LOCUS Exported 13222 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells. ACCESSION . VERSION . KEYWORDS FLAG-FullLength-TET1-CXXC/LLAA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13222) AUTHORS Chandru A, Bate N, Vuister GW, Cowley SM TITLE Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain. JOURNAL Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w. PUBMED 30279502 REFERENCE 2 (bases 1 to 13222) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13222 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..63 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" promoter 516..727 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 740..1008 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 1071..1094 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" misc_feature 7404..7990 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7571..7588) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7798..7817 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8003..8797 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8057..8076) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8667..8686 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(8857..8877) /label=WPRE-R /note="WPRE, reverse primer" polyA_signal 8919..9040 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8956..8975) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9010..9029 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" repeat_region 9803..9837 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(10328..10344) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10328..10344) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10341..10363) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10352..10368 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10376..10406) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10421..10442 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10559..10576) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10730..11318) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10810..10829) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11489..12349) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12112..12131 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12350..12454) /gene="bla" /label=AmpR promoter rep_origin complement(12480..12935) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(12617..12638) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 12829..12848 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 13062..13084 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 13076..13093 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 13077..13093 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgtgta aaattgacgc 61 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt 121 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt 181 tatttattaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttatga 241 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc 301 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg 361 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg 421 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa ggcctccacg 481 gccaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 541 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 601 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 661 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 721 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 781 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 841 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 901 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 961 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 1021 ggcgcctact ctagagctag ccaaccatgg attacaagga tcatgacatt gactacaaag 1081 acgatgacga caagggcggt tccatgtctc gatcccgcca tgcaaggcct tccagattag 1141 tcaggaagga agatgtaaac aaaaaaaaga aaaacagcca actacgaaag acaaccaagg 1201 gagccaacaa aaatgtggca tcagtcaaga ctttaagccc tggaaaatta aagcaattaa 1261 ttcaagaaag agatgttaag aaaaaaacag aacctaaacc acccgtgcca gtcagaagcc 1321 ttctgacaag agctggagca gcacgcatga atttggatag gactgaggtt ctttttcaga 1381 acccagagtc cttaacctgc aatgggttta caatggcgct acgaagcacc tctcttagca 1441 ggcgactctc ccaaccccca ctggtcgtag ccaaatccaa aaaggttcca ctttctaagg 1501 gtttagaaaa gcaacatgat tgtgattata agatactccc tgctttggga gtaaagcact 1561 cagaaaatga ttcggttcca atgcaagaca cccaagtcct tcctgatata gagactctaa 1621 ttggtgtaca aaatccctct ttacttaaag gtaagagcca agagacaact cagttttgga 1681 gccaaagagt tgaggattcc aagatcaata tccctaccca cagtggccct gcagctgaga 1741 tccttcctgg gccactggaa gggacacgct gtggtgaagg actattctct gaagagacat 1801 tgaatgatac cagtggttcc ccaaaaatgt ttgctcagga cacagtgtgt gctccttttc 1861 cccaaagagc cacccccaaa gttacctctc aaggaaaccc cagcattcag ttagaagagt 1921 tgggttcacg agtagaatct cttaagttat ctgattctta cctggatccc attaaaagtg 1981 aacatgattg ctaccccacc tccagtctta ataaggttat acctgacttg aaccttagaa 2041 actgcttggc tcttggtggg tctacgtctc ctacctctgt aataaaattc ctcttggcag 2101 gctcaaaaca agcgaccctt ggtgctaaac cagatcatca agaggccttc gaagctactg 2161 caaatcaaca ggaagtttct gataccacct ctttcctagg acaggccttt ggtgctatcc 2221 cacatcaatg ggaacttcct ggtgctgacc cagttcatgg tgaggccctg ggtgagaccc 2281 cagatctacc agagattcct ggtgctattc cagtccaagg agaggtcttt ggtactattt 2341 tagaccaaca agaaactctt ggtatgagtg ggagtgttgt cccagacttg cctgtcttcc 2401 ttcctgttcc tccaaatcca attgctacct ttaatgctcc ttccaaatgg cctgagcccc 2461 aaagcactgt ctcatatgga cttgcagtcc agggtgctat acagattttg cctttgggct 2521 caggacacac tcctcaatca tcatcaaact cagagaaaaa ttcattacct ccagtaatgg 2581 ctataagcaa tgtagaaaat gagaagcagg ttcatataag cttcctgcca gctaacactc 2641 aggggttccc attagcccct gagagaggac tcttccatgc ttcactgggt atagcccaac 2701 tctctcaggc tggtcctagc aaatcagaca gagggagctc ccaggtcagt gtaaccagca 2761 cagttcatgt tgtcaacacc acagtggtga ctatgccagt gccaatggtc agtacctcct 2821 cttcttccta taccactttg ctaccgactt tggaagagga gctgaaaaag aaaccatctg 2881 ttgttgtgcc tctggaggtt ataaaggaaa acaagaggcc ccagagggaa aagaagccca 2941 aagttttaaa ggcagatttt gacaacaaac cagtaaatgg ccccaagtca gaatccatgg 3001 actacagtag atgtggtcat ggggaagaac aaaaattgga attgaaccca catactgttg 3061 aaaatgtaac taaaaatgaa gacagcatga caggcatcga ggtggagaag tggacacaaa 3121 acaagaaatc acagttaact gatcacgtga aaggagattt tagtgctaat gtcccagaag 3181 ctgaaaaatc gaaaaactct gaagttgaca agaaacgaac caaatctcca aaattgtttg 3241 tacaaaccgt aagaaatggc attaaacatg tacactgttt accagctgaa acaaatgttt 3301 catttaaaaa attcaatatt gaagaattcg gcaagacatt ggaaaacaat tcttataaat 3361 tcctaaaaga cactgcaaac cataaaaacg ctatgagctc tgttgctact gatatgagtt 3421 gtgatcatct caaggggaga agtaacgttt tagtattcca gcagcctggc tttaactgca 3481 gttccattcc acattcttca cactccatca taaatcatca tgctagtata cacaatgaag 3541 gtgatcaacc aaaaactcct gagaatatac caagtaaaga accaaaagat ggatctcccg 3601 ttcaaccaag tctcttatcg ttaatgaaag ataggagatt aacattggag caagtggtag 3661 ccatagaggc cgccactcaa gcctcagaag ccccatcaga gaattcctcc ccatcaaagt 3721 cagagaagga tgaggaatca gagcagagaa cagccagttt gcttaatagc tgcaaagcta 3781 tcctctacac tgtaagaaaa gacctccaag acccaaactt acagggagag ccaccaaaac 3841 ttaatcactg tccatctttg gaaaaacaaa gttcatgcaa cacggtggtt ttcaatgggc 3901 aaactactac cctttccaac tcacatatca actcagctac taaccaagca tccacaaagt 3961 cacatgaata ttcaaaagtc acaaattcat tatctctttt tataccaaaa tcaaattcat 4021 ccaagattga caccaataaa agtattgctc aagggataat tactcttgac aattgttcca 4081 atgatttgca tcagttgcca ccaagaaata atgaagtgga gtattgcaac cagttactgg 4141 acagcagcaa aaaattggac tcagatgatc tatcatgtca ggatgcaacc catacccaaa 4201 ttgaggaaga tgttgcaaca cagttgacac aacttgcttc gataattaag atcaattata 4261 taaaaccaga ggacaaaaaa gttgaaagta caccaacaag ccttgtcaca tgtaatgtac 4321 agcaaaaata caatcaggag aagggcacaa tccaacagaa accaccttca agtgtacaca 4381 ataatcatgg ttcatcatta acaaaacaaa agaacccaac ccagaaaaag acaaaatcca 4441 ccccatcaag agatcggcgg aaaaagaagc ccacagttgt aagttatcaa gaaaatgatc 4501 ggcagaagtg ggaaaagttg tcctatatgt atggcacaat atgcgacatt tggatagcat 4561 cgaaatttca aaattttggg caattttgtc cacatgattt tcctactgta tttgggaaaa 4621 tttcttcctc gaccaaaata tggaaaccac tggctcaaac gaggtccatt atgcaaccca 4681 aaacagtatt tccaccactc actcagataa aattacagag atatcctgaa tcagcagagg 4741 aaaaggtgaa ggttgaacca ttggattcac tcagcttatt tcatcttaaa acggaatcca 4801 acgggaaggc attcactgat aaagcttata attctcaggt acagttaacg gtgaatgcca 4861 atcagaaagc ccatcctttg acccagccct cctctccacc taaccagtgt gctaacgtga 4921 tggcaggcga tgaccaaata cggtttcagc aggttgttaa ggagcaactc atgcatcaga 4981 gactgccaac attgcctggt atctctcatg aaacaccctt accggagtca gcactaactc 5041 tcaggaatgt aaatgtagtg tgttcaggtg gaattacagt ggtttctacc aaaagtgaag 5101 aggaagtctg ttcatccagt tttggaacat cagaattttc cacagtggac agtgcacaga 5161 aaaattttaa tgattatgcc atgaacttct ttactaaccc tacaaaaaac ctagtgtcta 5221 taactaaaga ttctgaactg cccacctgca gctgtcttga tcgagttata caaaaagaca 5281 aaggcccata ttatacacac cttggggcag gaccaagtgt tgctgctgtc agggaaatca 5341 tggagaatag gtatggtcaa aaaggaaacg caataaggat agaaatagta gtgtacaccg 5401 gtaaagaagg gaaaagctct catgggtgtc caattgctaa gtgggtttta agaagaagca 5461 gtgatgaaga aaaagttctt tgtttggtcc ggcagcgtac aggccaccac tgtccaactg 5521 ctgtgatggt ggtgctcatc atggtgtggg atggcatccc tcttccaatg gccgaccggc 5581 tatacacaga gctcacagag aatctaaagt catacaatgg gcaccctacc gacagaagat 5641 gcaccctcaa tgaaaatcgt acctgtacat gtcaaggaat tgatccagag acttgtggag 5701 cttcattctc ttttggctgt tcatggagta tgtactttaa tggctgtaag tttggtagaa 5761 gcccaagccc cagaagattt agaattgatc caagctctcc cttacatgaa aaaaaccttg 5821 aagataactt acagagtttg gctacacgat tagctccaat ttataagcag tatgctccag 5881 tagcttacca aaatcaggtg gaatatgaaa atgttgcccg agaatgtcgg cttggcagca 5941 aggaaggtcg tcccttctct ggggtcactg cttgcctgga cttctgtgct catccccaca 6001 gggacattca caacatgaat aatggaagca ctgtggtttg taccttaact cgagaagata 6061 accgctcttt gggtgttatt cctcaagatg agcagctcca tgtgctacct ctttataagc 6121 tttcagacac agatgagttt ggctccaagg aaggaatgga agccaagatc aaatctgggg 6181 ccatcgaggt cctggcaccc cgccgcaaaa aaagaacgtg tttcactcag cctgttcccc 6241 gttctggaaa gaagagggct gcgatgatga cagaggttct tgcacataag ataagggcag 6301 tggaaaagaa acctattccc cgaatcaagc ggaagaataa ctcaacaaca acaaacaaca 6361 gtaagccttc gtcactgcca accttaggga gtaacactga gaccgtgcaa cctgaagtaa 6421 aaagtgaaac cgaaccccat tttatcttaa aaagttcaga caacactaaa acttattcgc 6481 tgatgccatc cgctcctcac ccagtgaaag aggcatctcc aggcttctcc tggtccccga 6541 agactgcttc agccacacca gctccactga agaatgacgc aacagcctca tgcgggtttt 6601 cagaaagaag cagcactccc cactgtacga tgccttcggg aagactcagt ggtgccaatg 6661 cagctgctgc tgatggccct ggcatttcac agcttggcga agtggctcct ctccccaccc 6721 tgtctgctcc tgtgatggag cccctcatta attctgagcc ttccactggt gtgactgagc 6781 cgctaacgcc tcatcagcca aaccaccagc cctccttcct cacctctcct caagaccttg 6841 cctcttctcc aatggaagaa gatgagcagc attctgaagc agatgagcct ccatcagacg 6901 aacccctatc tgatgacccc ctgtcacctg ctgaggagaa attgccccac attgatgagt 6961 attggtcaga cagtgagcac atctttttgg atgcaaatat tggtggggtg gccatcgcac 7021 ctgctcacgg ctcggttttg attgagtgtg cccggcgaga gctgcacgct accactcctg 7081 ttgagcaccc caaccgtaat catccaaccc gcctctccct tgtcttttac cagcacaaaa 7141 acctaaataa gccccaacat ggttttgaac taaacaagat taagtttgag gctaaagaag 7201 ctaagaataa gaaaatgaag gcctcagagc aaaaagacca ggcagctaat gaaggtccag 7261 aacagtcctc tgaagtaaat gaattgaacc aaattccttc tcataaagca ttaacattaa 7321 cccatgacaa tgttgtcacc gtgtcccctt atgctctcac acacgttgcg gggccctata 7381 accattgggt ctaagcggcc gcgcccctct ccctcccccc cccctaacgt tactggccga 7441 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 7501 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 7561 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 7621 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 7681 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 7741 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 7801 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 7861 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 7921 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat 7981 ggccacaacc atggcgtccg gaatgattga acaagatgga ttgcacgcag gttctccggc 8041 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 8101 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 8161 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccgcgac 8221 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 8281 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 8341 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 8401 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 8461 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 8521 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 8581 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 8641 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 8701 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 8761 catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac ctctggatta 8821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 8881 atacgctgct ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt gcagcttata 8941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 9001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg aattgactca 9061 aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg acaagacatc 9121 cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc ttgctctggt 9181 caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga aacagtggca 9241 gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg tatggctttt 9301 tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa 9361 gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat 9421 gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg 9481 ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga tatctataac 9541 aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta 9601 tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg 9661 cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag 9721 ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag 9781 agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta 9841 atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc 9901 tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg 9961 gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg 10021 tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt 10081 caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc 10141 ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg 10201 cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc 10261 tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg cgcttggcgt 10321 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 10381 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 10441 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 10501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 10561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 10621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 10681 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 10741 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 10801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 10861 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 10921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 10981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 11041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 11101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 11161 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 11221 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 11281 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 11341 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 11401 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 11461 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 11521 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 11581 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 11641 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 11701 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 11761 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 11821 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 11881 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 11941 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 12001 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 12061 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 12121 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 12181 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 12241 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 12301 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 12361 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 12421 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta 12481 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 12541 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 12601 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 12661 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 12721 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 12781 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 12841 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 12901 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa 12961 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 13021 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 13081 aacgacggcc agtgagcgcg cctcgttcat tcacgttttt gaacccgtgg aggacgggca 13141 gactcgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct 13201 gcagaacacg cagctagatt aa //