LOCUS Exported 4421 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express GABAR-alpha-1 N terminal domain. ACCESSION . VERSION . KEYWORDS pCMV-GABAR-alpha-1N-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4421) AUTHORS Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, Sillitoe RV, Jiang X, Zoghbi HY TITLE Neurexophilin4 is a selectively expressed alpha-neurexin ligand that modulates specific cerebellar synapses and motor functions. JOURNAL Elife. 2019 Sep 16;8. pii: 46773. doi: 10.7554/eLife.46773. PUBMED 31524598 REFERENCE 2 (bases 1 to 4421) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4421 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 27..330 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 331..534 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 485..505 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 531..555 /label=LNCX /note="Human CMV promoter, forward primer" intron 672..768 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" CDS 1519..1545 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 1560..1694 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1584..1603) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1638..1657 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1805..1821) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1805..1821) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1818..1840) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1829..1845 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1853..1883) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1898..1919 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2036..2053) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2207..2795) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2287..2306) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2966..3826) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3589..3608 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3827..3931) /gene="bla" /label=AmpR promoter primer_bind 3999..4017 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4055..4077) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4177..4196 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4390..4412 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4404..4421 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4405..4421 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcatg 841 ctcgaggatg aacttaaaga caacaccact gtcttcacaa gaattttgga ccgactgctg 901 gacggttatg acaatcgtct gagaccaggt ttgggagagc gtgtaactga agtgaagacc 961 gacattttcg tcaccagttt cggaccagtt tcagaccacg atatggagta tacaatagat 1021 gtgtttttcc gtcaaagttg gaaggatgaa agattaaaat tcaaaggacc catgacagtg 1081 ctccggctaa acaaccttat ggccagtaaa atctggactc cagatacatt tttccacaat 1141 ggaaagaagt ctgtggccca caacatgacc atgcctaata agctcctgcg tatcacagag 1201 gatggcactc tgctgtacac catgaggttg accgtgagag ctgaatgccc aatgcacttg 1261 gaagacttcc ctatggatgc ccatgcctgc ccactaaaat tcggaagcta tgcttataca 1321 agagcagaag ttgtctatga gtggaccaga gagccagccc gttcagtggt tgtagcagaa 1381 gatgggtcac gtttaaacca gtatgacctt cttggacaaa cagttgactc tggaattgtt 1441 cagtccagta ctggagaata tgtggttatg acgactcact tccacttgaa gagaaaaatt 1501 ggctctcgag gtaccggcta cccatacgat gttccagatt acgcttaagc ggccgcgggg 1561 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 1621 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 1681 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg 1741 tgtgggaggt tttttcggat cctctagagt cgatctgcag gcatgctagc ttggcgtaat 1801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 1921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 1981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 2041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 2881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 3001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 3661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 3721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 3781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 3901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 3961 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 4021 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 4081 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 4141 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 4201 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 4261 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 4321 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 4381 cagggttttc ccagtcacga cgttgtaaaa cgacggccag t //