LOCUS Exported 8979 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.. ACCESSION . VERSION . KEYWORDS pLenti PGK Puro GFP-LC3B G120A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8979) AUTHORS Agrotis A, Pengo N, Burden JJ, Ketteler R TITLE Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. JOURNAL Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. PUBMED 30661429 REFERENCE 2 (bases 1 to 8979) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8979 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 26..130 /gene="bla" /label=AmpR promoter CDS 131..991 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(349..368) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1162..1750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1651..1670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1904..1921 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2038..2059 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2074..2104 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2112..2128 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2117..2139 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2136..2152 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2136..2152 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2171..2191 /label=T3 /note="T3 promoter, forward primer" promoter 2173..2191 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2219..2445 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2446..2626 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2673..2798 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3291..3524 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3709..3753 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4020..4137 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 4183..4693 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 4612..4630 /label=hPGK-F /note="Human PGK promoter, forward primer" regulatory 4702..4711 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4708..5424 /codon_start=1 /product="green fluorescent protein" /label=GFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" primer_bind complement(4753..4774) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5014..5033) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5361..5382 /label=EGFP-C /note="EGFP, forward primer" protein_bind 5446..5470 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5473..5847 /codon_start=1 /gene="MAP1LC3B" /product="human LC3 protein" /label=LC3B /note="Atg8 family member found on autophagosomes" /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD EDGFLYMVYASQETFAMKLSV" misc_feature 5857..6445 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5910..5930) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6328..6339) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 6459..6958 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6494..6517) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6891..6910 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6979..7578 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6979..6998) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7475..7495 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7716..7949 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8021..8142 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8058..8077) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8112..8131 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8182..8317 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8244..8263 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8337..8356) /label=T7 /note="T7 promoter, forward primer" promoter complement(8338..8356) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8366..8383) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8366..8382) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8375..8397) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8524..8979 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8611..8630) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8821..8842 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta 2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat 3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc 4141 gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc 4921 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 5161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 5281 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac 5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 5401 cacggcatgg acgagctcta caaaagcggt tccggaccgg atcaaacaag tttgtacaaa 5461 aaagcaggct taatgccgtc ggagaagacc ttcaagcagc ggcgcacctt cgaacaaaga 5521 gtagaagatg tccgacttat tcgagagcag catccaacca aaatcccggt gataatagaa 5581 cgatacaagg gtgagaagca gcttcctgtt ctggataaaa caaagttcct tgtacctgac 5641 catgtcaaca tgagtgagct catcaagata attagaaggc gcttacagct caatgctaat 5701 caggccttct tcctgttggt gaacggacac agcatggtca gcgtctccac accaatctca 5761 gaggtgtatg agagtgagaa agatgaagat ggattcctgt acatggtcta tgcctcccag 5821 gagacgttcg cgatgaaatt gtcagtgtaa gtcgacaatc aacctctgga ttacaaaatt 5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6241 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 6421 atctcccttt gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc 6481 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 6541 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 6601 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 6661 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 6721 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 6781 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 6841 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 6901 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 6961 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 7021 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 7081 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 7141 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 7201 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 7261 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 7321 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 7381 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 7441 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7501 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 7561 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca 7621 cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt 7681 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg 7741 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 7801 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7861 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7921 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7981 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 8041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 8101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 8161 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 8221 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 8281 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct 8341 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa 8401 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 8461 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 8521 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 8581 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 8641 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 8701 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 8761 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 8821 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 8881 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 8941 ttaacgcgaa ttttaacaaa atattaacgc ttacaattt //