LOCUS Exported 9018 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses proteins encoding Man operon in E. faecalis. ACCESSION . VERSION . KEYWORDS pMan operon SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9018) TITLE Bacterial expression plasmids for production of E. faecalis Man operon JOURNAL Unpublished REFERENCE 2 (bases 1 to 9018) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9018 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 5593..6181 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7685..8689) /codon_start=1 /gene="repB" /product="RepB replication protein" /label=repB /note="from Enterococcus faecalis plasmid pAM-alpha-1" /db_xref="GI:22652809" /protein_id="AAN03827.1" /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE" ORIGIN 1 tgtgctgttt tatcctttac cttgtctaca aaccccttaa aaacgttttt aaaggctttt 61 aagccgtctg tacgttcctt aaggaattcg agctcggtac ccggggatcc tctgctctta 121 atatggatat tccagcagaa gaagtttatt ttatcgcaca gttagtcaaa aatgcccagt 181 gtatcgaaga taaaatcacc gaaattgata cactagatta atacagaaaa atcgatacac 241 tatttattag tgtatcgatt ttttaatttt cttaaaaaaa cctataattt tctaggtttc 301 aaaccgatac actaaagttg gcacgaaact tgcttataat aaggtgtccc tttttaccta 361 aaatttttaa gtaaaaaaac aaaggtccta acctttaaaa atgaatttaa aggagcattc 421 ccgatggtaa tggatattcg tttagctcga attgatgatc gattaattca tggccaagta 481 gcaactgttt gggcaaaatt gacgaacatt aatcgcattt tagtggttag tgattccgtt 541 gctcatgaca atttgagaaa agccttgtta gttcaagctg ctccacctgg tgtcaaagta 601 aatgtcatca ctgttcaaaa aatgattgat gtttaccctg atcctcgttt tgattcgttt 661 cgagccatgt tgttattcac taatcctttg gacgtgaaac gagtggtcga aggcggtgta 721 caactaactt ctgtgaatat tggcggaatg agtttcagca caggcaagcg aatgattacg 781 aatgcagtcg cagtggatgc caacgattta aaagcatttc tgtttttaaa tgaacaaggt 841 attgaattgg aaattcgaaa agtcgcagca gacagccaag tgaatttgat ggatttactg 901 aaaaaagagc gtagcaaaga aaataacgcc agctgacgtt aatttttaca gacgaaagac 961 attgaaagga tgtggtggcc taggaccaac atcattgctt catgaactgt attgataaag 1021 gcaagtgctg atgttgaaaa aaatcaagaa ctattgaaat tttttcattt atttttgtca 1081 gaatctgatt tatctgattt ttttcaaaaa gtgaaagcgt ttagcagtac aaccatgaaa 1141 aaaaacatca attatacagg aggtataaaa atggtaggaa ttatcctagc aagtcacggt 1201 gaatttgctg aagggatctt gcaatctggt gcaatgatct ttggcgaaca agaaaatgta 1261 aaagcagtta cgttaatgcc tagcgaaggt cccgatgatg taaaagcaaa aatgcaagaa 1321 gccattgcat cgtttgacaa tcaagacgaa gtactatttt tagttgacct atggggagga 1381 acaccattta accaagcgaa ctcattgtta gaagatcata aagacaaatg ggcaatcgtt 1441 gcaggtatga acttaccaat ggttattgaa gcatatgctt ctcgtttctc tatggaatca 1501 gcacaagaaa ttgcgacaca cattcttgaa acagcaaaag atggtgtcaa agttaaacca 1561 gaagagttac aaccagcaga agcaccaaaa gctgctgcga cagaagatgc acagccaaaa 1621 ggttcgttac caccaggtac agttgttggc gatggcaaaa ttaaatttgt gctagcacgt 1681 attgattcac gtttattaca tggtcaagta gcaacagctt ggacaaaagc aacacaacca 1741 aacagaatta ttgttgtttc tgatgccgtt gcaaaagatg acttacgtaa gaaattaatc 1801 gaacaagcag ctcctccagg agtaaaagcc aatgttattc caatcagtaa aatgattgaa 1861 gtagcgaaag atccacgttt tggcaataca aaagcattat tattatttga aaatcctgaa 1921 gacgtcttaa aagtggttga aggcggcgta gaaatcccag aagtaaacgt tggttcaatg 1981 gctcactctg ttggaaaagt cgttgtaagc aaagtattat caatgggtca agaagacgtt 2041 gatacgtttg atgaattaaa agctaaaggc atcaaatttg atgtgcgtaa agtgccaaat 2101 gattcaaaag cgaatatgga cgaaattttg aaaaaagcaa aaaatgagtt agcaaacgcg 2161 taactcgcca aatacaaatc aaaataggag gcttatcatg tctgttatat caattatttt 2221 agttttactc gttgcctttc tagctggtat ggaagggatt ttggatgagt tccaattcca 2281 ccaaccgtta gtagcatgta cattaatcgg attagttact ggtaatttag aagccggaat 2341 tgttttaggt ggtactttac aaatgatcgc gttaggttgg gcgaacatcg gggctgccgt 2401 tgcaccggat gccgctttag catctgttgc ctcagcaatt attttagttt taggtggcca 2461 aggtgttaaa ggtgttcctt cagcgattgc catcgcagtt cctttagcgg ttgcaggttt 2521 gttcttaaca atgatcgttc ggacagttgc ggttccaatc gttcacatga tggatgcggc 2581 tgctgaaaaa ggcaacatta aacaagttga aatgttacac attttcgctg tatgtttaca 2641 aggtattcgt attgcgattc cagcaggtgc tttactattc attcctgctg acactgttcg 2701 taatttctta gaatcaatgc cagcttggtt aactgatggt atggctatcg gtggtggtat 2761 ggtcgttgcc gtaggttatg cattagtaat caacatgatg gcaacaaaag aagtatggcc 2821 attcttcatt atcggtttcg tagtcgcagc gatttctcaa ttaacattaa tcgcattagg 2881 tgctttaggt gtcgctttag ctcttattta cttgaactta tctaaaatgg gtggttcttc 2941 taactcaaat ggcggaggca actctggcga cccattaggc gatattttaa acgactacta 3001 attctgaagg aggagcaaag aaaatggcag aaaaaattca attaactaaa aaagatcgtt 3061 tagctgtagc ttggcgttca acatttatcc aaggttcttg gaactatgaa cgtatgcaaa 3121 acggcggctg ggcattctca atgattccag caatcaaaaa attatataaa acgaaagaag 3181 aacgttcagc ggcgttaaaa cgtcacttag aattctttaa tacacaccca tacattgcct 3241 caccaatcct tggtgtaaca ttagctttgg aagaagaacg tgccaatggc gcaccagttg 3301 atgacgtagc gattcaaggg gttaaagttg ggatgatggg tcctttagcc ggtgtcggtg 3361 acccagtttt ctggtttact gttcgtccaa tgttaggcgc tttaggtgct tccttagcaa 3421 tgggtggtaa catcttaggc ccaatcttat tcttcgttgc ttggaactta attcgttggt 3481 cattcatgtg gtatacacaa gagtttggtt acaaagcagg ttctaaaatt actgatgacc 3541 tttcaggtgg tttactacaa gatattacaa aaggtgcttc tattttaggg atgttcgtac 3601 tggccgcatt ggttcaacgt tgggtatcga ttaagttctt accaattgtt tcacaagtta 3661 agttagacaa aggtgcttat atcgagtggg ataaacttcc tgctggcggt gaaggaatgc 3721 ataaagcctt tgaacaagtg aaccaaggct tggcgctttc accaacgaaa gttactactt 3781 tacaagataa cctagatcaa ttgattccag gtctagcacc attactactt acattcttat 3841 gtatgtggtt attgaagaaa aaagttagcc caatcgttat cattcttggt ttattcgtag 3901 tcggcgtagt cggccactta atcggcttat tataagaatg atgaggtaag tttattactt 3961 ctaatcaaaa aaagcggaag agaagtcgag aaacttttct tccgcttttt taaacaaaga 4021 tgtaattttg tttgtttcga tcaggaaaga aacgtataat aaagaggaat tagtcgaatg 4081 aaatggagca gataacatgg tacaatcatt aaacacaaaa gttgatttag ttatagatgc 4141 gacggcattt acaggattaa cagattatgg acaaattatg ataggtgacc gcgggtttga 4201 attttaccat gccaaagatc cacgtaagtt tatccaaatt ccgtgggaag aagtcgatta 4261 cgtgttggct tcagtaatgt tcaaaggaaa atggattcct cgttatgcga ttcaaaccaa 4321 gaaaaatggg acgtatacgt ttgcttcaaa agagccgaag cgagtgttac gagctattcg 4381 tgaatatgtt gacccagatc atatggtcca atctttgagt ttctttgatg tcataaaaag 4441 agggatcctc tcgattggga aaaagaaaaa ataatttata actgttactt ttttataaac 4501 tagatgaaaa agtagcggtt tttttgtggg aaaacgaggg acttatttat acaaaaaagg 4561 cacaattttg caccaaaaaa gagacaatca agtgatacgt tgtttttaat ggagttatca 4621 ttgaaatggt tttcagaaag cacgatgaaa atatttgaca ccttgtaaaa tagtagtaac 4681 ataataagtg taataaatta ttttatagag gagtgagtgt catggaacaa cgacaacatc 4741 atggaatgga ctggggttca ttagtattag gaattctatt tgttctgact gcattaattt 4801 ctttccaaaa tcctgccggt aatttaattg ccattgtgat ggtttttgcg atttttgcta 4861 ttattaaagg gattttcgaa atttttgtac gtaatcgtat gaaagaattg ctaggctaca 4921 aagcttatgc accaatcatc ttagggatta ttgatatcct aattggtgtt tacttattgt 4981 ttaacttaaa tattggtgtc gctgtgttac catttgtttt tgcaatttgg tttttattcg 5041 actcaatttt cggcttattt actttagatt ttgctaaacg agtaagcaca ggttacttct 5101 ggttcacatt aattgttgac gttttaggga ttatcttagg tgtaatgtta ttatttaatc 5161 cattgtcttc agcattaact ctaagcttct tagttggctt ctactttatg atgtttggta 5221 ttagcaatat tgtatatgct tttagataaa agaagtaaag gaaaacctgt tttttttaat 5281 aacgacggcg gcagttgcca ttatttcccg aaattgttcg aataagccac acgataatcg 5341 ctacgggaac taaaatttta gagtcgacct gcaggcatgc aagcttggca ctggcggtgc 5401 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 5461 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5521 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 5581 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 5641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 5701 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 5761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 5821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 5881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 5941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6061 gcgcacgagg gagcttccag ggggaaacgc ctggtatcgt tatagtcctg tcgggtttcg 6121 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6181 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaggc 6241 aaccacttcc aagtaaagta taacacacta tactttatat tcataaagtg tgtgctctgc 6301 gaggctgtcg gcagtgccga ccaaaaccat aaaaccttta agacctttct tttttttacg 6361 agaaaaaaga aacaaaaaaa cctgccctct gccacctcag caaagggggg ttttgctctc 6421 gtgctcgttt aaaaatcagc aagggacagg tagtattttt tgagaagatc actcaaaaaa 6481 tctccacctt taaacccttg ccaattttta ttttgtccgt tttgtctagc ttaccgaaag 6541 ccagactcag caagaataaa atttttattg tctttcggtt ttctagtgta acggacaaaa 6601 ccactcaaaa taaaaaagat acaagagagg tctctcgtat cttttattca gcaatcggat 6661 ctggagctgt aatataaaaa ccttcttcaa ctaacggggc aggttagtga cattagaaaa 6721 ccgactgtaa aaagtacagt cggcattatc tcatattata aaagccagtc attaggccta 6781 tctgacaatt cctgaataga gttcataaac aatcctgcat gataaccatc acaaacagaa 6841 tgatgtacct gtaaagatag cggtaaatat attgaattac ctttattaat gaattttcct 6901 gctgtaataa tgggtagaag gtaattacta ttattattga tatttaagtt aaacccagta 6961 aatgaagtcc atggaataat agaaagagaa aaagcatttt caggtatagg tgttttggga 7021 aacaatttcc ccgaaccatt atatttctct acatcagaaa ggtataaatc ataaaactct 7081 ttgaagtcat tctttacagg agtccaaata ccagagaatg ttttagatac accatcaaaa 7141 attgtataaa gtggctctaa cttatcccaa taacctaact ctccgtcgct attgtaacca 7201 gttctaaaag ctgtatttga gtttatcacc cttgtcacta agaaaataaa tgcagggtaa 7261 aatttatatc cttcttgttt tatgtttcgg tataaaacac taatatcaat ttctgtggtt 7321 atactaaaag tcgtttgttg gttcaaataa tgattaaata tctcttttct cttccaattg 7381 tctaaatcaa ttttattaaa gttcatttga tatgcctcct aaatttttat ctaaagtgaa 7441 tttaggaggc ttacttgtct gctttcttca ttagaatcaa tcctttttta aaagtcaatg 7501 ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc 7561 tgtacagtca aggatggtag aaatgttgtc ggtcctatta ataactcgtc ttcctaagca 7621 tccttcaatc cttttaataa caattatagc atctaatctt caacaaactg gcccgtttgt 7681 tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca ataatagaaa 7741 atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt 7801 catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag attaaccttt 7861 tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct tcatcatcgg 7921 tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc gattgtatat 7981 ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga tcatagtcta 8041 atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag ttttctgtat 8101 tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta taagaattat 8161 ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt ttattaattt 8221 ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc ttatttaatt 8281 cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga actgttggct 8341 tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc attgctctcc 8401 tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac aactttcttt 8461 cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt actctttcag 8521 cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg attttctttt 8581 ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt gatttttcat 8641 ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc tatttagtta 8701 tttgtttagt cacttataac tttaacagat ggggtttttc tgtgcaacca attttaaggg 8761 ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca gcaactaaaa 8821 taaaaatgac gttatttcta tatgtatcaa gataagaaag aacaagttca aaaccatcaa 8881 aaaaagacac cttttcaggt gcttttttta ttttataaac tcattccctg atctcgactt 8941 cgttcttttt ttacctctcg gttatgagtt agttcaaatt cgttcttttt aggttctaaa 9001 tcgtgttttt cttggaat //