catcaatcgg ctctccgact acgacgtgga tgccatcgtg ccccagtctt ttctcaaaga 3601 tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa 3661 cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc 3721 caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc 3781 tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa 3841 gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact 3901 gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtttcagatt tcagaaagga 3961 ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct 4021 gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt 4081 ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat 4141 aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga 4201 gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac 4261 aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat 4321 gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag 4381 tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa 4441 gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt 4501 ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat 4561 ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga 4621 ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg 4681 ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc 4741 ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aaggatctcc 4801 cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat 4861 catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa 4921 ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat 4981 tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac 5041 caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca 5101 tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag 5161 cagggctgat tcggatccta aaaagaagcg aaaggtagac ccgaagaaaa agaggaaggt 5221 agatccaaag aagaagcgca aggtagaagt gtttgaattt gaatatctgg atgataaagt 5281 gctggaagaa ctgctggata gcgaagaacg caaacgctga gcttggaatg gatcttcgat 5341 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 5401 gcgacgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 5461 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa 5521 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 5581 tctatgttac tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat 5641 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 5701 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 5761 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct 5821 tgagacgaag cttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta 5881 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc 5941 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta 6001 tagtgcagtc ggcttctgac gttcagtgca gccgtcatct gaaaacgaca tgtcgcacaa 6061 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg 6121 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa 6181 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac 6241 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac 6301 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga 6361 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat 6421 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga 6481 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga 6541 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt 6601 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat 6661 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac 6721 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg 6781 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg 6841 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc 6901 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacctc 6961 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg 7021 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt 7081 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa 7141 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca 7201 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt 7261 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac 7321 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc 7381 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga 7441 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca 7501 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag 7561 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg 7621 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc 7681 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc 7741 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa 7801 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag 7861 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca 7921 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat 7981 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa 8041 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca 8101 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct 8161 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag 8221 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 8281 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 8341 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 8401 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 8461 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 8521 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 8581 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 8641 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 8701 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 8761 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 8821 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 8881 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 8941 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 9001 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 9061 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 9121 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 9181 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 9241 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 9301 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggactct ttcctgtgga 9361 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 9421 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 9481 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 9541 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 9601 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 9661 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 9721 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 9781 aaaacctctg acacatgcag ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg 9841 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 9901 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 9961 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 10021 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 10081 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 10141 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 10201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 10261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 10321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 10381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 10441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 10501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 10561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 10621 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 10681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 10741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 10801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 10861 acgttaaggg attttggtca tgcattctag gtgattagaa aaactcatcg agcatcaaat 10921 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct 10981 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt 11041 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa 11101 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt 11161 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac 11221 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat 11281 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca 11341 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt 11401 tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga 11461 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat 11521 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat 11581 acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat 11641 ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa 11701 tatggctcat aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc 11761 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt 11821 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg 11881 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat 11941 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc 12001 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga 12061 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt 12121 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacata acaagcttcg 12181 tctcagtca //