LOCUS Exported 4086 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS myrAkt delta4-129 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4086) AUTHORS Kohn AD, Takeuchi F, Roth RA TITLE Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. JOURNAL J Biol Chem. 1996 Sep 6. 271(36):21920-6. PUBMED 8702995 REFERENCE 2 (bases 1 to 4086) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4086 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 115..249 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(152..171) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 206..225 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(308..327) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(592..609) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(763..1351) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(843..862) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1522..2382) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2145..2164 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2383..2487) /gene="bla" /label=AmpR promoter primer_bind complement(2542..2562) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" primer_bind 2781..2800 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 2888..2897 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2894..2935 /codon_start=1 /product="N-myristoylation signal from Src kinase (Pellman et al., 1985; Kaplan et al., 1988)" /label=myr /translation="MGSSKSKPKDPSQR" CDS 3998..4024 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac 61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 241 gtctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt 301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc 361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc 421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta 481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgatatgc ggtgtgaaat 541 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 1021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 1081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 1141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 1201 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 1261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 1381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 1441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 1501 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 1561 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 1621 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 1681 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 1741 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 1801 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 1861 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 1921 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 1981 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 2041 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 2101 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 2161 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 2221 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 2281 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 2341 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 2401 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 2461 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaccttct 2521 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 2581 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg aaagtcccca 2641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 2701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 2761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 2821 ttccagaagt agtgaagagg cttttttgga ggagatctaa gcttgtcgac ggtaccccgg 2881 ggaattcgcc gccatgggga gtagcaagag caagcctaag gaccccagcc agcgcagcga 2941 ggctgaagag atggaggtgt ccctggccaa gcccaagcac cgcgtgacca tgaacgagtt 3001 tgagtacctg aagctgctgg gcaagggcac tttcggcaag gtgatcctgg tgaaggagaa 3061 ggccacaggc cgctactacg ccatgaagat cctcaagaag gaagtcatcg tggccaagga 3121 cgaggtggcc cacacactca ccgagaaccg cgtcctgcag aactccaggc accccttcct 3181 cacagccctg aagtactctt tccagaccca cgaccgcctc tgctttgtca tggagtacgc 3241 caacgggggc gagctgttct tccacctgtc ccgggaacgt gtgttctccg aggaccgggc 3301 ccgcttctat ggcgctgaga ttgtgtcagc cctggactac ctgcactcgg agaagaacgt 3361 ggtgtaccgg gacctcaagc tggagaacct catgctggac aaggacgggc acattaagat 3421 cacagacttc gggctgtgca aggaggggat caaggacggt gccaccatga agaccttttg 3481 cggcacacct gagtacctgg cccccgaggt gctggaggac aatgactacg gccgtgcagt 3541 ggactggtgg gggctgggcg tggtcatgta cgagatgatg tgcggtcgcc tgcccttcta 3601 caaccaggac catgagaagc tttttgagct catcctcatg gaggagatcc gcttcccgcg 3661 cacgcttggt cccgaggcca agtccttgct ttcagggctg ctcaagaagg accccaagca 3721 gaggcttggc gggggctccg aggacgccaa ggagatcatg cagcatcgct tctttgccgg 3781 tatcgtgtgg cagcacgtgt acgagaagaa gctcagccca cccttcaagc cccaggtcac 3841 gtcggagact gacaccaggt attttgatga ggagttcacg gcccagatga tcaccatcac 3901 accacctgac caagatgaca gcatggagtg tgtggacagc gagcgcaggc cccacttccc 3961 ccagttctcc tactcggcca gcagcacggc catggcttat ccttacgacg tccccgacta 4021 cgccagtctg ggacctggac tgtgaaacgg ttctcaccgc ggccgccacc gcggtggagc 4081 tctaga //