LOCUS Exported 11196 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector with UBC promoter. ACCESSION . VERSION . KEYWORDS pLEX-MCS UBC promoter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11196) TITLE Emory Custom Cloning Core Plasmids - Oskar Laur REFERENCE 2 (bases 1 to 11196) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11196 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2063..2180 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(2245..2619) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" regulatory 2616..2625 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(2629..2647) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(2639..2686) /label=EM7 promoter /note="synthetic bacterial promoter " promoter 2718..3929 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3766..3785 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" misc_feature 3987..4559 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4154..4171) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4381..4400 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4562..5164 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 5061..5081 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5171..5759 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5224..5244) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5642..5653) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5966..6199 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6300..6317) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6306..6530 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6576..7004 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6663..6682) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6873..6894 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7013..7033) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7018..7347 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7198..7333 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7260..7279 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7396..8421 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" polyA_signal 8551..8672 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8721..8737) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8721..8737) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8734..8756) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8745..8761 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8769..8799) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8814..8835 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8952..8969) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9123..9711) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9203..9222) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9882..10742) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10505..10524 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10743..10847) /gene="bla" /label=AmpR promoter polyA_signal 10895..11029 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc 2701 cgaggttcaa gagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct 2761 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc 2821 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca 2881 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga 2941 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg 3001 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc 3061 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag 3121 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa 3181 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact 3241 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct 3301 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc 3361 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg 3421 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct 3481 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg 3541 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc 3601 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag 3661 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc 3721 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt 3781 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa 3841 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat 3901 tctggccgtt tttggctttt ttgttagacg gatccccata cgtagtgctc gagtctagag 3961 ggcccgacgc gtctggccac cggtcgcccc tctccctccc ccccccctaa cgttactggc 4021 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 4081 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 4141 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 4201 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 4261 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 4321 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 4381 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 4441 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 4501 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac 4561 catggccacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc 4621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc 4681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 4741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 4801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag 4861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 4921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 4981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt 5041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 5101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 5161 ctgagttaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 5221 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 5281 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 5341 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 5401 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 5461 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 5521 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 5581 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 5641 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 5701 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 5761 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca 5821 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt 5881 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5941 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat 6001 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6181 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6241 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc gctgatcagc 6301 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 6361 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 6421 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 6481 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 6541 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 6601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 6661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 6721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 6781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 6841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 6901 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 6961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 7021 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 7081 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 7141 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 7201 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7261 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 7321 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 7381 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg 7441 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt 7501 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat 7561 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg 7621 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca 7681 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc 7741 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca 7801 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct 7861 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg 7921 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg 7981 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt 8041 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg 8101 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag 8161 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat 8221 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca 8281 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc 8341 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact 8401 cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc cgccttctat 8461 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 8521 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 8581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 8641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 8701 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 8761 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 8821 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 8881 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 8941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 9001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 9061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 9121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 9181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 9241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 9301 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 9361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 9421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 9481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 9541 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 9601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 9661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 9721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 9781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 9841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 9901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 9961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 10021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 10081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 10141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 10201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 10261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 10321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 10381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 10441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 10501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 10561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 10621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 10681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 10741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 10801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 10861 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 10921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 10981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 11041 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 11101 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 11161 ttgtatttgt taaatatgta ctacaaactt agtagt //