LOCUS Exported 10145 bp ds-DNA circular SYN 13-JUN-2023 DEFINITION Lentiviral expression of the cell cycle marker PIP-FUCCI. ACCESSION . VERSION . KEYWORDS pLenti-PGK-Neo-PIP-FUCCI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10145) AUTHORS Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE TITLE Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI. JOURNAL Cell Cycle. 2018;17(21-22):2496-2516. doi: 10.1080/15384101.2018.1547001. PUBMED 30421640 REFERENCE 2 (bases 1 to 10145) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10145 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 98..115 /label=L4440 /note="L4440 vector, forward primer" protein_bind 232..253 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 268..298 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 306..322 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 311..333 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 330..346 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 330..346 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 365..385 /label=T3 /note="T3 promoter, forward primer" promoter 367..385 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 413..639 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 640..820 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 867..992 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1485..1718 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1903..1947 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2214..2331 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2377..2887 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 2806..2824 /label=hPGK-F /note="Human PGK promoter, forward primer" protein_bind 2942..2966 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3038..3058 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3068..3094 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3098..3124 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3131..3844 /codon_start=1 /product="yellow fluorescent protein (YFP) with fast and efficient maturation (Nagai et al., 2002)" /label=Venus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELY" primer_bind complement(3176..3197) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3437..3456) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3784..3805 /label=EGFP-C /note="EGFP, forward primer" CDS 3860..3916 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 3923..4630 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(4068..4086) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 4336..4355 /label=mCherry-F /note="mCherry, forward primer" CDS 4637..4966 /codon_start=1 /product="degron consisting of residues 1-110 of human Geminin (Zielke and Edgar, 2015)" /label=Gem1 (1-110) /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE KRRKAL" protein_bind complement(4983..5007) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 5038..5626 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5091..5111) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5509..5520) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 5669..6168 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(5704..5727) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6101..6120 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6182..6985 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6245..6264) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6855..6874 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 7076..7309 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7381..7502 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7418..7437) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7472..7491 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7542..7677 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7604..7623 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7697..7716) /label=T7 /note="T7 promoter, forward primer" promoter complement(7698..7716) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7726..7743) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7726..7742) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7735..7757) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7884..8339 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7971..7990) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8181..8202 /label=F1ori-F /note="F1 origin, forward primer" promoter 8365..8469 /gene="bla" /label=AmpR promoter CDS 8470..9330 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8688..8707) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9501..10089 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9990..10009 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct 421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa 481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta 541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc 601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag 661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga 1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag 1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt 1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag 1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag 1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc 1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga 1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa 1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc 1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta 1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg 1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct 2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac 2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga 2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa 2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca 2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac 2341 gagactagcc tcgagaagct tgatatcgaa ttccacgggg ttggggttgc gccttttcca 2401 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag 2461 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt 2521 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg 2581 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac 2641 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc 2701 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga 2761 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg 2821 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc 2881 tccccagggg gatccaccgg ttcgtcgact agtccagtgt ggtggaattc tgcagatatc 2941 aacaagtttg tacaaaaaag caggcttcga attcatggag cagcgccgcg tcaccgactt 3001 cttcgcgcgc cgccgccccg ggccccggac acgtggccca aaaaagaaga gaaaggtagg 3061 agctcaatac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta 3121 tgcaactagt atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 3181 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 3241 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc 3301 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga 3361 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 3421 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 3481 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 3541 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa 3601 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt 3661 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 3721 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga 3781 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 3841 gtacctcgag ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga 3901 ggagaaccct ggacctcaat tgatggtgag caagggcgag gaggataaca tggccatcat 3961 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 4021 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 4081 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 4141 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 4201 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 4261 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 4321 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 4381 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 4441 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 4501 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 4561 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 4621 gctgtacaag gtcgagatga atcccagtat gaagcagaaa caagaagaaa tcaaagagaa 4681 tataaagaat agttctgtcc caagaagaac tctgaagatg attcagcctt ctgcatctgg 4741 atctcttgtt ggaagagaaa atgagctgtc cgcaggcttg tccaaaagga aacatcggaa 4801 tgaccactta acatctacaa cttccagccc tggggttatt gtcccagaat ctagtgaaaa 4861 taaaaatctt ggaggagtca cccaggagtc atttgatctt atgattaaag aaaatccatc 4921 ctctcagtat tggaaggaag tggcagaaaa acggagaaag gcgctgtaac aattgggatc 4981 ctacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgacaat 5041 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5101 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5161 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5221 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 5281 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 5341 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 5401 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 5461 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 5521 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 5581 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctgcag 5641 atatccggac tagtgatcta attctaccgg gtaggggagg cgcttttccc aaggcagtct 5701 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct 5761 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc 5821 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg 5881 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc 5941 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc 6001 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc 6061 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag 6121 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa 6181 tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 6241 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 6301 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 6361 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 6421 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 6481 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 6541 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 6601 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 6661 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 6721 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 6781 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 6841 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 6901 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 6961 tcgccttctt gacgagttct tctgagggga tccgtcgagg gcccggtacc tttaagacca 7021 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 7081 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 7141 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 7201 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 7261 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 7321 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 7381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 7441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 7501 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 7561 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 7621 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 7681 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 7741 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 7801 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 7861 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 7921 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 7981 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 8041 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 8101 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 8161 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 8221 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 8281 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 8341 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 8401 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 8461 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 8521 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 8581 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 8641 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 8701 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 8761 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 8821 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 8881 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 8941 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 9001 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 9061 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 9121 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 9181 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 9241 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 9301 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 9361 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 9421 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 9481 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 9541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 9601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 9661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 9721 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 9781 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 9841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 9901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 9961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 10021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 10081 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 10141 gctca //