LOCUS       Exported               12677 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral plasmid for expressing human codon-optimized AsCpf1 
            containing 3x N-terminal MYC-NLS signal.
ACCESSION   .
VERSION     .
KEYWORDS    LentiAsCpf1-3xMYC-blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W,
            Deem A, Bristow C, Hart T, Draetta G
  TITLE     Pooled library screening with multiplexed Cpf1 library.
  JOURNAL   Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x.
  PUBMED    31316073
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2869..2878
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2881..2907
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2908..2934
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2935..2961
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2962..6879
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      6599..6608
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6889..6945
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6946..7344
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7369..7957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7422..7442)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7840..7851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7960..7976)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7961..7977)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8482..8662
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8688..8705)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8694..8918
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8964..9392
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9051..9070)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9261..9282
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9401..9421)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9406..9735
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9586..9721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9648..9667
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9783..9830
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9849..10223
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10353..10474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10390..10409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10444..10463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10523..10539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10523..10539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10536..10558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10547..10563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10571..10601)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10616..10637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10754..10771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10925..11513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11005..11024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11684..12544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12307..12326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12545..12649)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc caccatggcg
     2881 ccagcggcaa agcgcgtaaa attggaccca gcagccaaac gagtaaaact cgatccggct
     2941 gctaaacgag ttaagctgga cacacagttc gagggcttta ccaacctgta tcaggtgagc
     3001 aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat ccaggagcag
     3061 ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa gcccatcatc
     3121 gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct ggattgggag
     3181 aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac aaggaacgcc
     3241 ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat cggccggaca
     3301 gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg cctgttcaag
     3361 gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac aaccgagcac
     3421 gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg cttttatgag
     3481 aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca ccgcatcgtg
     3541 caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct gatcaccgcc
     3601 gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat cttcgtgagc
     3661 acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac acagacccag
     3721 atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac cgagaagatc
     3781 aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac agcccacatc
     3841 atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc cgataggaac
     3901 accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca gtccttctgc
     3961 aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc cctgtttaac
     4021 gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct ggagacaatc
     4081 agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga gcggagaatc
     4141 tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg cagcctgaag
     4201 cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct gagcgaggcc
     4261 ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga tcagccactg
     4321 cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct ggacagcctg
     4381 ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga ggtggacccc
     4441 gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct gagcttctac
     4501 aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt caagctgaac
     4561 tttcagatgc ctacactggc ctctggctgg gacgtgaata aggagaagaa caatggcgcc
     4621 atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca gaagggcagg
     4681 tataaggccc tgagcttcga gcccacagag aaaaccagcg agggctttga taagatgtac
     4741 tatgactact tccctgatgc cgccaagatg atcccaaagt gcagcaccca gctgaaggcc
     4801 gtgacagccc actttcagac ccacacaacc cccatcctgc tgtccaacaa tttcatcgag
     4861 cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga gccaaagaag
     4921 tttcagacag cctacgccaa gaaaaccggc gaccagaagg gctacagaga ggccctgtgc
     4981 aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac ctctatcgat
     5041 ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta tgccgagctg
     5101 aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat catggatgcc
     5161 gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc caagggccac
     5221 cacggcaagc ctaatctgca cacactgtat tggaccggcc tgttttctcc agagaacctg
     5281 gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc taagtccagg
     5341 atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct gaaggatcag
     5401 aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa tcacagactg
     5461 tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac caaggaggtg
     5521 tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt ccacgtgcct
     5581 atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag ggtgaatgcc
     5641 tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga gagaaacctg
     5701 atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag cctgaacacc
     5761 atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag ggtggcagca
     5821 aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta tctgagccag
     5881 gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt gctggagaac
     5941 ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt gtaccagcag
     6001 ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta tccagcagag
     6061 aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc ctttgccaag
     6121 atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc taagatcgat
     6181 cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca cgagagccgc
     6241 aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg cgacttcatc
     6301 ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg ctttatgcct
     6361 gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg cacccctttc
     6421 atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg cagataccgg
     6481 gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat cgtgttcagg
     6541 gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc catcgacacc
     6601 atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc cacaggcgag
     6661 gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc ccggtttcag
     6721 aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc cctgaagggc
     6781 cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa cggcatctcc
     6841 aatcaggact ggctggccta catccaggag ctgcgcaacg gatccggcgc aacaaacttc
     6901 tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatggc caagcctttg
     6961 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     7021 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     7081 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     7141 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     7201 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     7261 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     7321 tctggttatg tgtgggaggg ctaagaattc gatatcaagc ttatcggtaa tcaacctctg
     7381 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7441 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7501 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7561 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7621 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7681 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7741 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7801 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7861 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7921 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     7981 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     8041 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     8101 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8161 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     8221 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     8281 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     8341 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     8401 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     8461 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8521 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8581 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8641 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     8701 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     8761 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     8821 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     8881 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     8941 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9001 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9061 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9121 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9181 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9241 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9301 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9361 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     9421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9481 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9541 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9601 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9661 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9721 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     9781 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     9841 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     9901 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     9961 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
    10021 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
    10081 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    10141 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    10201 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    10261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    10321 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    10381 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10441 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    10501 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10561 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10621 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10681 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    11041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    11101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    11161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    11221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    11281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11641 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11701 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11761 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11821 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11881 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11941 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    12001 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    12061 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    12121 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    12181 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    12241 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    12301 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    12361 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12421 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12481 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12541 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12601 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12661 gaaaagtgcc acctgac
//
