LOCUS Exported 7715 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses human androgen receptor (R405A) in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-AR-R405A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7715) AUTHORS Lagarde WH, Blackwelder AJ, Minges JT, Hnat AT, French FS, Wilson EM TITLE Androgen receptor exon 1 mutation causes androgen insensitivity by creating phosphorylation site and inhibiting melanoma antigen-A11 activation of NH2- and carboxyl-terminal interaction-dependent transactivation. JOURNAL J Biol Chem. 2012 Mar 30;287(14):10905-15. doi: 10.1074/jbc.M111.336081. Epub 2012 Feb 13. PUBMED 22334658 REFERENCE 2 (bases 1 to 7715) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7715 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" polyA_signal 3739..4361 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3938..3957) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(4385..4405) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4390..4719 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4570..4705 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4632..4651 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4757..4776) /label=T7 /note="T7 promoter, forward primer" promoter complement(4758..4776) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4790..4806) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4790..4806) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4803..4825) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4814..4830 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4838..4868) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4883..4904 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5021..5038) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5192..5780) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5272..5291) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5951..6811) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6574..6593 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6812..6916) /gene="bla" /label=AmpR promoter rep_origin 6943..7398 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7030..7049) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7240..7261 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7524..7546 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7538..7555 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 7539..7555 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agaattcaga tctgtccacc gtgtgtcttc ttctgcacga gactttgagg ctgtcagagc 661 gctttttgcg tggttgctcc cgcaagtttc cttctctgga gcttcccgca ggtgggcagc 721 tagctgcagc gactaccgca tcatcacagc ctgttgaact cttctgagca agagaagggg 781 aggcggggta agggaagtag gtggaagatt cagccaagct caaggatgga agtgcagtta 841 gggctgggaa gggtctaccc tcggccgccg tccaagacct accgaggagc tttccagaat 901 ctgttccaga gcgtgcgcga agtgatccag aacccgggcc ccaggcaccc agaggccgcg 961 agcgcagcac ctcccggcgc cagtttgctg ctgctgcagc agcagcagca gcagcagcag 1021 cagcagcagc agcagcagca gcagcagcaa gagactagcc ccaggcagca gcagcagcag 1081 cagggtgagg atggttctcc ccaagcccat cgtagaggcc ccacaggcta cctggtcctg 1141 gatgaggaac agcaaccttc acagccgcag tcggccctgg agtgccaccc cgagagaggt 1201 tgcgtcccag agcctggagc cgccgtggcc gccagcaagg ggctgccgca gcagctgcca 1261 gcacctccgg acgaggatga ctcagctgcc ccatccacgt tgtccctgct gggccccact 1321 ttccccggct taagcagctg ctccgctgac cttaaagaca tcctgagcga ggccagcacc 1381 atgcaactcc ttcagcaaca gcagcaggaa gcagtatccg aaggcagcag cagcgggaga 1441 gcgagggagg cctcgggggc tcccacttcc tccaaggaca attacttagg gggcacttcg 1501 accatttctg acaacgccaa ggagttgtgt aaggcagtgt cggtgtccat gggcctgggt 1561 gtggaggcgt tggagcatct gagtccaggg gaacagcttc ggggggattg catgtacgcc 1621 ccacttttgg gagttccacc cgctgtgcgt cccactcctt gtgccccatt ggccgaatgc 1681 aaaggttctc tgctagacga cagcgcaggc aagagcactg aagatactgc tgagtattcc 1741 cctttcaagg gaggttacac caaagggcta gaaggcgaga gcctaggctg ctctggcagc 1801 gctgcagcag ggagctccgg gacacttgaa ctgccgtcta ccctgtctct ctacaagtcc 1861 ggagcactgg acgaggcagc tgcgtaccag agtcgcgact actacaactt tccactggct 1921 ctggccggac cgccgccccc tccgccgcct ccccatcccc acgctcgcat caagctggag 1981 aacccgctgg actacggcag cgcctgggcg gctgcggcgg cgcagtgcgc ctatggggac 2041 ctggcgagcc tgcatggcgc gggtgcagcg ggacccggtt ctgggtcacc ctcagccgcc 2101 gcttcctcat cctggcacac tctcttcaca gccgaagaag gccagttgta tggaccgtgt 2161 ggtggtggtg ggggtggtgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc 2221 ggcggcggcg aggcgggagc tgtagccccc tacggctaca ctcggccccc tcaggggctg 2281 gcgggccagg aaagcgactt caccgcacct gatgtgtggt accctggcgg catggtgagc 2341 agagtgccct atcccagtcc cacttgtgtc aaaagcgaaa tgggcccctg gatggatagc 2401 tactccggac cttacgggga catgcgtttg gagactgcca gggaccatgt tttgcccatt 2461 gactattact ttccacccca gaagacctgc ctgatctgtg gagatgaagc ttctgggtgt 2521 cactatggag ctctcacatg tggaagctgc aaggtcttct tcaaaagagc cgctgaaggg 2581 aaacagaagt acctgtgcgc cagcagaaat gattgcacta ttgataaatt ccgaaggaaa 2641 aattgtccat cttgtcgtct tcggaaatgt tatgaagcag ggatgactct gggagcccgg 2701 aagctgaaga aacttggtaa tctgaaacta caggaggaag gagaggcttc cagcaccacc 2761 agccccactg aggagacaac ccagaagctg acagtgtcac acattgaagg ctatgaatgt 2821 cagcccatct ttctgaatgt cctggaagcc attgagccag gtgtagtgtg tgctggacac 2881 gacaacaacc agcccgactc ctttgcagcc ttgctctcta gcctcaatga actgggagag 2941 agacagcttg tacacgtggt caagtgggcc aaggccttgc ctggcttccg caacttacac 3001 gtggacgacc agatggctgt cattcagtac tcctggatgg ggctcatggt gtttgccatg 3061 ggctggcgat ccttcaccaa tgtcaactcc aggatgctct acttcgcccc tgatctggtt 3121 ttcaatgagt accgcatgca caagtcccgg atgtacagcc agtgtgtccg aatgaggcac 3181 ctctctcaag agtttggatg gctccaaatc accccccagg aattcctgtg catgaaagca 3241 ctgctactct tcagcattat tccagtggat gggctgaaaa atcaaaaatt ctttgatgaa 3301 cttcgaatga actacatcaa ggaactcgat cgtatcattg catgcaaaag aaaaaatccc 3361 acatcctgct caagacgctt ctaccagctc accaagctcc tggactccgt gcagcctatt 3421 gcgagagagc tgcatcagtt cacttttgac ctgctaatca agtcacacat ggtgagcgtg 3481 gactttccgg aaatgatggc agagatcatc tctgtgcaag tgcccaagat cctttctggg 3541 aaagtcaagc ccatctattt ccacacccag tgaagcattg gaaaccctat ttccccaccc 3601 cagctcatgc cccctttcag atgtcttctg cctgttataa ctctgcacta ctcctctgca 3661 gtgccttggg gaatttcctc tattgatgta cagtctgtca tgagcttgca tgcctgcagg 3721 tcgactctag aggatcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc 3781 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt 3841 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag 3901 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt 3961 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg 4021 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg 4081 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct 4141 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct 4201 cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag 4261 cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc 4321 ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcttgg 4381 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 4441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 4561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4621 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 4681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga ggaactgaaa 4741 aaccagaaag ttaattccct atagtgagtc gtattaaatt cgtaatcatg tcatagctgt 4801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 4861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 4921 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 4981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 5101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 5161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 5281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 5401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 5461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 5641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 5761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 5821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 5881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 6541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 6901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg 6961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 7021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 7081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 7141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 7201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 7261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 7321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 7381 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc 7441 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 7501 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 7561 gctgatctat acattgaatc aatattggca attagccata ttagtcattg gttatatagc 7621 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat 7681 ttatattggc tcatgtccaa tatgaccgcc atgtt //