LOCUS       Exported                7736 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses human androgen receptor (R405S) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-AR-R405S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Lagarde WH, Blackwelder AJ, Minges JT, Hnat AT, French FS, Wilson EM
  TITLE     Androgen receptor exon 1 mutation causes androgen insensitivity by 
            creating phosphorylation site and inhibiting melanoma antigen-A11 
            activation of NH2- and carboxyl-terminal interaction-dependent 
            transactivation.
  JOURNAL   J Biol Chem. 2012 Mar 30;287(14):10905-15. doi: 
            10.1074/jbc.M111.336081. Epub 2012 Feb 13.
  PUBMED    22334658
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3760..4382
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3959..3978)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4406..4426)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4411..4740
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4591..4726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4653..4672
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4778..4797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4779..4797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4811..4827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4811..4827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4824..4846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4835..4851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4859..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4904..4925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5042..5059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5213..5801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5293..5312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5972..6832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6595..6614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6833..6937)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6964..7419
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7051..7070)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7261..7282
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7545..7567
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7559..7576
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7560..7576
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctgtccacc gtgtgtcttc ttctgcacga gactttgagg ctgtcagagc
      661 gctttttgcg tggttgctcc cgcaagtttc cttctctgga gcttcccgca ggtgggcagc
      721 tagctgcagc gactaccgca tcatcacagc ctgttgaact cttctgagca agagaagggg
      781 aggcggggta agggaagtag gtggaagatt cagccaagct caaggatgga agtgcagtta
      841 gggctgggaa gggtctaccc tcggccgccg tccaagacct accgaggagc tttccagaat
      901 ctgttccaga gcgtgcgcga agtgatccag aacccgggcc ccaggcaccc agaggccgcg
      961 agcgcagcac ctcccggcgc cagtttgctg ctgctgcagc agcagcagca gcagcagcag
     1021 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca agagactagc
     1081 cccaggcagc agcagcagca gcagggtgag gatggttctc cccaagccca tcgtagaggc
     1141 cccacaggct acctggtcct ggatgaggaa cagcaacctt cacagccgca gtcggccctg
     1201 gagtgccacc ccgagagagg ttgcgtccca gagcctggag ccgccgtggc cgccagcaag
     1261 gggctgccgc agcagctgcc agcacctccg gacgaggatg actcagctgc cccatccacg
     1321 ttgtccctgc tgggccccac tttccccggc ttaagcagct gctccgctga ccttaaagac
     1381 atcctgagcg aggccagcac catgcaactc cttcagcaac agcagcagga agcagtatcc
     1441 gaaggcagca gcagcgggag agcgagggag gcctcggggg ctcccacttc ctccaaggac
     1501 aattacttag ggggcacttc gaccatttct gacaacgcca aggagttgtg taaggcagtg
     1561 tcggtgtcca tgggcctggg tgtggaggcg ttggagcatc tgagtccagg ggaacagctt
     1621 cggggggatt gcatgtacgc cccacttttg ggagttccac ccgctgtgcg tcccactcct
     1681 tgtgccccat tggccgaatg caaaggttct ctgctagacg acagcgcagg caagagcact
     1741 gaagatactg ctgagtattc ccctttcaag ggaggttaca ccaaagggct agaaggcgag
     1801 agcctaggct gctctggcag cgctgcagca gggagctccg ggacacttga actgccgtct
     1861 accctgtctc tctacaagtc cggagcactg gacgaggcag ctgcgtacca gagtcgcgac
     1921 tactacaact ttccactggc tctggccgga ccgccgcccc ctccgccgcc tccccatccc
     1981 cacgctcgca tcaagctgga gaacccgctg gactacggca gcgcctgggc ggctgcggcg
     2041 gcgcagtgct cctatgggga cctggcgagc ctgcatggcg cgggtgcagc gggacccggt
     2101 tctgggtcac cctcagccgc cgcttcctca tcctggcaca ctctcttcac agccgaagaa
     2161 ggccagttgt atggaccgtg tggtggtggt gggggtggtg gcggcggcgg cggcggcggc
     2221 ggcggcggcg gcggcggcgg cggcggcggc gaggcgggag ctgtagcccc ctacggctac
     2281 actcggcccc ctcaggggct ggcgggccag gaaagcgact tcaccgcacc tgatgtgtgg
     2341 taccctggcg gcatggtgag cagagtgccc tatcccagtc ccacttgtgt caaaagcgaa
     2401 atgggcccct ggatggatag ctactccgga ccttacgggg acatgcgttt ggagactgcc
     2461 agggaccatg ttttgcccat tgactattac tttccacccc agaagacctg cctgatctgt
     2521 ggagatgaag cttctgggtg tcactatgga gctctcacat gtggaagctg caaggtcttc
     2581 ttcaaaagag ccgctgaagg gaaacagaag tacctgtgcg ccagcagaaa tgattgcact
     2641 attgataaat tccgaaggaa aaattgtcca tcttgtcgtc ttcggaaatg ttatgaagca
     2701 gggatgactc tgggagcccg gaagctgaag aaacttggta atctgaaact acaggaggaa
     2761 ggagaggctt ccagcaccac cagccccact gaggagacaa cccagaagct gacagtgtca
     2821 cacattgaag gctatgaatg tcagcccatc tttctgaatg tcctggaagc cattgagcca
     2881 ggtgtagtgt gtgctggaca cgacaacaac cagcccgact cctttgcagc cttgctctct
     2941 agcctcaatg aactgggaga gagacagctt gtacacgtgg tcaagtgggc caaggccttg
     3001 cctggcttcc gcaacttaca cgtggacgac cagatggctg tcattcagta ctcctggatg
     3061 gggctcatgg tgtttgccat gggctggcga tccttcacca atgtcaactc caggatgctc
     3121 tacttcgccc ctgatctggt tttcaatgag taccgcatgc acaagtcccg gatgtacagc
     3181 cagtgtgtcc gaatgaggca cctctctcaa gagtttggat ggctccaaat caccccccag
     3241 gaattcctgt gcatgaaagc actgctactc ttcagcatta ttccagtgga tgggctgaaa
     3301 aatcaaaaat tctttgatga acttcgaatg aactacatca aggaactcga tcgtatcatt
     3361 gcatgcaaaa gaaaaaatcc cacatcctgc tcaagacgct tctaccagct caccaagctc
     3421 ctggactccg tgcagcctat tgcgagagag ctgcatcagt tcacttttga cctgctaatc
     3481 aagtcacaca tggtgagcgt ggactttccg gaaatgatgg cagagatcat ctctgtgcaa
     3541 gtgcccaaga tcctttctgg gaaagtcaag cccatctatt tccacaccca gtgaagcatt
     3601 ggaaacccta tttccccacc ccagctcatg ccccctttca gatgtcttct gcctgttata
     3661 actctgcact actcctctgc agtgccttgg ggaatttcct ctattgatgt acagtctgtc
     3721 atgagcttgc atgcctgcag gtcgactcta gaggatcccg ggtggcatcc ctgtgacccc
     3781 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3841 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3901 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3961 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     4021 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4081 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4141 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4201 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     4261 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     4321 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     4381 ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4441 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4501 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4561 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4621 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4681 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4741 aagctcctcg aggaactgaa aaaccagaaa gttaattccc tatagtgagt cgtattaaat
     4801 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5401 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5641 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6961 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7021 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7081 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7141 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7201 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7261 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7321 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7381 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
     7441 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     7501 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     7561 taaaacgacg gccagtgcca agctgatcta tacattgaat caatattggc aattagccat
     7621 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     7681 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgtt
//
