LOCUS Exported 11695 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of PIK3CA N1068Kfs*5. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CA-N1068Kfs*5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11695) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11695) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11695 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6655..6679) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6687..6705 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6730..6749) /label=T7 /note="T7 promoter, forward primer" promoter complement(6731..6749) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6755..6775) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6829..6870 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6891..7458 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6908..7460 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7052..7069) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7279..7298 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7472..8071 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7472..7491) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7968..7988 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8085..8673 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8138..8158) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8556..8567) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8748..8981 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9100..9118) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9186..9290 /gene="bla" /label=AmpR promoter CDS 9291..10151 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9509..9528) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10322..10910 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10811..10830 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11064..11081 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11173..11192 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11321..11342 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11357..11387 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11395..11411 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11400..11422 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11419..11435 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11419..11435 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11485..11504 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg 3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata 3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa 3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta 3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac 3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag 3781 atcctcaatc gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt tgatatggtt 3841 aaagatccag aagtacagga cttccgaaga aatattctga acgtttgtaa agaagctgtg 3901 gatcttaggg acctcaattc acctcatagt agagcaatgt atgtctatcc tccaaatgta 3961 gaatcttcac cagaattgcc aaagcacata tataataaat tagataaagg gcaaataata 4021 gtggtgatct gggtaatagt ttctccaaat aatgacaagc agaagtatac tctgaaaatc 4081 aaccatgact gtgtaccaga acaagtaatt gctgaagcaa tcaggaaaaa aactcgaagt 4141 atgttgctat cctctgaaca actaaaactc tgtgttttag aatatcaggg caagtatatt 4201 ttaaaagtgt gtggatgtga tgaatacttc ctagaaaaat atcctctgag tcagtataag 4261 tatataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa 4321 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt 4381 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat 4441 agtgcactca gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt 4501 gataagatct atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg 4561 aacactcaaa gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata 4621 tacattcctg atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc 4681 cgaaagggtg ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat 4741 tacacagaca ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctcatgga 4801 ttagaagatt tgctgaaccc tattggtgtt actggatcaa atccaaataa agaaactcca 4861 tgcttagagt tggagtttga ctggttcagc agtgtggtaa agttcccaga tatgtcagtg 4921 attgaagagc atgccaattg gtctgtatcc cgagaagcag gatttagcta ttcccacgca 4981 ggactgagta acagactagc tagagacaat gaattaaggg aaaatgacaa agaacagctc 5041 aaagcaattt ctacacgaga tcctctctct gaaatcactg agcaggagaa agattttcta 5101 tggagtcaca gacactattg tgtaactatc cccgaaattc tacccaaatt gcttctgtct 5161 gttaaatgga attctagaga tgaagtagcc cagatgtatt gcttggtaaa agattggcct 5221 ccaatcaaac ctgaacaggc tatggaactt ctggactgta attacccaga tcctatggtt 5281 cgaggttttg ctgttcggtg cttggaaaaa tatttaacag atgacaaact ttctcagtat 5341 ttaattcagc tagtacaggt cctaaaatat gaacaatatt tggataactt gcttgtgaga 5401 tttttactga agaaagcatt gactaatcaa aggattgggc actttttctt ttggcattta 5461 aaatctgaga tgcacaataa aacagttagc cagaggtttg gcctgctttt ggagtcctat 5521 tgtcgtgcat gtgggatgta tttgaagcac ctgaataggc aagtcgaggc aatggaaaag 5581 ctcattaact taactgacat tctcaaacag gagaagaagg atgaaacaca aaaggtacag 5641 atgaagtttt tagttgagca aatgaggcga ccagatttca tggatgctct acagggcttt 5701 ctgtctcctc taaaccctgc tcatcaacta ggaaacctca ggcttgaaga gtgtcgaatt 5761 atgtcctctg caaaaaggcc actgtggttg aattgggaga acccagacat catgtcagag 5821 ttactgtttc agaacaatga gatcatcttt aaaaatgggg atgatttacg gcaagatatg 5881 ctaacacttc aaattattcg tattatggaa aatatctggc aaaatcaagg tcttgatctt 5941 cgaatgttac cttatggttg tctgtcaatc ggtgactgtg tgggacttat tgaggtggtg 6001 cgaaattctc acactattat gcaaattcag tgcaaaggcg gcttgaaagg tgcactgcag 6061 ttcaacagcc acacactaca tcagtggctc aaagacaaga acaaaggaga aatatatgat 6121 gcagccattg acctgtttac acgttcatgt gctggatact gtgtagctac cttcattttg 6181 ggaattggag atcgtcacaa tagtaacatc atggtgaaag acgatggaca actgtttcat 6241 atagattttg gacacttttt ggatcacaag aagaaaaaat ttggttataa acgagaacgt 6301 gtgccatttg ttttgacaca ggatttctta atagtgatta gtaaaggagc ccaagaatgc 6361 acaaagacaa gagaatttga gaggtttcag gagatgtgtt acaaggctta tctagctatt 6421 cgacagcatg ccaatctctt cataaatctt ttctcaatga tgcttggctc tggaatgcca 6481 gaactacaat cttttgatga cattgcatac attcgaaaga ccctagcctt agataaaact 6541 gagcaagagg ctttggagta tttcatgaaa caaatgaatg atgcacatca tggtggctgg 6601 acaacaaaaa tggattggat cttccacaca attaaacagc atgcattgaa actgacccaa 6661 ctttcttgta caaagtggtg atatccaatt aaccctcact aaaggaaaga attagtatga 6721 tagatgagac cctatagtga gtcgtattac cacccaactt ttctatacaa agtggttgat 6781 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc 6841 cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga tccctccccc 6901 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg 6961 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc 7021 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg 7081 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg 7141 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 7201 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 7261 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc 7321 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt 7381 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt 7441 tgaaaaacac gatgataata tggccacaca tatgaccgag tacaagccca cggtgcgcct 7501 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 7561 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 7621 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 7681 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 7741 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 7801 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 7861 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 7921 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 7981 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 8041 ctggtgcatg acccgcaagc ccggtgccta aatcgataga tcctaatcaa cctctggatt 8101 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 8161 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 8221 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 8281 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 8341 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 8401 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 8461 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 8521 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 8581 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 8641 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta 8701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 8761 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 8821 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 8881 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8941 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 9001 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt 9061 aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt 9121 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 9181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 9241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 9301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 9361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 9421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 9481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg 9541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 9601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 9661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 9721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 9781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 9841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 9901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 9961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 10021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 10081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 10141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 10201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 10261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 10321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 10381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 10441 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 10501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 10561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 10621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 10681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 10741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 10801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 10861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 10921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 10981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 11041 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 11101 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg 11161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 11221 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca 11281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 11341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 11401 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag 11461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 11521 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc 11581 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat 11641 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //