LOCUS Exported 9822 bp ds-DNA circular SYN 03-NOV-2023 DEFINITION N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.. ACCESSION . VERSION . KEYWORDS pLVpuro-CMV-N-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9822) AUTHORS Agrotis A, Pengo N, Burden JJ, Ketteler R TITLE Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. JOURNAL Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. PUBMED 30661429 REFERENCE 2 (bases 1 to 9822) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 3, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9822 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 23..611 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 512..531 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 1075..1301 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 1302..1482 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1529..1654 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 2147..2380 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2565..2609 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" primer_bind 2907..2927 /label=hU6-F /note="Human U6 promoter, forward primer" promoter 3216..3419 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3370..3390 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" promoter 3464..3482 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3519..3528 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3525..4241 /codon_start=1 /product="green fluorescent protein" /label=GFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" primer_bind complement(3570..3591) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3831..3850) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4178..4199 /label=EGFP-C /note="EGFP, forward primer" protein_bind 4263..4387 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4412..4442 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 4496..5155 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(4562..4581) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 5476..5781 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 5660..5679 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(5822..5946) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 5995..6112 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 6161..6671 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 6590..6608 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 6693..7292 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6693..6712) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7189..7209 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7420..7653 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7725..7846 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7762..7781) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7816..7835 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7886..8021 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7948..7967 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter complement(8042..8060) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8070..8087) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8070..8086) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8079..8101) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8228..8683 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8315..8334) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8525..8546 /label=F1ori-F /note="F1 origin, forward primer" promoter 8709..8813 /gene="bla" /label=AmpR promoter CDS 8814..9674 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9032..9051) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 841 aatgcagctg gcttaatgta gtcttatgca atactcttgt agtcttgcgc aatttcctgc 901 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 961 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 1021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc ttaatgtagt 1081 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac 1141 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct 1201 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt 1261 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc 1321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 1381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 1441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 1501 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 1561 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 1621 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 1681 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 1741 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 1801 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 1861 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 1921 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac 1981 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga 2041 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca 2101 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc 2161 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac 2221 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg 2281 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 2341 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg 2401 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg 2461 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa 2521 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat 2581 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt 2641 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg 2701 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc 2761 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga 2821 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact 2881 agcctcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc 2941 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga 3001 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa 3061 aattatgttt taaaatggac tatcatatgc caagtacgcc ccctattgac gtcaatgacg 3121 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 3181 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 3241 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 3301 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 3361 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 3421 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata 3481 gggagaccca agctggctag cttaattaag actctagagc caccatggtg agcaagggcg 3541 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 3601 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 3661 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 3721 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 3781 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 3841 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 3901 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 3961 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 4021 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 4081 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 4141 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 4201 ccgccgccgg gatcactcac ggcatggacg agctctacaa aagcggttcc ggaccggatc 4261 aaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat 4321 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt 4381 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa 4441 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga 4501 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 4561 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 4621 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 4681 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct 4741 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 4801 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 4861 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 4921 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 4981 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 5041 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 5101 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg 5161 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctc gcgaaccggt 5221 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt 5281 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa 5341 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc 5401 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga 5461 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 5521 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 5581 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 5641 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 5701 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 5761 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga 5821 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 5881 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa 5941 agtggtgtga ttcgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa 6001 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 6061 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 6121 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt 6181 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac 6241 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga 6301 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg 6361 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta 6421 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg 6481 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc 6541 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat 6601 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc 6661 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc 6721 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 6781 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 6841 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 6901 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 6961 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 7021 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 7081 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 7141 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 7201 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 7261 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga 7321 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag 7381 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 7441 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 7501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 7561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 7621 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 7681 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 7741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 7801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 7861 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 7921 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 7981 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg 8041 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 8101 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 8161 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 8221 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 8281 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 8341 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 8401 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 8461 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 8521 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 8581 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 8641 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg 8701 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 8761 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 8821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 8881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 8941 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 9001 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 9061 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 9121 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 9181 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 9241 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 9301 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 9361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 9421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 9481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 9541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 9601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 9661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 9721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 9781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cg //