LOCUS Exported 2300 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D). ACCESSION . VERSION . KEYWORDS pCAG-mEGFP-CaMKIIa (T305D/T306D) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2300) AUTHORS Chang JY, Nakahata Y, Hayano Y, Yasuda R TITLE Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in single dendritic spines. JOURNAL Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z. PUBMED 31239443 REFERENCE 2 (bases 1 to 2300) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2300 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 50..59 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 56..772 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(101..122) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(362..381) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 709..730 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(2257..2276) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" ORIGIN 1 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgctagcg ccaccatggt 61 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 121 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 241 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 481 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 541 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 661 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 721 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact 781 cagatctcga gctatggcta ccatcacctg cacccgattc acggaagagt accagctctt 841 cgaggaactg ggaaagggag ccttctccgt ggtgcgcagg tgtgtgaagg tgctggctgg 901 ccaggagtat gctgccaaga ttatcaacac caagaagctc tcagccagag atcaccagaa 961 gttggaacgc gaggcccgca tctgccgctt gttgaagcac cccaatatcg tccgactcca 1021 tgacagcatc tccgaggagg ggcaccacta ccttatcttc gatctggtca ctggtgggga 1081 gctgttcgaa gacattgtgg cccgggagta ttacagtgag gctgatgcca gccactgtat 1141 ccagcagatc ctggaggctg tgctacactg tcaccagatg ggggtggtgc atcgcgacct 1201 gaagcctgag aatctgttgc tggcttcgaa gctcaagggt gctgcggtga agctggcaga 1261 ctttggcctg gccatagagg ttgagggaga gcagcaggca tggtttgggt tcgcagggac 1321 acctggatac ctctccccag aagtgctgcg gaaggaccca tacgggaagc ctgtggacct 1381 gtgggcctgt ggcgtcatcc tgtatatctt gctggttggg tatcccccat tctgggatga 1441 ggaccagcac cgcctgtacc agcagatcaa agctggtgcc tacgatttcc catcaccaga 1501 atgggacacc gtcaccccgg aagccaagga tctgatcaat aagatgctga ccatcaaccc 1561 gtccaaacgc atcacggccg ctgaggctct caagcacccc tggatctcgc accgctccac 1621 tgtggcctcc tgcatgcaca gacaggagac cgtggactgc ctgaagaagt tcaatgccag 1681 gaggaaactg aagggagcca tcctcgacga tatgctggcc accaggaact tctccggagg 1741 gaagagtgga ggaaacaaga agaatgatgg cgtgaaggaa tcctctgaga gcaccaacac 1801 caccatcgag gatgaagaca ccaaagtgcg caaacaggaa attatcaaag tgacagagca 1861 gctgatcgaa gccataagca atggagactt tgaatcctac acgaagatgt gcgaccctgg 1921 aatgacagcc tttgaaccgg aggccctggg gaacctggtc gagggcctgg actttcatcg 1981 attctatttt gaaaacctgt ggtcccggaa cagcaagccc gtgcacacca ccatcctgaa 2041 ccctcacatc cacctgatgg gtgacgagtc agcctgcatc gcctacatcc gcatcactca 2101 gtacctggat gcgggtggca tcccccgcac ggcccagtca gaggagaccc gtgtctggca 2161 ccgcagggat ggaaaatggc agatcgtcca cttccacaga tctggggcgc cctccgtcct 2221 gccccattga gcggccgcga ctctagagtc gacggatctt tttccctctg ccaaaaatta 2281 tggggacatc atgaagcccc //